A mechanism of coupling RCC1 mobility to RanGTP production on the chromatin in vivo

Journal of Cell Biology

Volumn 160, Issue 5, 2003, Pages 635-644

  Li, Hoi Yeung ^a   Wirtz, Denis ^b   Zheng, Yixian ^a  

^a GEOPHYSICAL LABORATORY   (United States)

^b Departments of Orthopaedic Surgery   (United States)

Author keywords

Chromatin; Fluorescence intensity; Nuclear; Nucleotide exchange; Ran

Indexed keywords

GREEN FLUORESCENT PROTEIN; GUANINE NUCLEOTIDE; GUANINE NUCLEOTIDE EXCHANGE FACTOR; PROTEIN RCC1; RAN PROTEIN; REGULATOR OF CHROMOSOME CONDENSATION; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BLEACHING; CELL NUCLEUS MEMBRANE; CELL STRAIN 3T3; CELL STRAIN BHK; CHO CELL; CHROMATIN; CHROMOSOME CONDENSATION; COMPLEX FORMATION; CONTROLLED STUDY; DISSOCIATION; ENZYME ACTIVITY; FLUORESCENCE MICROSCOPY; IN VIVO STUDY; INTERPHASE; MITOSIS; MOUSE; NONHUMAN; NUCLEOCYTOPLASMIC TRANSPORT; PRIORITY JOURNAL; XENOPUS;

3T3 CELLS; ANIMALS; CELL COMPARTMENTATION; CELL CYCLE; CELL CYCLE PROTEINS; CELL NUCLEUS; CHROMATIN; DNA-BINDING PROTEINS; EUKARYOTIC CELLS; FEMALE; GUANINE NUCLEOTIDE EXCHANGE FACTORS; HUMANS; MALE; MICE; NUCLEAR PROTEINS; OOCYTES; PROTEIN TRANSPORT; RAN GTP-BINDING PROTEIN; SPERMATOZOA; TEMPERATURE; XENOPUS LAEVIS; XENOPUS PROTEINS;

ANIMALIA;

EID: 0037416202     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.200211004     Document Type: Article

References (23)

1. Bilbao-Cortes, D., M. Hetzer, G. Langst, P.B. Becker, and I.W. Mattaj. 2002. Ran binds to chromatin by two distinct mechanisms. Curr. Biol. 12:1151-1156.
2. Bischoff, F.R., and H. Ponstingl. 1991. Catalysis of guanine nucleotide exchange on Ran by the mitotic regulator RCC1. Nature. 354:80-82.
3. Bischoff, F.R., and H. Ponstingl. 1995. Catalysis of guanine nucleotide exchange of Ran by RCC1 and stimulation of hydrolysis of Ran-bound GTP by Ran-GAP1. Methods Enzymol. 257:135-144.
4. Dasso, M. 2002. The Ran GTPase: theme and variations. Curr. Biol. 12:R502-R508.
5. Dasso, M., S.T. Azuma, T. Ohba, and T. Nishimoto. 1994. A mutant form of the Ran/TC4 protein disrupts nuclear function in Xenopus laevis egg extracts by inhibiting the RCC1 protein, a regulator of chromosome condensation. EMBO J. 13:5732-5744.
6. Kalab, P., K. Weis, and R. Heald. 2002. Visualization of a Ran-GTP gradient in interphase and mitotic Xenopus egg extracts. Science. 295:2452-2456.
7. Kimura, H., and P.R. Cook. 2001. Kinetics of core histones in living cells: little exchange of H3 and H4 and some rapid exchange of H2B. J. Cell Biol. 153:1341-1354.
8. Klebe, C., F.R. Bischoff, H. Ponstingl, and A. Wittinghofer. 1995a. Interaction of the nuclear GTP-binding protein Ran with its regulatory proteins RCC1 and RanGAP1. Biochemistry. 34:639-647.
9. Klebe, C., H. Prinz, A. Wittinghofer, and R.S. Goody. 1995b. The kinetic mechanism of Ran-nucleotide exchange catalyzed by RCC1. Biochemistry. 34:12543-12552.
10. Kornbluth, S., M. Dasso, and J. Newport. 1994. Evidence for a dual role for TC4 protein in regulating nuclear structure and cell cycle progression. J. Cell Biol. 125:705-719.
11. Lounsbury, K.M., S.A. Richards, K.L. Carey, and I.G. Macara. 1996. Mutations with the Ran/TC4 GTPase. J. Biol. Chem. 271:32834-32841.
12. Mattaj, I., and L. Englmeier. 1998. Nucleocytoplasmic transport: the soluble phase. Annu. Rev. Biochem. 67:265-306.
13. Misteli, T., A. Gunjan, R. Hock, B. Michael, and D. Brown. 2000. Dynamic binding of histone H1 to chromatin in living cells. Nature. 408:877-881.
14. Murray, A.W., and M.W. Kirschner. 1989. Cyclin synthesis drives the early embryonic cell cycle. Nature. 339:275-280.
15. Nemergut, M., C.A. Mizzen, T. Stukenberg, C.D. Allis, and I.G. Macara. 2001. Chromatin docking and exchange activity enhancement of RCC1 by histones H2A and H2B. Science. 292:1540-1543.
16. Nishitani, H., M. Ohtsubo, K. Yamashita, H. Iida, J. Pines, H. Yasudo, Y. Shibata, T. Hunter, and T. Nishimoto. 1991. Loss of RCC1, a nuclear DNA-binding protein, uncouples the completion of DNA replication from the activation of cdc2 protein kinase and mitosis. EMBO J. 10:1555-1564.
17. Ohtsubo, M., H. Okazaki, and T. Nishimoto. 1989. The RCC1 protein, a regulator for the onset of chromosome condensation locates in the nucleus and binds to DNA. J. Cell Biol. 109:1389-1397.
18. Phair, R., and T. Misteli. 2000. High mobility of proteins in the mammalian cell nucleus. Nature. 404:604-609.
19. Renault, L., J. Kuhlmann, A. Henkel, and A. Wittinghofer. 2001. Structural basis for guanine nucleotide exchange on Ran by the regulator of chromosome condensation (RCC1). Cell. 105:245-255.
20. Seino, H., S. Hisamoro, T. Uzawa, T. Sekiguchi, and T. Nishimoto. 1992. DNA-binding domain of RCC1 protein is not essential for coupling mitosis with DNA replication. J. Cell Sci. 102:393-400.
21. Seki, T., N. Hayashi, and T. Nishimoro. 1996. RCC1 in the Ran pathway. J. Biochem (Tokyo). 120:207-214.
22. Wilde, A., and Y. Zheng. 1999. Stimulation of microtubule aster formation and spindle assembly by the small GTPase Ran. Science. 284:1359-1362 (see comment).
23. Zhang, L., T.J. Kearing, A. Wilde, G.G. Borisy, and Y. Zheng. 2000. The role of Xgrip210 in gamma tubulin ring complex assembly and centrosome recruitment. J. Cell Biol. 151:1525-1535.