Methods for detection and visualization of intracellular polymers stored by polyphosphate-accumulating microorganisms

Journal of Microbiological Methods

Volumn 51, Issue 1, 2002, Pages 1-18

  Serafim, Luísa S ^a   Lemos, Paulo C ^a   Levantesi, Caterina ^b   Tandoi, Valter ^b   Santos, Helena ^c   Reis, Maria A M ^a,d  

^a DEPARTAMENTO DE QUÍMICA   (Portugal)

^b WATER RESEARCH INSTITUTE   (Italy)

^c INSTITUTO DE TECNOLOGIA QUÍMICA E BIOLÓGICA   (Portugal)

^d UNIVERSIDADE NOVA DE LISBOA   (Portugal)

Author keywords

Glycogen; NMR; Polyhydroxyalkanoates; Polyphosphate; Polyphosphate accumulating bacteria

Indexed keywords

GLYCOGEN; POLYMER; POLYPHOSPHATE;

ANALYTIC METHOD; BACTERIAL METABOLISM; MICROLUNATUS PHOSPHOVORUS; MICROSCOPY; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PRIORITY JOURNAL; QUALITATIVE ANALYSIS; REVIEW; SPIRAL AND CURVED BACTERIA; STAINING; STORAGE;

BACTERIA, AEROBIC; BACTERIA, ANAEROBIC; BIODEGRADATION, ENVIRONMENTAL; IN SITU HYBRIDIZATION, FLUORESCENCE; MAGNETIC RESONANCE SPECTROSCOPY; MICROSCOPY, FLUORESCENCE; POLYPHOSPHATES; SEWAGE;

EID: 0036277920     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-7012(02)00056-8     Document Type: Review

References (117)

1. 15N-NMR
2. The application of nuclear magnetic resonance to the study of cellular physiology
3. 2+ and energy metabolism in superfused brain slices
4. Stains for Microorganisms in Smears
5. Total phosphorus in residual materials
6. Multinuclear magnetic resonance spectroscopy of cerebral cortical slices under neuropathological conditions
7. Microbiological aspect of phosphorus removal in activated sludge system
8. Identification of some of the major groups of bacteria in efficient and nonefficient biological phosphorus removal activated sludge systems
9. A rapid gas chromatographic method for the determination of poly-β-hydroxybutyric acid in microbial diversity
10. NMR methods for measuring enzyme kinetics in vivo
11. Aerobic storage by activated sludge on real wastewater
12. A method for the determination of inorganic phosphate in the presence of labile organic phosphate and high concentrations of protein: Application to Lens ATPase
13. Isolation of intact chains of polyphosphate from Propionobacterium shermanii grown on glucose or lactate
14. A combined fluorescent antibody-membrane filter technique for enumerating Acinetobacter in activated sludges
15. Biological models for enhanced biological phosphorus removal
16. Determination of poly-β-hydroxybutyrate and poly-β-hydroxyvalerate in activated sludge by gas-liquid chromatography
17. Identification of polyphosphate-accumulating organisms and design of 16S rRNA-directed probes for their detection and quantification
18. Fluorometric measurement of poly-β-hydroxybutyrate in Alcaligenes eutrophus by flow cytometry and spectrofluorometry
19. Unreliability of cold-stored samples for assessment of chemical precipitates in activated sludge
20. An evaluation of the methods used for the determination of orthophosphate and total phosphate in activated sludge extracts
21. The use of simultaneous chemical precipitation in modified activated sludge systems exhibiting biological excess phosphate removal - Part 1. Literature review
22. Nuclear magnetic resonance studies of poly(β-hydroxybutyrate) and a copolyester of β-hydroxybutyrate and β-hydroxyvalerate isolated from Alcaligenes eutrophus H16
23. Biosynthesis and characterisation of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) in Alcaligenes eutrophus
24. NMR spectroscopy: A non-invasive tool for studying intracellular processes
25. Microbiological basis of phosphate removal in the activated sludge process for treatment of wastewater
26. Method for quantitative determination of total phosphorus in water
27. Quantification of bacterial polyhydroxyalkanoic acids by Nile red staining
28. NMR studies of intracellular metal ions in intact cells and tissues
29. A comparison of some methods for total phosphate analyses
30. A rapid method for orthophosphate analysis at high concentrations in water
31. Transmission electron microscopy of a phosphate effect on the colloid structure of iron hydroxide
32. Enrichment, phylogenetic analysis and detection of a bacterium that performs enhanced biological phosphate removal in activated sludge
33. Determination of polyhydroxyalkanoates in activated sludge by ion chromatographic and enzymatic methods
34. Anaerobic metabolism of bacteria performing enhanced biological phosphate removal
35. 13C-NMR
36. A simple method for the determination of poly-β-hydroxybutyric acid in microbial biomass
37. Characterization of bacterial communities from activated sludge: Culture-dependent numerical identification versus in situ identification using group- and genus-specific rRNA-targeted oligonucleotides probes
38. Analysis of poly-β-hydroxybutyrate in Rhizobium japonicum bacteroids by ion-exclusion high-pressure liquid chromatography and UV detection
39. In situ identification of polyphosphate-accumulating bacteria in activated sludges by dual staining with r-RNA targeted oligonucleotide probes (DAPI) at a polyphosphate-probing concentration
40. Flow cytometric sorting and RFLP analysis of phosphate accumulating bacteria in an enhanced biological phosphorus removal system
41. 13C-NMR study of Dictyostelium discodeum metabolism
42. Microbial production of poly-β-hydroxybutyric acid
43. Assay of poly-β-hydroxybutyric acid
44. Combination of fluorescent in situ hybridisation and microautoradiography a new tool for structure-function analyses in microbial ecology
45. Products of dehydration and of polymerization of β-hydroxybutyric acid
46. Effect of carbon source on the formation of polyhydroxyalkanoates (PHA) by a phosphate-accumulating mixed culture
47. The distribution and movement of polyphosphate and associated cations in sludges from NDEBPR plants in different configurations at pilot scale
48. Practical methods for the examination and characterization of activated sludge
49. Glycogen accumulating population and its anaerobic substrate uptake in anaerobic-aerobic activated sludge without biological phosphorus removal
50. In situ identification of polyphosphate- and polyhydroxyalkanoate-accumulating traits for microbial population in a biological phosphorus removal process
51. The distribution of phosphorus ions in the phosphorus removal process
52. The role of bacterial phosphate metabolism in enhanced phosphorus removal from the activated sludge process
53. Microscopic evaluation of carbon and phosphorus accumulation in nutrient removal activated sludge plants
54. 13C nuclear magnetic resonance
55. Nuclear magnetic resonance studies of cellular metabolism
56. Influence of storage on kinetic selection to control aerobic filamentous bulking
57. Comparison of carbon storage under aerobic and anoxic conditions
58. Intracellular carbon flow in phosphorus accumulating organisms from activated sludge systems
59. Location of phosphorus in activated sludge and function of intracellular polyphosphates in biological phosphorus removal process
60. Effect of phosphorus accumulation on acetate metabolism in the biological phosphate removal process
61. Microbiology and biochemistry of the enhanced biological phosphate removal process
62. A modified single solution method for the determination of phosphate in natural waters
63. Determinative and cytological light microscopy
64. Isolation and analysis of intact polyphosphate chains from activated sludges associated with biological phosphate removal
65. Microlunatus phosphovorus gentile, nov., sp. nov., a new Gram-positive polyphosphate accumulating bacterium isolated from activated sludge
66. 13C-NMR
67. 31P Nuclear magnetic resonance studies of energy transduction in Rhodopseudomonas sphaeroides
68. Analysis of microbial community that performs enhanced biological phosphorus removal in activated sludge fed with acetate
69. SEM-EDS for determining the phosphorus content in activated sludge EPS
70. Nile blue as a fluorescent stain for poly-β-hydroxybutyrate
71. 13C-NMR labelling experiments
72. Methods for phosphorus analysis for soils, sediments, residuals and waters: Introduction
73. Fractionation of phosphorus in suspended matter and sediment
74. On the enzymatic determination of blood glucose
75. Differentiation of polyphosphate and poly-β-hydroxybutyrate granules in Acinetobacter sp. isolated from activated sludge
76. Gas chromatographic determination of poly-β-hydroxybutyric acid in microbial biomass after hydrochloric propanolysis
77. Monitoring of cellular metabolism by NMR
78. Nuclear Magnetic Resonance
79. Polyphosphates
80. Interactions between chemical and advanced biological phosphorus elimination
81. Assay of inorganic phosphate in the mild pH range, suitable for measurement of glycogen phosphorylase activity
82. In vivo Nuclear Magnetic Resonance in study of physiology of sulphate reducing bacteria
83. Solutes from thermofiles and hyperthermophiles
84. Glucose metabolism and kinetics of phosphorus removal by fermentative bacterium Microlunatus phosphovorus
85. Uptake of organic substrates and accumulation of polyhydroxyalkanoates linked with glycolysis of intracellular carbohydrates under anaerobic conditions in the biological excess phosphate removal processes
86. Microbiology of 'G-bacteria' in activated sludge
87. Micropruina glycogenica gen nov., sp. nov., a new Gram-positive glycogen-accumulating bacterium isolated from activated sludge
88. 31P-NMR study
89. 31P nuclear magnetic resonance of methylphosphonate and phosphate
90. Model of the anaerobic metabolism of the biological phosphorus removal process: Stoichiometry and pH influence
91. A sensitive, viable-colony staining method using Nile red for direct screening of bacteria that accumulate polyhydroxyalcanoic acids and other lipid storage compounds
92. Measurements of metal cation compartmentalization in tissue by high-resolution metal cation NMR
93. Biological phosphorus removal by pure culture Lampropedia spp.
94. Polyphosphate-accumulating bacteria from sewage plant with different processes of biological phosphorus removal
95. Synthesis, structure and properties of polyhydroxyalkanoates: Biological polyesters
96. The behaviour or polyphosphate accumulating Acinetobacter isolates in an aerobic chemostat
97. 13C-NMR studies of polymer synthesis in Rhizobium meliloti M5N1 strain
98. Phylogenetic and physiological diversity of tetrad-forming organisms in deteriorated biological phosphorus removal system
99. A simple method to distinguish between polyphosphate and other fractions of activated sludge
100. Structures and properties of condensed phosphates: I. Some general considerations about phosphoric acids
101. Development of an rRNA-targeted oligonucleotide probe specific for the genus Acinetobacter and its application for in situ monitoring in activated sludge
102. Metabolic behaviour of Acinetobacter spp. in biological enhanced phosphorus removal - A biochemical model
103. The isolation and estimation of poly-β-hydroxybutyrate inclusions of Bacillus species
104. 19F NMR studies of fluorine-labelled yeast phosphoglycerate kinase in vivo
105. 13C-labeling experiments and NMR analysis
106. Presence of Rhodocyclus in a full-scale wastewater treatment plant and their participation in enhanced biological phosphorus removal