Multiple regions of the murine coronavirus spike glycoprotein influence neurovirulence

Journal of NeuroVirology

Volumn 7, Issue 5, 2001, Pages 421-431

  Phillips, J J ^a   Chua, M M ^a   Seo, S H ^a   Weiss, S R ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

Encephalitis; Mouse hepatitis virus; Neurovirulence; Recombinant coronaviruses

Indexed keywords

PROTEIN SUBUNIT; VIRUS GLYCOPROTEIN;

ANIMAL CELL; ARTICLE; BRAIN; CENTRAL NERVOUS SYSTEM INFECTION; CHIMERA; CONTROLLED STUDY; GENETIC RECOMBINATION; IN VITRO STUDY; IN VIVO STUDY; MURINE HEPATITIS CORONAVIRUS; NONHUMAN; PATHOGENESIS; PHENOTYPE; PRIORITY JOURNAL; VIRUS ATTENUATION; VIRUS GENE; VIRUS LOAD; VIRUS RECOMBINANT; VIRUS REPLICATION; VIRUS VIRULENCE;

ANIMALS; CATS; CELL LINE; LETHAL DOSE 50; MALE; MEMBRANE FUSION; MEMBRANE GLYCOPROTEINS; MICE; MICE, INBRED C57BL; MURINE HEPATITIS VIRUS; PLAQUE ASSAY; PROTEIN SUBUNITS; RECOMBINANT FUSION PROTEINS; RECOMBINATION, GENETIC; RNA, VIRAL; SPECIFIC PATHOGEN-FREE ORGANISMS; VIRAL ENVELOPE PROTEINS; VIRULENCE; VIRUS REPLICATION;

ANIMALIA; CORONAVIRUS; MURINAE; MURINE HEPATITIS VIRUS; MURINE HEPATITIS VIRUS STRAIN 4;

EID: 0034767475     PISSN: 13550284     EISSN: None     Source Type: Journal    
DOI: 10.1080/135502801753170273     Document Type: Article

References (47)

1. A clustering of RNA recombination sites adjacent to a hypervariable region of the peplomer gene of murine coronavirus
2. Redefined nomenclature for members of the carcinoembryonic antigen family
3. b-restricted cytotoxic T cell response
4. Murine hepatitis virus-4 (strain JHM)-induced neurologic disease is modulated in vivo by monoclonal antibody
5. + T-cell epitopes within the surface glycoprotein of a neurotropic coronavirus and correlation with pathogenicity
6. Monoclonal antibodies to murine hepatitis virus-4 (strain JHM) define the viral glycoprotein responsible for attachment and cell-cell fusion
7. Site-specific alteration of murine hepatitis virus type 4 peplomer glycoprotein E2 results in reduced neurovirulence
8. Evidence for a coiled-coil structure in the spike proteins of coronaviruses
9. Cloning of the mouse hepatitis virus (MHV) receptor: Expression in human and hampster cell lines confers susceptibility to MHV
10. The V5A13.1 envelope glycoprotein deletion mutant of mouse hepatitis virus type-4 is neuroattenuated by its reduced rate of spread in the central nervous system
11. Analysis of a recombinant mouse hepatitis virus expressing a foreign gene reveals a novel aspect of coronavirus transcription
12. Antigenic relationships of murine coronaviruses: Analysis using monoclonal antibodies to JHM (MHV-4) virus
13. Pathogenicity of antigenic variants of murine coronavirus JHM selected with monoclonal antibodies
14. Proteolytic cleavage of the E2 glycoprotein of murine coronavirus: Host-dependent differences in proteolytic cleavage and cell fusion
15. A role for naturally occurring variation of the murine coronavirus spike protein in stabilizing association with the cellular receptor
16. Cell receptor-independent infection by a neurotropic murine coronavirus
17. Alteration of pH dependence of coronavirus-induced cell fusion: Effect of mutations in the spike glycoprotein
18. Neutralization-resistant variants of a neurotropic coronavirus are generated by deletions within the aminoterminal half of the spike glycoprotein
19. Fusion-defective mutants of mouse hepatitis virus A59 contain a mutation in the spike protein cleavage signal
20. Single amino acid changes in the S2 subunit of the MHV surface glycoprotein confer resistance to neutralization by S1 subunit-specific monoclonal antibody
21. MHV-A59 fusion mutants are attenuated and display altered hepatotropism
22. Repair and mutagenesis of the genome of a deletion mutant of the murine coronavirus mouse hepatitis virus by targeted RNA recombination
23. Localization of neutralizing epitopes and the receptor-binding site within the amino-terminal 330 amino acids of the murine coronavirus spike protein
24. Retargeting of coronavirus by substitution of the spike glycoprotein ectodomain: Crossing the host cell species barrier
25. Limbic encephalitis after inhalation of a murine coronavirus
26. The organ tropism of mouse hepatitis virus strain A59 is dependent on dose and route of inoculation
27. Targeted recombination within the spike gene of murine coronavirus mouse hepatitis virus-A59: Q159 is a determinant of hepatotropism
28. Primary structure of the glycoprotein E2 of coronavirus MHV-A59 and identification of the trypsin cleavage site
29. Molecular cloning, a laboratory manual
30. Optimization of targeted RNA recombination and mapping of a novel nucleocapsid gene mutation in the coronavirus mouse hepatitis virus
31. Sequence analysis reveals extensive polymorphism and evidence of deletions within the E2 glycoprotein gene of several strains of murine hepatitis virus
32. Cytotoxic T cell-resistant variants are selected in a virus-induced demyelinating disease
33. Pathogenesis of chimeric MHV4/MHV-A59 recombinant viruses: The murine coronavirus spike protein is a major determinant of neurovirulence
34. Assembly of Theiler's virus recombinants used in mapping determinants of neurovirulence
35. Isolation and genetic characterization of temperature sensitive mutants of simian rotavirus SA11
36. Intracellular complexes of viral spike and cellular receptor accumulate during cytopathic murine coronavirus infections
37. A simple method of estimating fifty per cent points
38. Identification of spike protein residues of murine coronavirus responsible for receptor-binding activity by use of soluble receptor-resisitant mutants
39. Methods in viral pathogenesis
40. Conformational change of the coronavirus peplomer glycoprotein at pH 8.0 and 37°C correlates with virus aggregation and virus-induced cell fusion
41. Comparison of six different murine coronavirus JHM variants by monoclonal antibodies against the E2 glycoprotein
42. A 12-amino acid stretch in the hypervariable region of the spike protein S1 subunit is critical for cell fusion activity of mouse hepatitis virus
43. Sequence analysis of the spike protein gene of murine coronavirus variants: Study of genetic sites affecting neuropathogenicity
44. The peplomer protein E2 of coronavirus JHM as a determinant of neurovirulence: Definition of critical epitopes by variant analysis
45. Monoclonal antibodies to the peplomer glycoprotein of coronavirus mouse hepatitis virus identify two subunits and detect a conformational change in the subunit released under mild alkaline conditions
46. Receptor for mouse hepatitis virus is a member of the carcinoembryonic antigen family of glycoproteins
47. Chimeric cDNA studies of Theiler's murine encephalomyelitis virus neurovirulence