Regulation of homologous integration in yeast by the DNA repair proteins Ku70 and RecQ

Molecular Genetics and Genomics

Volumn 273, Issue 2, 2005, Pages 167-176

  Yamana, Yoshimasa ^a   Maeda, Toshinari ^a   Ohba, Hiroyuki ^a   Usui, Takehiko ^b   Ogawa, Hiroaki I ^a   Kusano, Kohji ^a  

^a KYUSHU INSTITUTE OF TECHNOLOGY   (Japan)

^b MEMORIAL SLOAN KETTERING CANCER CENTER   (United States)

Author keywords

Double strand break repair; Ku70; Sgs1; Targeted integration

Indexed keywords

CAMPTOTHECIN; DOUBLE STRANDED DNA; HELICASE; KU ANTIGEN; MUTANT PROTEIN; POLYDEOXYRIBONUCLEOTIDE SYNTHASE; RAD51 PROTEIN; RECQ HELICASE; SLOW GROWTH SUPPRESSOR 1 PROTEIN; TYPE II SITE SPECIFIC DEOXYRIBONUCLEASE; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; DNA STRAND BREAKAGE; EUKARYOTE; GENE CONTROL; GENE DELETION; GENE EXPRESSION; GENE FREQUENCY; GENE INACTIVATION; GENE INTERACTION; GENE MUTATION; GENE REPRESSION; GENE TARGETING; GENETIC CODE; HOMOLOGOUS RECOMBINATION; MODULATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; SACCHAROMYCES CEREVISIAE; SENSITIVITY ANALYSIS; SEQUENCE HOMOLOGY; YEAST;

CAMPTOTHECIN; DNA HELICASES; DNA PRIMERS; DNA REPAIR; DNA-BINDING PROTEINS; GENE TARGETING; GENOMIC INSTABILITY; MUTATION; PLASMIDS; RECOMBINATION, GENETIC; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

ESCHERICHIA COLI; EUKARYOTA; SACCHAROMYCES CEREVISIAE;

EID: 18744398757     PISSN: 16174615     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00438-005-1108-y     Document Type: Article

References (48)

1. Adams MD, McVey M, Sekelsky JJ (2003) Drosophila BLM in double-strand break repair by synthesis-dependent strand annealing. Science 299:265-267
2. Barnes G, Rine J (1985) Regulated expression of endonuclease EcoRI in Saccharomyces cerevisiae: nuclear entry and biological consequences. Proc Natl Acad Sci USA 82:1354-1358
3. Boulton SJ, Jackson SP (1996a) Identification of a Saccharomyces cerevisiae Ku80 homologue: roles in DNA double strand break rejoining and in telomeric maintenance. Nucleic Acids Res 24:4639-4648
4. Boulton SJ, Jackson SP (1996b) Saccharomyces cerevisiae Ku70 potentiates illegitimate DNA double-strand break repair and serves as a barrier to error-prone DNA repair pathways. EMBO J 15:5093-5103
5. Capozzo C, Sartorello F, Pero FD, D'Angelo M, Vezzi A, Campanaro S, Valle G (2000) Gene disruption and basic phenotypic analysis of nine novel yeast genes from chromosome XIV. Yeast 16:1089-1097
6. Feldmann H, Winnacker EL (1993) A putative homologue of the human autoantigen Ku from Saccharomyces cerevisiae. J Biol Chem 268:12895-12900
7. Ferguson DO, Holloman WK (1996) Recombinational repair of gaps in DNA is asymmetric in Ustilago maydis and can be explained by a migrating D-loop model. Proc Natl Acad Sci USA 93:5419-5424
8. Gietz RD, Schiestl RH, Willems AR, Woods RA (1995) Studies on the transformation of intact yeast cells by the LiAc/SS-DNA/PEG procedure. Yeast 11:355-360
9. Goedecke W, Eijpe M, Offenberg HH, van Aalderen M, Heyting C (1999) Mre11 and Ku70 interact in somatic cells, but are differentially expressed in early meiosis. Nat Genet 23:194-198
10. Hashida-Okado T, Ogawa A, Kato I, Takesako K (1998) Transformation system for prototrophic industrial yeasts using the AUR1 gene as a dominant selection marker. FEBS Lett 425:117-122
11. Hastings PJ, McGill C, Shafer B, Strathern JN (1993) Ends-in vs. ends-out recombination in yeast. Genetics 135:973-980
12. Holmes AM, Haber JE (1999) Double-strand break repair in yeast requires both leading and lagging strand DNA polymerases. Cell 96:415-424
13. Hsiang YH, Hertzberg R, Hecht S, Liu LF (1985) Camptothecin induces protein-linked DNA breaks via mammalian DNA topoisomerase I. J Biol Chem 260:14873-14878
14. Ira G, Haber JE (2002) Characterization of RAD51-independent break-induced replication that acts preferentially with short homologous sequences. Mol Cell Biol 22:6384-6392
15. Ira G, Malkova A, Liberi G, Foiani M, Haber JE (2003) Srs2 and Sgs1-Top3 suppress crossovers during double-strand break re-pair in yeast. Cell 115:401-411
16. Kang LE, Symington LS (2000) Aberrant double-strand break repair in rad51 mutants of Saccharomyces cerevisiae. Mol Cell Biol 20:9162-9172
17. Kooistra R, Pastink A, Zonneveld JB, Lohman PH, Eeken JC (1999) The Drosophila melanogaster DmRAD54 gene plays a crucial role in double-strand break repair after P-element excision and acts synergistically with Ku70 in the repair of X-ray damage. Mol Cell Biol 19:6269-6275
18. Kusano K (2004) Cell death promoted by homologous DNA interaction from bacteria to humans. In: Ebashi S, Noda H (eds) Novel developments on genetic recombination: DNA double-strand break and DNA end-joining. Japan Scientific Societies Press, Tokyo, pp 81-96
19. Kusano K, Sunohara Y, Takahashi N, Yoshikura H, Kobayashi I (1994) DNA double-strand break repair: genetic determinants of flanking crossing-over. Proc Natl Acad Sci USA 91:1173-1177
20. Kusano K, Berres ME, Engels WR (1999) Evolution of the RECQ family of hlicases: a Drosophila homolog of RECQ helicase, Dmblm, is similar to the human Bloom syndrome gene. Genetics 151:1027-1039
21. Kusano K, Johnson-Schlitz DM, Engels WR (2001) Sterility of Drosophila with mutations in the Bloom syndrome gene: complementation by Ku70. Science 291:2600-2602
22. Lewis LK, Kirchner JM, Resnick MA (1998) Requirement for end-joining and checkpoint functions, but not RAD52-mediated recombination, after EcoRI endonuclease cleavage of Saccharomyces cerevisiae DNA. Mol Cell Biol 18:1891-1902
23. Lewis LK, Karthikeyan G, Westmoreland JW, Resnick MA (2002) Differential suppression of DNA repair deficiencies of yeast rad50, mre11 and xrs2 mutants by EXO1 and TLC1 (the RNA component of telomerase). Genetics 160:49-62
24. Luo G, Santoro I, McDaniel L, Nishijima I, Mills M, Youssoufian H, Vogel H, Schultz R, Bradley A (2000) Cancer predisposition caused by elevated mitotic recombination in Bloom mice. Nat Genet 26:424-429
25. McGill C, Shafer B, Strathern J (1989) Coconversion of flanking sequences with homothallic switching. Cell 57:459-467
26. Mueller JE, Clyman J, Huang Y-J, Parker MM, Belfort M (1996) Intron mobility in phage T4 occurs in the context of recombination-dependent DNA replication by way of multiple pathways. Genes Dev 10:351-364
27. Nakayama K, Kusano K, Irino N, Nakayama H (1994) Thymine starvation-induced structural changes in Escherichia coli DNA: detection by pulsed field gel electrophoresis and evidence for involvement of homologous recombination. J Mol Biol 243:611-620
28. Nassif N, Penney J, Pal S, Engels WR, Gloor GB (1994) Efficient copying of nonhomologous sequences from ectopic sites via P-element-induced gap repair. Mol Cell Biol 14:1613-1625
29. Nelson HH, Sweetser DB, Nickoloff JA (1996) Effects of terminal nonhomology and homeology on double-strand-break-induced gene conversion tract directionality. Mol Cell Biol 16:2951-2957
30. Onoda F, Seki M, Miyajima A, Enomoto T (2001) Involvement of SGS1 in DNA damage-induced heteroallelic recombination that requires RAD52 in Saccharomyces cerevisiae. Mol Gen Genet 264:702-708
31. Orr-Weaver TL, Szostak JW, Rothstein RJ (1983) Genetic applications of yeast transformation with linear and gapped plasmids. Methods Enzymol 101:228-245
32. Paques F, Leung W-Y, Haber JE (1998) Expansion and contractions in a tandem repeat induced by double-strand break repair. Mol Cell Biol 18:2045-2054
33. Pierce AJ, Hu P, Han M, Ellis N, Jasin M (2001) Ku DNA end-binding protein modulates homologous repair of double-strand breaks in mammalian cells. Genes Dev 15:3237-3242
34. Ramos W, Liu G, Giroux CN, Tomkinson AE (1998) Biochemical and genetic characterization of the DNA ligase encoded by Saccharomyces cerevisiae open reading frame YOR005c, a homolog of mammalian DNA ligase IV. Nucleic Acids Res 26:5676-5683
35. Resnick MA, Martin P (1976) The repair of double-strand breaks in the nuclear DNA of Saccharomyces cerevisiae and its genetic control. Mol Gen Genet 143:119-129
36. Rothstein RJ (1983) One-step gene disruption in yeast. Methods Enzymol 101:201-211
37. Rothstein RJ (1991) Targeting, disruption, replacement, and allele rescue: integrative DNA transformation in yeast. Methods Enzymol 194:281-301
38. Schar P, Herrmann G, Daly G, Lindahl T (1997) A newly identified DNA ligase of Saccharomyces cerevisiae involved in RAD52-independent repair of DNA double-strand breaks. Genes Dev 11:1912-1924
39. Sikorski RS, Hieter P (1989) A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122:19-27
40. Strumberg D, Pilon AA, Smith M, Hickey R, Malkas L, Pommier Y (2000) Conversion of topoisomerase I cleavage complexes on the leading strand of ribosomal DNA into 5′-phosphorylated DNA double-strand breaks by replication runoff. Mol Cell Biol 20:3977-3987
41. Szostak JW, Orr-Weaver TL, Rothstein RJ, Stahl FW (1983) The double-strand-break repair model for recombination. Cell 33:25-35
42. Thomas BJ, Rothstein R (1989) Elevated recombination rates in transcriptionally active DNA. Cell 56:619-630
43. Valencia M, Bentele M, Vaze M, Herrmann G, Kraus E, Lee SE, Schar P, Haber JE (2001) NEJ1 controls non-homologous end joining in Saccharomyces cerevisiae. Nature 414:666-669
44. Van Brabant AJ, Ye T, Sanz M, German III JL, Ellis NA, Holloman WK (2000) Binding and melting of D-loops by the Bloom syndrome helicase. Biochemistry 39:14617-14625
45. Wang W, Seki M, Narita Y, Sonoda E, Takeda S, Yamada K, Masuko T, Katada T, Enomoto T (2000) Possible association of BLM in decreasing DNA double strand breaks during DNA replication. EMBO J 19:3428-3435
46. Watt PM, Hickson ID, Sorts RH, Louis EJ (1996) SGS1, a homologue of the Bloom's and Werner's syndrome genes, is required for maintenance of genomic stability in Saccharomyces cerevisiae. Genetics 144:935-945
47. Wu L, Hickson ID (2003) The Bloom's syndrome helicase suppresses crossing over during homologous recombination. Nature 426:870-874
48. Yamagata K, Kato J, Shimamoto A, Goto M, Furuichi Y, Ikeda H (1998) Bloom's and Werner's syndrome genes suppress hyperrecombination in yeast sgs1 mutant: implication for genomic instability in human diseases. Proc Natl Acad Sci USA 95:8733-8738