Acid hydrolysis of silk fibroins and determination of the enrichment of isotopically labeled amino acids using precolumn derivatization and high-performance liquid chromatography-electrospray ionization-mass spectrometry

Analytical Biochemistry

Volumn 311, Issue 1, 2002, Pages 19-26

  Hess, Sonja ^a   Van Beek, Jacco ^b   Pannell, Lewis K ^a  

^a NATIONAL INSTITUTES OF HEALTH   (United States)

^b ETH ZURICH   (Switzerland)

Author keywords

Amino acid; Electrospray mass spectrometry; Hydrolysis; Isotope natural products; Liquid chromatography; Marfey's reagent

Indexed keywords

AMINO ACIDS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HIGH PRESSURE LIQUID CHROMATOGRAPHY; HYDROLYSIS; ISOTOPES; LIQUID CHROMATOGRAPHY; LIQUIDS; MASS SPECTROMETRY; REAGENTS;

ELECTROSPRAY MASS SPECTROMETRY; NATURAL PRODUCTS; ON-LINE ELECTROSPRAY MASS SPECTROMETRY; PRECOLUMN DERIVATIZATIONS; SOLID-STATE NUCLEAR MAGNETIC RESONANCE; STABLE ISOTOPE TRACERS; STRUCTURAL INVESTIGATION; STRUCTURE , PROPERTIES;

ELECTROSPRAY IONIZATION;

ALANINE; AMINO ACID; CARBON 13; FIBROIN; GLYCINE; NATURAL PRODUCT; NITROGEN 15; REAGENT; SILK FIBROIN; ACETIC ACID; CARBON; DRUG DERIVATIVE; INSECT PROTEIN; MARFEY'S REAGENT; NITROBENZENE DERIVATIVE; NITROGEN;

AMINO ACID ANALYSIS; ARTICLE; DERIVATIZATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROLYSIS; ISOTOPE LABELING; LARVA; MASS SPECTROMETRY; MOTH; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; SILK; SPIDER; ANIMAL; BIOSYNTHESIS; CALIBRATION; CHEMISTRY; COMPUTER PROGRAM; DIET; ELECTROSPRAY MASS SPECTROMETRY; EXOCRINE GLAND; METABOLISM; METHODOLOGY; SILKWORM;

ARANEAE; LEPIDOPTERA;

ACETIC ACID; ALANINE; AMINO ACIDS; ANIMALS; BOMBYX; CALIBRATION; CARBON RADIOISOTOPES; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DIET; DINITROBENZENES; EXOCRINE GLANDS; FIBROINS; HYDROLYSIS; INSECT PROTEINS; LARVA; NITROGEN RADIOISOTOPES; SILK; SOFTWARE; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; SPIDERS;

EID: 1842837990     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(02)00402-5     Document Type: Article

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