Enhanced green fluorescent protein expression may be used to monitor murine coronavirus spread in vitro and in the mouse central nervous system

Journal of NeuroVirology

Volumn 8, Issue 5, 2002, Pages 381-391

  Das Sarma, Jayasri ^a   Scheen, Esther ^a   Seo, Su Hun ^a   Koval, Michael ^a   Weiss, Susan R ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

Murine coronavirus; Viral genetics; Viral pathogenesis

Indexed keywords

GREEN FLUORESCENT PROTEIN; MESSENGER RNA; VIRUS ANTIGEN; MEMBRANE PROTEIN; PHOTOPROTEIN; RECOMBINANT PROTEIN; SPIKE GLYCOPROTEIN, CORONAVIRUS; VIRUS ENVELOPE PROTEIN;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CELL LYSATE; CENTRAL NERVOUS SYSTEM INFECTION; CONTROLLED STUDY; DEMYELINATION; ELECTROPHORETIC MOBILITY; ENCEPHALITIS; FLUORESCENCE MICROSCOPY; GENE EXPRESSION; GENE INSERTION; IN VITRO STUDY; MALE; MONITORING; MURINE HEPATITIS CORONAVIRUS; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; TISSUE CULTURE; VIRUS ATTENUATION; VIRUS GENOME; VIRUS ISOLATION; VIRUS PATHOGENESIS; VIRUS PLAQUE; VIRUS RECOMBINATION; VIRUS REPLICATION; VIRUS STRAIN; VIRUS VIRULENCE; WESTERN BLOTTING; WILD TYPE; ANIMAL; BIOSYNTHESIS; BRAIN; C57BL MOUSE; CAT; CENTRAL NERVOUS SYSTEM; COMPARATIVE STUDY; DISEASE MODEL; GENETIC REASSORTMENT; GENETIC RECOMBINATION; GENETICS; L CELL; MOUSE; PATHOGENICITY; VIROLOGY; VIRULENCE; VIRUS CULTURE; VIRUS INFECTION;

ANIMALIA; CORONAVIRUS; MURINAE; MURINE HEPATITIS VIRUS; MURINE HEPATITIS VIRUS STRAIN 4;

ANIMALS; ANTIGENS, VIRAL; BRAIN; CATS; CENTRAL NERVOUS SYSTEM; CORONAVIRUS INFECTIONS; DISEASE MODELS, ANIMAL; GREEN FLUORESCENT PROTEINS; L CELLS (CELL LINE); LUMINESCENT PROTEINS; MALE; MEMBRANE GLYCOPROTEINS; MICE; MICE, INBRED C57BL; MURINE HEPATITIS VIRUS; REASSORTANT VIRUSES; RECOMBINANT PROTEINS; RECOMBINATION, GENETIC; RNA, MESSENGER; SERIAL PASSAGE; VIRAL ENVELOPE PROTEINS; VIRULENCE; VIRUS REPLICATION;

EID: 1842859786     PISSN: 13550284     EISSN: None     Source Type: Journal    
DOI: 10.1080/13550280260422686     Document Type: Article

References (25)

1. Bond CW, Leibowitz JL, Robb JA (1979). Pathogenic murine coronaviruses. II. Characterization of virus-specific proteins of murine coronaviruses JHMV and A59V. Virology 94: 371-384.
2. Cormack BP, Valdivia RH, Falkow S (1996). FACS-optimized mutants of the green fluorescent protein (GFP). Gene 173: 33-38.
3. Das Sarma J, Fu L, Tsai JC, Weiss SR, Lavi E (2000). Demyelination determinants map to the spike glycoprotein gene of coronavirus mouse hepatitis virus. J Virol 74: 9206-9213.
4. Fischer F, Stegen CF, Koetzner CA, Masters PS (1997). Analysis of a recombinant mouse hepatitis virus expressing a foreign gene reveals a novel aspect of coronavirus transcription. J Virol 71: 5148-5160.
5. Gombold JL, Hingley ST, Weiss SR (1993). Fusion-defective mutants of mouse hepatitis virus A59 contain a mutation in the spike protein cleavage signal. J Virol 67: 4504-4512.
6. Gombold JL, Weiss SR (1992). Mouse hepatitis virus A59 increases steady state levels of MHC mRNAs in primary glial cell cultures and in the murine central nervous system. Microb Pathogen 13: 493-505.
7. Haas J, Park EC, Seed B (1996). Codon usage limitation in the expression of HIV-1 envelope glycoprotein. Curr Biol 6: 315-324.
8. Houtman JJ, Fleming JO (1996). Dissociation of demyelination and viral clearance in congenitally immunodeficient mice infected with murine coronavirus JHM. J NeuroVirol 2: 101-110.
9. Kuo L, Godeke GJ, Raamsman MJ, Masters PS, Rottier PJ (2000). Retargeting of coronavirus by substitution of the spike glycoprotein ectodomain: Crossing the host cell species barrier. J Virol 74: 1393-1406.
10. Lavi E, Gilden DH, Wroblewska Z, Rorke LB, Weiss SR (1984). Experimental demyelination produced by the A59 strain of mouse hepatitis virus. Neurology 34: 597-603.
11. Lavi E, Murray EM, Makino S, Stohlman SA, Lai MMC, Weiss SR (1990). Determinants of coronavirus MHV pathogenesis are localized to 3′ portions of the genome as determined by ribonucleic acid-ribonucleic acid recombination. Lab Invest 62: 570-578.
12. Leparc-Goffart I, Hingley ST, Chua MM, Jiang X, Lavi E, Weiss SR (1997). Altered pathogenesis of a mutant of the murine coronavirus MHV-A59 is associated with a Q159L amino acid substitution in the spike protein. Virology 239: 1-10.
13. Leparc-Goffart I, Hingley ST, Chua MM, Phillips J, Lavi E, Weiss SR (1998). Targeted recombination within the spike gene of murine coronavirus mouse hepatitis virus-A59: Q159 is a determinant of hepatotropism. J Virol 72: 9628-9636.
14. Luytjes W, Bredenbeek PJ, Noten AF, Horzinek MC, Spaan, WJM (1988). Sequence of mouse hepatitis virus A59 mRNA 2: indications for RNA recombination between coronaviruses and influenza C virus. Virology 166: 415-422.
15. Matthews AE, Weiss SR, Shlomchik MJ, Hannum LG, Gombold JL, Paterson Y (2001). Antibody is required for clearance of infectious murine hepatitis virus A59 from the central nervous system, but not the liver. J Immunol 167: 5254-5263.
16. Navas S, Seo SH, Chua MM, Sarma JD, Lavi E, Hingley ST, Weiss SR (2001). Murine coronavirus spike protein determines the ability of the virus to replicate in the liver and cause hepatitis. J Virol 75: 2452-2457.
17. Ontiveros E, Kuo L, Masters PS, Perlman S (2001). Inactivation of expression of gene 4 of mouse hepatitis virus strain JHM does not affect virulence in the murine CNS. Virology 289: 230-238.
18. Phillips JJ, Chua MM, Lavi E, Weiss SR (1999). Pathogenesis of chimeric MHV4/MHV-A59 recombinant viruses: The murine coronavirus spike protein is a major determinant of neurovirulence. J Virol 73: 7752-7760.
19. Phillips JJ, Chua M, Seo SH, Weiss SR (2001). Multiple regions of the murine coronavirus spike glycoprotein influence neurovirulence. J NeuroVirol 7: 421-431.
20. Sanger F, Nicklen S, Coulson AP (1977). DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463-5467.
21. Schwarz B, Routledge E, Siddell SG (1990). Murine coronavirus nonstructural protein ns2 is not essential for virus replication in transformed cells. J Virol 64: 4784-4791.
22. Slifka MK, Pagarigan R, Mena I, Feuer R, Whitton JL (2001). Using recombinant coxsackievirus B3 to evaluate the induction and protective efficacy of CD8+ T cells during picornavirus infection. J Virol 75: 2377-2387.
23. Smith BN, Banfield BW, Smeraski CA, Wilcox CL, Dudek FE, Enquist LW, Pickard GE (2000). Pseudorabies virus expressing enhanced green fluorescent protein: a tool for in vitro electrophysiological analysis of transsynaptically labeled neurons in identified central nervous system circuits. Proc Natl Acad Sci USA 97: 9264-9269.
24. Sutherland RM, Chua MM, Lavi E, Weiss SR, Paterson Y (1997). CD4+ and CD8+ T cells are not major effectors of mouse hepatitis virus A59-induced demyelinating disease. J NeuroVirol 3: 225-228.
25. Yokomori K, Lai MMC (1991). Mouse hepatitis virus S sequence reveals that nonstructural proteins ns4 and ns5a are not essential for murine coronavirus replication. J Virol 65: 5605-5608.