Use of microautoradiography and fluorescent in situ hybridization for characterization of microbial activity in activated sludge

Water Science and Technology

Volumn 39, Issue 1, 1999, Pages 1-9

  Nielsen, P H ^a   Andreasen, K ^b   Lee, N ^a,c   Wagner, M ^c  

^a AALBORG UNIVERSITY   (Denmark)

^b Kruger AS   (Denmark)

^c TECHNICAL UNIVERSITY OF MUNICH   (Germany)

Author keywords

16S rRNA probes; Enumeration; Fluorescent in situ hybridization; In situ physiology; Microautoradiography

Indexed keywords

ACTIVATED SLUDGE; MICROORGANISM;

ACTIVATED SLUDGE; AUTORADIOGRAPHY; CONFERENCE PAPER; FLUORESCENCE IN SITU HYBRIDIZATION; MATHEMATICAL MODEL; MICROBIAL GROWTH; MICROSCOPY;

EID: 0032917393     PISSN: 02731223     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0273-1223(98)00770-7     Document Type: Conference Paper

References (33)

1. Amann, R., Ludwig, W. and Schleifer, K.-H. (1995). Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol. Rev., 59, 143-169.
2. Andreasen, K, and Nielsen, P. H. (1997). Application of microautoradiography for the study of substrate uptake by filamentous microorganisms in activated sludge. Appl. Environ. Microbiol., 63, 3662-3668.
3. Andreasen, K. and Nielsen, P. H. (1998). In situ characterization of the substrate uptake by Microthrix parvicella by using microautoradiography. Wat. Sci. Tech., 37(4-5), 19-26.
4. Andreasen, K. and Nielsen, P. H. (submitted). Investigation of in situ phosphorus uptake by Microthrix parvicella in activated sludge.
5. Brock, T. D. and Brock, M. L. (1968). Autoradiography as a tool in microbial ecology. Nature, 209, 734-736.
6. Bourdier, G., Bohatier, M. and Feuillade, J. (1989). Amino acid incorporation by natural population of Oscillatoria rubescens. A microautoradiographic study. FEMS Microbiol. Ecol., 62, 185-190.
7. Carman, K. R. (1990). Radioactive labelling of a natural assemblage of marine sedimentary bacteria and microalgae for the trophic studies: An autoradiographic study. Microb. Ecol., 19, 279-290.
8. Hermansson, M. and Dahlbäck, B. (1983). Bacterial activity at the air/water interface. Microb. Ecol., 9, 317-328.
9. Hoppe, H.-G. (1976). Determination and properties of actively metabolizing heterotrophic bacteria in the sea, investigated by means of micro-autoradiography. Marine Biology, 36, 291-302
10. Lee, N., Andreasen, K., Wagner, M. and Nielsen, P. H. (in preparation). Combination of MAR and FISH - a new tool in microbial ecology.
11. Meyer-Reil, L.-A. (1978). Autoradiography and epifluorescence microscopy combined for the determination of number and spectrum of actively metabolizing bacteria in natural waters. Appl. Environ. Microbiol., 36, 506-512.
12. Mobary, B. K., Wagner, M., Urbain, V., Rittmann, B. E. and Stahl, D. A. (1996). Phylogenetic probes for analyzing abundance and spatial organization of nitrifying bacteria. Appl. Environ. Microbiol., 62, 2156-2162.
13. Nielsen, P. H., Frølund, B. and Caccavo, Jr., F. (1997). Microbial Fe(III) reduction in activated sludge. System. Appl. Microbiol., 20, 645-651.
14. Nielsen, P. H., Andreasen, K., Wagner, M., Blackall, L. L., Lemmer, H. and Seviour, R. J. (1998). Variability of type 021N in activated sludge as determined by in situ substrate uptake pattern and in situ hybridization with fluorescent rRNA-targeted probes. Wat. Sci. Tech., 37(4-5), 423-430
15. Nilsson, C. and Sundbäck, K. (1996). Amino acid uptake in natural microphytobenthic assemblages studied by microautoradiography. Hydrobiologia, 332, 119-129.
16. Novitsky, J. A. (1983). Heterotrophic activity throughout a vertical profile of seawater and sediment in Halifax Harbor, Canada. Appl. Environ. Microbiol., 45, 1753-1760.
17. Pedrós-Alió, C. and Nedwell, S. (1989). Microautoradiographic study of thymidine uptake in brackish waters around Sapelo Island, Georgia, USA. Mar. Ecol Prog. Ser., 55, 83-94.
18. Pearl, H. W. (1991). Ecophysiological and tropical implications of light-stimulated amino acid utilization in marine picoplankton. Appl. Environ. Microbiol., 57, 473-479.
19. Pearl, H. W. and Lean, D. R. S. (1976). Visual observation of phosphorus movement between algae, bacteria and abiotic particles in lake waters. J. Fish. Res. Board Can., 33, 2805-2813.
20. Rogers, A. W. (1979). Techniques of autoradiography. Elsevier, New York.
21. Roller, C., Wagner, M., Amann, R., Ludwig, W. and Schleifer, K.-H. (1994). In situ probing of gram-positive bacteria with high DNA G-C content using 23S rRNA-targeted oligonucleotides. Microbiology, 140, 2849-2858.
22. Rosselló-Mora, R. A., Wagner, M., Amann, R. and Schleifer, K.-H. (1995). The abundance of Zoogloea ramigera in sewage treatment plants. Appl. Environ. Microbiol, 61, 702-707.
23. Roszak, D. B. and Colwell, R. R. (1987). Metabolic activity of bacteria cells enumerated by direct viable count. Appl. Environ. Microbiol. 53, 2889-2983.
24. Tabor, S. P. and Neihof, R. A. (1982). Improved microautoradiographic method to determine individual microorganisms active in substrate uptake in natural waters. Appl Environ. Microbiol, 44, 945-953.
25. Tabor, S. P. and Neihof, R. A. (1984). Direct determination of activities for microorganisms of Chesapeake Bay populations. Appl Environ. Microbiol, 48, 1012-1019.
26. Wagner, M. and Amann, R. (1997). Molecular techniques for determining microbial community structures in activated sludge. In: Microbial community analysis, T. E. Cloete and N. Y. O. Muyima (eds), IAWQ Scientific and Technical Report No. 5, pp. 61-72.
27. Wagner, M., Erhart, R., Manz, W., Amann, R., Lemmer, H., Wedi, D. and Schleifer, K.-H. (1994a). Development of an rRNA-targeted oligonucleotide probe for the genus Acinetobacter and its application for in situ monitoring in activated sludge. Appl. Environ. Microbiol., 60, 792-800.
28. Wagner, M., Amann, R., Kämpfer, P., Assmus, B., Hartmann, A., Hutzler, P., Springer, N. and Schleifer, K.-H. (1994b). Identification and in situ detection of Gram-negative filamentous bacteria in activated sludge. System. Appl. Microbiol., 17, 405-417.
29. Wagner, M., Assmus, B., Hartmann, A., Hutzler, P. and Amann, R. (1994c). In situ analysis of microbial consortia in activated sludge using fluorescently labelled, rRNA-targeted oligonucleotide probes and scanning confocal laser microscopy. J. Microscopy, 176, 181-187.
30. Wagner, M., Rath, G., Amann, R., Koops, H.-P. and Schleifer, K.-H. (1995). In situ identification of ammonia-oxidizing bacteria. System. Appl. Microbiol., 18, 251-264.
31. Wagner, M., Rath, G., Koops, H.-P., Flood, J. and Amann, R. (1996). In situ analysis of nitrifying bacteria in sewage treatment plants. Wat. Sci. Tech., 34(1-2), 237-244.
32. Wagner, M., Schmid, M., Juretschko, S., Trebesius, K.-H., Bubert, A., Goebel, W. and Schleifer, K.-H. (1998). In situ detection of a virulence factor mRNA and 16S rRNA in Listeria monocytogenes. FEMS Microbiol. Lett., 160, 159-168.
33. Wanner, J. (1997). Microbial population dynamics in biological wastewater treatment plants. In: Microbial community analysis, T. E. Cloete and N. Y. O. Muyima (eds), IAWQ Scientific and Technical Report No. 5, pp. 35-59.