The histone-like nucleoid structuring protein H-NS represses the Escherichia coli bgl operon downstream of the promoter

Molecular Microbiology

Volumn 52, Issue 2, 2004, Pages 589-600

  Dole, Sudhanshu ^a,b   Nagarajavel, V ^a   Schnetz, Karin ^a  

^a UNIVERSITY OF COLOGNE   (Germany)

^b HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ELONGATION FACTOR; HISTONE; MESSENGER RNA; RNA POLYMERASE; TRANSCRIPTION TERMINATION FACTOR RHO;

ARTICLE; ENZYME ACTIVE SITE; ENZYME SPECIFICITY; ESCHERICHIA COLI; GENE CONTROL; GENE EXPRESSION REGULATION; GENE LOCATION; GENE REPRESSION; GENE SILENCING; LACTOSE OPERON; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN BINDING; PROTEIN FOLDING; PROTEIN STRUCTURE; TRANSLATION INITIATION;

BACTERIAL PROTEINS; DNA-BINDING PROTEINS; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; GLUCOSIDES; MODELS, GENETIC; OPERON; PROMOTER REGIONS (GENETICS); TRANSCRIPTION, GENETIC;

ESCHERICHIA COLI; NEGIBACTERIA; TRIXIS;

EID: 1942532920     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1365-2958.2004.04001.x     Document Type: Article

References (50)

1. Amit, R., Oppenheim, A.B., and Stavans, J. (2003) Increased bending rigidity of single DNA molecules by H-NS, a temperature and osmolarity sensor. Biophys J 84: 2467-2473.
2. Badaut, C., Williams, R., Arluison, V., Bouffartigues, E., Robert, B., Buc, H., and Rimsky, S. (2002) The degree of oligomerization of the H-NS nucleoid structuring protein is related to specific binding to DNA. J Biol Chem 277: 41657-41666.
3. Beloin, C., and Dorman, C.J. (2003) An extended role for the nucleoid structuring protein H-NS in the virulence gene regulatory cascade of Shigella flexneri. Mol Microbiol 47: 825-838.
4. Bloch, V., Yang, Y., Margeat, E., Chavanieu, A., Auge, M.T., Robert, B., et al. (2003) The H-NS dimerization domain defines a new fold contributing to DNA recognition. Nat Struct Biol 10: 212-218.
5. Caramel, A., and Schnetz, K. (1998) Lac and Lambda represser relieve silencing of the Escherichia coli bgl promoter. Activation by alteration of a repressing nucleoprotein complex. J Mol Biol 284: 875-883.
6. Carter, T., and Newton, A. (1969) Messenger RNA stability and polarity in the lac operon of Escherchia coli. Nature 223: 707-710.
7. Chang, A.C.Y., and Cohen, S.N. (1978) Construction and charcterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J Bacteriol 134: 1141-1156.
8. Colonna, B., Casalino, M., Fradiani, P.A., Zagaglia, C., Naitza, S., Leoni, L., et al. (1995) H-NS regulation of virulence gene expression in enteroinvasive Escherichia coli harboring the virulence plasmid integrated into the host chromosome. J Bacteriol 177: 4703-4712.
9. Dame, R.T., Wyman, C., and Goosen, N. (2000) H-NS mediated compaction of DNA visualised by atomic force microscopy. Nucleic Acids Res 28: 3504-3510.
10. Dame, R.T., Wyman, C., Wurm, R., Wagner, R., and Goosen, N. (2002) Structural basis for H-NS-mediated trapping of RNA polymerase in the open initiation complex at the rrnB P1. J Biol Chem 277: 2146-2150.
11. Dattananda, C.S., Rajkumari, K., and Gowrishankar, J. (1991) Multiple mechanims contribute to osmotic inducibility of proU operon expression in Escherichia coli: demonstration of two osmoresponsive promoters and of a negative regulatory element within the first structural gene. J Bacteriol 173: 7481-7490.
12. Defez, R., and de Felice, M. (1981) Cryptic operon for β-glucoside metabolism in Escherichia coli K12: Genetic evidence for a regulatory protein. Genetics 97: 11-25.
13. Dersch, P., Schmidt, K., and Bremer, E. (1993) Synthesis of the Escherichia coli K-12 nucleoid-associated DNA-binding protein H-NS is subjected to growth-phase control and autoregulation. Mol Microbiol 8: 875-889.
14. Diederich, L., Rasmussen, L.J., and Messer, W. (1992) New cloning vectors for integration into the lambda attachment site attB of the Escherichia coli chromosome. Plasmid 28: 14-24.
15. Dole, S., Kühn, S., and Schnetz, K. (2002) Post-transcriptional enhancement of Escherichia coli bgloperon silencing by limitation of BglG-mediated antitermination at low transcription rates. Mol Microbiol 43: 217-226.
16. Epshtein, V., Toulme, F., Rahmouni, A.R., Borukhov, S., and Nudler, E. (2003) Transcription through the roadblocks: the role of RNA polymerase cooperation. EMBO J 22: 4719-4727.
17. Esposito, D., Petrovic, A., Harris, R., Ono, S., Eccleston, J.F., Mbabaali, A., et al. (2002) H-NS oligomerization domain structure reveals the mechanism for high order self association of the intact protein. J Mol Biol 324: 841-850.
18. Falconi, M., Brandi, A., La Teana, A., Gualerzi, C.O., and Pon, C.L. (1996) Antagonistic involvement of FIS and H-NS proteins in the transcriptional control of hns expression. Mol Microbiol 19: 965-975.
19. Görke, B., and Rak, B. (1999) Catabolite control of Escherichia coli regulatory protein BglG activity by antagonistically acting phosphorylations. EMBO J 18: 3370-3379.
20. Higgins, C.F., Dorman, C.J., Stirling, D.A., Waddell, L., Booth, I.R., May, G., and Bremer, E. (1988) A physiological role for DNA supercolling in the osmotic regulation of gene expression in S. typhimurium and E coli. Cell 52: 569-584.
21. Hommais, F., Krin, E., Laurent-Winter, C., Soutourina, O., Malpertuy, A., Le Caer, J.P., et al. (2001) Large-scale monitoring of pleiotropic regulation of gene expression by the prokaryotic nucleoid-associated protein, H-NS. Mol Microbiol 40: 20-36.
22. Jordi, B.J., and Higgins, C.F. (2000) The downstream regulatory element of the proU operon of Salmonella typhimurium inhibits open complex formation by RNA polymerase at a distance. J Biol Chem 275: 12123-12128.
23. Jordi, B.J.A.M., Dagberg, B., de Haan, L.A.M., Hamers, A.M., van der Zeijst, B.A.M., Gaastra, W., and Uhlin, B.E. (1992) The positive regulator CfaD overcomes the repression mediated by histone-like protein H-NS (H1) in the CFA/I fimbrial operon of Escherichia coli. EMBO J 11: 2627-2632.
24. Jordi, B.J.A.M., Fielder, A.E., Burns, C.M., Hinton, J.C.D., Dover, N., Ussery, D.W., and Higgins, C.F. (1997) DNA binding is not sufficient for H-NS mediated repression of proU expression. J Biol Chem 272: 12083-12090.
25. Landick, R. (2001) Rna polymerase clamps down. Cell 105: 567-570.
26. Lopilato, J., and Wright, A. (1990) Mechanisms of activation of the cryptic bgl operon of Escherichia coli K-12. In The Bacterial Chromosome. Drlica, K., and Riley, M. (eds). Washington, DC: American Society for Microbiology, pp. 435-444.
27. Lucht, J.M., Dersch, P., Kempf, B., and Bremer, E. (1994) Interactions of the Nucloid-associated DNA-binding protein H-NS with the regulatory region of the osmotically controlled proU operon of Escherichia coli. J Biol Chem 269: 6578-6586.
28. Miller, J.H. (1972) Experiments in Molecular Genetics. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory.
29. Mukerji, M., and Mahadevan, S. (1997) Characterization of the negative elements involved in silencing the bgl operon of Escherichia coli: possible roles for DNA gyrase, H-NS, and CRP-cAMP in regulation. Mol Microbiol 24: 617-627.
30. Munteanu, M.G., Vlahovicek, K., Parthasarathy, S., Simon, I., and Pongor, S. (1998) Rod models of DNA: sequence-dependent anisotropic elastic modelling of local bending phenomena. Trends Biochem Sci 23: 341-347.
31. Overdier, D.G., and Csonka, L.N. (1992) A transcriptional silencer downstream of the promoter in the osmotically controlled proU operon of Salmonella typhimurium. Proc Natl Acad Sci USA 89: 3140-3144.
32. Owen-Hughes, T.A., Pavitt, G.D., Santos, D.S., Sidebotham, J.M., Hulton, C.S.J., Hinton, J.C.D., and Higgins, C.F. (1992) The chromatin-associated protein H-NS interacts with curved DNA to influence DNA topology and gene expression. Cell 71: 255-265.
33. Prentki, P., and Krisch, H.M. (1984) In vitro insertional mutagenesis with a selectable DNA fragment. Gene 29: 303-313.
34. Reynolds, A.E., Felton, J., and Wright, A. (1981) Insertion of DNA activates the cryptic bgl operon of E. coli K12. Nature 293: 625-629.
35. Reynolds, A.E., Mahadevan, S., LeGrice, S.F.J., and Wright, A. (1986) Enhancement of bacterial gene expression by insertion elements or by mutation in a CAP-cAMP binding site. J Mol Biol 191: 85-95.
36. Rimsky, S., Zuber, F., Buckle, M., and Buc, H. (2001) A molecular mechanism for the repression of transcription by the H-NS protein. Mol Microbiol 42: 1311-1323.
37. Sambrook, J., and Russell, D. (2001) Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
38. Schaefler, S., and Maas, W.K. (1967) Inducible system for the utilization of β-glucosides in Escherichia coli II. Description of mutant types and genetic analysis. J Bacteriol 93: 264-272.
39. Schnetz, K. (1995) Silencing of Escherichia coli bgl promoter by flanking sequence elements. EMBO J 14: 2545-2550.
40. Schnetz, K. (2002) Silencing of the Escherichia coli bgl operon by RpoS requires CrI. Microbiology 148: 2573-2578.
41. Schnetz, K., and Rak, B. (1992) IS5: a mobile enhancer of transcription in Escherichia coli. Proc Natl Acad Sci USA 89: 1244-1248.
42. Schnetz, K., and Wang, J.C. (1996) Silencing of Escherichia coli bgl promoter: Effects of template supercoiling and cell extracts on promoter activity in vitro. Nucleic Acids Res 24: 2422-2429.
43. Schnetz, K., Toloczyki, C., and Rak, B. (1987) β-Glucoside (bgl) operon of Escherichia coli K-12: nucleotide sequence, genetic organization, and possible evolutionary relationship to regulatory components of two Bacillus subtilis genes. J Bacteriol 169: 2579-2590.
44. Schnetz, K., Sutrina, S.L., Saier, M.H. Jr and Rak, B. (1990) Identification of catalytic residues in the β-glucoside permease of Escherichia coli by site-specific mutagenesis and demonstration of interdomain cross reactivity between the β-glucoside and glucose systems. J Biol Chem 265: 13464-13471.
45. Schröder, O., and Wagner, R. (2002) The bacterial regulatory protein H-NS - a versatile modulator of nucleic acid structures. Biol Chem 383: 945-960.
46. Severinov, K. (2000) RNA polymerase structure-function: insights into points of transcriptional regulation. Curr Opin Microbiol 3: 118-125.
47. Shindo, H., Ohnuki, A., Ginba, H., Katoh, E., Ueguchi, C., Mizuno, T., and Yamazaki, T. (1999) Identification of the DNA binding surface of H-NS protein from Escherichia coli by heteronuclear NMR spectroscopy. FEBS Lett 455: 63-69.
48. Ussery, D.W., Hinton, J.C.D., Jordi, B.J.A.M., Granum, P.E., Seirafi, A., Stephen, R.J., et al. (1994) The chromatin-associated protein H-NS. Biochimie 76: 968-980.
49. Wilson, G.G., Young, K.Y.K., Edlin, G.J., and Konigsberg, W. (1979) High-frequency generalised transduction by bacteriophage T4. Nature 280: 80-82.
50. Zhang, A., Rimsky, S., Reaban, M.E., Buc, H., and Belfort, M. (1996) Escherichia coli protein analogs StpA and H-NS: regulatory loops, similar and disparate effects on nucleic acis dynamics. EMBO J 15: 1340-1349.