RanGAP1*SUMO1 is phosphorylated at the onset of mitosis and remains associated with RanBP2 upon NPC disassembly

Journal of Cell Biology

Volumn 164, Issue 7, 2004, Pages 965-971

  Swaminathan, Sowmya ^a,b   Kiendl, Florian ^a   Körner, Roman ^a   Lupetti, Raffaella ^a   Hengst, Ludger ^a   Melchior, Frauke ^a  

^a MAX PLANCK INSTITUTE OF BIOCHEMISTRY   (Germany)

^b Nature Cell Biology   (United Kingdom)

Author keywords

Cyclin B Cdk1; RanBP2 Nup358; RanGAP1; SUMO; Ubc9

Indexed keywords

CYCLIN B; CYCLIN DEPENDENT KINASE 1; ENZYME; LIGASE; NOCODAZOLE; NUCLEOPORIN; PROTEIN UBC9; RAN ACTIVATING PROTEIN; RAN PROTEIN; RANBP2 PROTEIN; RANGAP1; SERINE; SUMO 1 E3 LIGASE; SUMO 1 PROTEIN; SUMO E2 CONJUGATING ENZYME; TYROSINE; UNCLASSIFIED DRUG; CHAPERONE; GUANOSINE TRIPHOSPHATASE ACTIVATING PROTEIN; RAN BINDING PROTEIN 2; RAN-BINDING PROTEIN 2; RANGAP1 PROTEIN, HUMAN;

ARTICLE; CELL NUCLEUS MEMBRANE; COMPLEX FORMATION; DISSOCIATION; HUMAN; HUMAN CELL; IN VITRO STUDY; IN VIVO STUDY; INTERPHASE; INTRACELLULAR TRANSPORT; MITOSIS; NUCLEAR PORE COMPLEX; NUCLEAR PORE COMPLEX DISASSEMBLY; PRIORITY JOURNAL; PROTEIN FUNCTION; PROTEIN MODIFICATION; PROTEIN PHOSPHORYLATION; AMINO ACID SEQUENCE; BINDING SITE; CELL CYCLE; CHEMISTRY; FLUORESCENCE MICROSCOPY; HELA CELL; METABOLISM; MOLECULAR GENETICS; NUCLEAR PORE; PHOSPHORYLATION; PHYSIOLOGY; ULTRASTRUCTURE;

VERTEBRATA;

AMINO ACID SEQUENCE; BINDING SITES; CELL CYCLE; GTPASE-ACTIVATING PROTEINS; HELA CELLS; HUMANS; INTERPHASE; MICROSCOPY, FLUORESCENCE; MITOSIS; MOLECULAR CHAPERONES; MOLECULAR SEQUENCE DATA; NUCLEAR ENVELOPE; NUCLEAR PORE; NUCLEAR PORE COMPLEX PROTEINS; PHOSPHORYLATION; SUMO-1 PROTEIN;

EID: 1842457017     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.200309126     Document Type: Article

References (27)

1. Arnaoutov, A., and M. Dasso. 2003. The Ran GTPase regulates kinetochore function. Dev. Cell. 5:99-111.
2. Bonifacino, J.S., M. Dasso, J. Lippincott-Schwartz, J.B. Harford, and K.M. Yamada. 1999. Current Protocols in Cell Biology. John Wiley and Sons, New York. Also available at http://www.mrw2.interscience.wiley.com/cponline.
3. Favreau, C., H.J. Worman, R.W. Wozniak, T. Frappier, and J.C. Courvalin. 1996. Cell cycle-dependent phosphorylation of nucleoporins and nuclear pore membrane protein Gp210. Biochemistry. 35:8035-8044.
4. Gobom, J., E. Nordhoff, E. Mirgorodskaya, R. Ekman, and P. Roepstorff. 1999. Sample purification and preparation technique based on nano-scale reversed-phase columns for the sensitive analysis of complex peptide mixtures by matrix-assisted laser desorption/ionization mass spectrometry. J. Mass Spectrom. 34:105-116.
5. Guarguaglini, G., L. Renzi, F. D'Ottavio, B. Di Fiore, M. Casenghi, E. Cundari, and P. Lavia. 2000. Regulated Ran-binding protein 1 activity is required for organization and function of the mitotic spindle in mammalian cells in vivo. Cell Growth Differ. 11:455-465.
6. Hengst, L., and S.I. Reed. 1996. Translational control of p27Kip1 accumulation during the cell cycle. Science. 271:1861-1864.
7. Hengst, L., and S.I. Reed. 1998. Inhibitors of the Cip/Kip family. Curr. Top. Microbiol. Immunol. 227:25-41.
8. Hengst, L., U. Gopfert, H.A. Lashuel, and S.I. Reed. 1998. Complete inhibition of Cdk/cyclin by one molecule of p21 (Cip1). Genes Dev. 12:3882-3888.
9. Joseph, J., S.H. Tan, T.S. Karpova, J.G. McNally, and M. Dasso. 2002. SUMO-1 targets RanGAP1 to kinetochores and mitotic spindles. J. Cell Biol. 156:595-602.
10. Kapoor, T.M., T.U. Mayer, M.L. Coughlin, and T.J. Mitchison. 2000. Probing spindle assembly mechanisms with monastrol, a small molecule inhibitor of the mitotic kinesin, Eg5. J. Cell Biol. 150:975-988.
11. Kreegipuu, A., N. Blom, and S. Brunak. 1999. PhosphoBase, a database of phosphorylation sites: release 2.0. Nucleic Acids Res. 27:237-239.
12. Li, H.Y., D. Wirtz, and Y. Zheng. 2003. A mechanism of coupling RCC1 mobility to RanGTP production on the chromatin in vivo. J. Cell Biol. 160:635-644.
13. Macaulay, C., E. Meier, and D.J. Forbes. 1995. Differential mitotic phosphorylation of proteins of the nuclear pore complex. J. Biol. Chem. 270:254-262.
14. Mahajan, R., C. Delphin, T. Guan, L. Gerace, and F. Melchior. 1997. A small ubiquitin-related polypeptide involved in targeting RanGAP1 to nuclear pore complex protein RanBP2. Cell. 88:97-107.
15. Matunis, M.J., E. Coutavas, and G. Blobel. 1996. A novel ubiquitin-like modification modulates the partitioning of the Ran-GTPase-activating protein RanGAP1 between the cytosol and the nuclear pore complex. J. Cell Biol. 135:1457-1470.
16. Matunis, M.J., J. Wu, and G. Blobel. 1998. SUMO-1 modification and its role in targeting the Ran GTPase-activating protein, RanGAP1, to the nuclear pore complex. J. Cell Biol. 140:499-509.
17. Melchior, F. 1998. Nuclear protein import in a permeabilized cell assay. Methods Mol. Biol. 88:265-273.
18. Pichler, A., A. Gast, J.S. Seeler, A. Dejean, and F. Melchior. 2002. The nucleoporin RanBP2 has SUMO1 E3 ligase activity. Cell. 108:109-120.
19. Quimby, B.B., and M. Dasso. 2003. The small GTPase Ran: interpreting the signs. Curr. Opin. Cell Biol. 15:338-344.
20. Salina, D., P. Enarson, J.B. Rattner, and B. Burke. 2003. Nup358 integrates nuclear envelope breakdown with kinetochore assembly. J. Cell Biol. 162:991-1001.
21. Seeler, J.S., and A. Dejean. 2003. Nuclear and unclear functions of SUMO. Nat. Rev. Mol. Cell Biol. 4:690-699.
22. Shevchenko, A., M. Wilm, O. Vorm, and M. Mann. 1996. Mass spectrometric sequencing of proteins from silver-stained polyacrylamide gels. Anal. Chem. 68:850-858.
23. Takizawa, C.G., and D.O. Morgan. 2000. Control of mitosis by changes in the subcellular location of cyclin-B1-Cdk1 and Cdc25C. Curr. Opin. Cell Biol. 12:658-665.
24. Toyoshima-Morimoto, F., E. Taniguchi, N. Shinya, A. Iwamatsu, and E. Nishida. 2001. Polo-like kinase 1 phosphorylates cyclin B1 and targets it to the nucleus during prophase. Nature. 410:215-220.
25. Walther, T.C., P. Askjaer, M. Gentzel, A. Habermann, G. Griffiths, M. Wilm, I.W. Mattaj, and M. Hetzer. 2003. RanGTP mediates nuclear pore complex assembly. Nature. 424:689-694.
26. Yang, J., and S. Kornbluth. 1999. All aboard the cyclin train: subcellular trafficking of cyclins and their CDK partners. Trends Cell Biol. 9:207-210.
27. Zhang, H., H. Saitoh, and M.J. Matunis. 2002. Enzymes of the SUMO modification pathway localize to filaments of the nuclear pore complex. Mol. Cell. Biol. 22:6498-6508.