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Volumn 304, Issue 5669, 2004, Pages 428-431

A Functional Protein Chip for Pathway Optimization and in Vitro Metabolic Engineering

Author keywords

[No Author keywords available]

Indexed keywords

CATALYSIS; DNA; ENZYMES; GENES; METABOLITES; OPTIMIZATION; RNA;

EID: 1942520229     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1096920     Document Type: Article
Times cited : (58)

References (24)
  • 11
  • 12
    • 1942486491 scopus 로고    scopus 로고
    • note
    • Materials and methods are available as supporting material on Science Online.
  • 17
    • 1942453952 scopus 로고    scopus 로고
    • note
    • We did not actually measure the activities of the five enzymes; their actual amounts and activities were controlled by the amount of the corresponding homologous capture DNA that was spotted on the well of the 96-well microplate.
  • 18
    • 1942486490 scopus 로고    scopus 로고
    • note
    • Capture DNA molecules for each enzyme used in this study are as follows: udpgp, 5′-GCGCTGCT-CAAGGAGTGATT-3′; hxk1, 5′ -CAACTTTTC-GATATCGGAAA-3′; pgm, 5′-CGTCTTTATCAAG-GTGCATA-3′; otsA, 5′-CAAGAACGCCCGGATTG-GCT-3′; otsB, 5′ -TCACCTTTACTGGTACCTCT-3′. Trehalose synthesis was carried out in the buffer containing 50 mM tris-HCl (pH 7.6), 10 mM magnesium chloride, 110 mM glucose, 55 mM adenosine 5′-triphosphate (ATP), 16.7 mM uridine 5′-triphosphate, and 16.7 μg of glucose-1,6-bisphosphate per milliliter of solution for 3 hours at 30°C. Synthesized trehalose was measured using the method for acid trehalase (23).
  • 24
    • 1942422330 scopus 로고    scopus 로고
    • note
    • We acknowledge support from the DuPont-MIT Alliance and the Singapore MIT Alliance.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.