Molecular cloning, nucleotide sequencing and characterization of the flagellin gene from isolates of urease-positive thermophilic Campylobacter

Research in Microbiology

Volumn 155, Issue 3, 2004, Pages 185-191

  Sekizuka, Tsuyoshi ^a   Gondo, Takayoshi ^a   Murayama, Ohoshi ^a   Kato, Yukio ^a   Moore, John E ^b   Millar, B Cherie ^b   Matsuda, Motoo ^a  

^a AZABU UNIVERSITY   (Japan)

^b BELFAST CITY HOSPITAL   (United Kingdom)

Author keywords

Cloning and sequencing; flaA; N terminal amino acid sequencing; Purified flagellin; Urease positive thermophilic Campylobacter

Indexed keywords

FLAGELLIN; GENE PRODUCT; UREASE;

AMINO ACID SEQUENCE; AMPLICON; ARTICLE; BACTERIAL GENE; BASE PAIRING; CAMPYLOBACTER COLI; CAMPYLOBACTER JEJUNI; GENE AMPLIFICATION; GENE ISOLATION; GENE LOCUS; GENETIC HETEROGENEITY; GENETIC VARIABILITY; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; THERMOPHILIC BACTERIUM;

AMINO ACID SEQUENCE; BASE SEQUENCE; CAMPYLOBACTER; CLONING, MOLECULAR; DNA, BACTERIAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FLAGELLIN; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; POLYMERASE CHAIN REACTION; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; SEQUENCE ANALYSIS, PROTEIN; UREASE;

CAMPYLOBACTER; CAMPYLOBACTER COLI; CAMPYLOBACTER JEJUNI; CAMPYLOBACTER LARI; CHARADRII;

EID: 1842471062     PISSN: 09232508     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.resmic.2003.12.003     Document Type: Article

References (18)

1. Bezian M.C., Ribou G., Barberis-Giletti C., Megraud F. Isolation of a urease positive thermophilic variant of Campylobacter lari from a patient with urinary tract infection. Eur. J. Clin. Microbiol. Infect. Dis. 9:1990;895-897.
2. Bolton F.J., Holt A.V., Hutchinson D.N. Urease-positive thermophilic campylobacters. Lancet. I:1985;1217-1218.
3. Endtz H.P., Vliegenthart J.S., Vandamme P., Weverink H.W., van den Braak N.P., Verbrugh H.A., van Belkum A. Genotypic diversity of Campylobacter lari isolated from mussels and oysters in The Netherlands. Int. J. Food Microbiol. 34:1997;79-88.
4. Guerry P., Logan S.M., Thornton S., Trust T.J. Genomic organization and expression of Campylobacter flagellin genes. J. Bacteriol. 172:1990;1853-1860.
5. Harrington C.S., Thomson-Carter F.M., Carter P.E. Evidence for recombination in the flagellin locus of Campylobacter jejuni: Implications for the flagellin gene typing scheme. J. Clin. Microbiol. 35:1997;2386-2392.
6. Kaneko A., Matsuda M., Miyajima M., Moore J.E., Murphy P.G. Urease-positive thermophilic isolates of Campylobacter isolated from seagulls (Larus spp.). Lett. Appl. Microbiol. 29:1999;7-9.
7. Logan S.M., Harris L.A., Trust T.J. Isolation and characterization of Campylobacter flagellins. J. Bacteriol. 169:1987;5072-5077.
8. Logan S.M., Kelly J.F., Thibault P., Ewing C.P., Guerry P. Structural heterogeneity of carbohydrate modifications affects serospecificity of Campylobacter flagellins. Mol. Microbiol. 46:2002;587-597.
9. Logan S.M., Trust T.J. Molecular identification of surface protein antigens of Campylobacter jejuni. Infect. Immun. 42:1983;675-682.
10. Matsuda M., Kaneko A., Fukuyama M., Ito T., Shingaki M., Inoue M., Moore J.E., Murphy P.G., Ishida Y. First finding of urease-positive thermophilic isolates of Campylobacter in river water in the Far East, namely, in Japan, and their phenotypic and genotypic characterization. J. Appl. Bacteriol. 81:1996;608-612.
11. Megraud F., Chevrier D., Desplaces N., Sedallian A., Guesdon J.L. Urease-positive thermophilic Campylobacter (Campylobacter laridis variant) isolated from an appendix and from human feces. J. Clin. Microbiol. 26:1988;1050-1051.
12. Meinersmann R.J., Helsel L.O., Fields P.I., Hiett K.L. Discrimination of Campylobacter jejuni isolates by fla gene sequencing. J. Clin. Microbiol. 35:1997;2810-2814.
13. Nachamkin I., Bohachick K., Patton C.M. Flagellin gene typing of Campylobacter jejuni by restriction fragment length polymorphism analysis. J. Clin. Microbiol. 31:1993;1531-1536.
14. Nuijten P.J.M., van Asten F.J.A.M., Gaastra W., van der Zeijst B.A.M. Structural and functional analysis of two Campylobacter jejuni flagellin genes. J. Biol. Chem. 265:1990;17798-17804.
15. Owen R.J., Leaper S. Base composition, size and nucleotide-sequence similarities of genome deoxyribonucleic acids from species of the genus Campylobacter. FEMS Microbiol. Lett. 12:1981;395-400.
16. Thibault P., Logan S.M., Kelly J.F., Brisson J.R., Ewing C.P., Trust T.J., Guerry P. Identification of the carbohydrate moieties and glycosylation motifs in Campylobacter jejuni. flagellin, J. Biol. Chem. 276:2001;34862-34870.
17. Walker R.I., Caldwell M.B., Lee E.C., Guerry P., Trust T.J., Ruiz-Palacios G.M. Pathophysiology of Campylobacter enteritis. Microbiol. Rev. 50:1986;81-94.
18. Wilson I.G., Moore J.E. Presence of Salmonella spp. and Campylobacter spp. in shellfish. Epidemiol. Infect. 116:1996;147-153.