Rapid typing and elucidation of new secondary metabolites of intact cyanobacteria using MALDI-TOF mass spectrometry

Nature Biotechnology

Volumn 15, Issue 9, 1997, Pages 906-909

  Erhard, Marcel ^a   von Döhren, Hans ^a   Jungblut, Peter ^b  

^a TECHNISCHE UNIVERSITÄT BERLIN   (Germany)

^b MAX PLANCK INSTITUTE FOR INFECTION BIOLOGY   (Germany)

Author keywords

Anabaenopeptolins; Cyanobacteria; MALDI TOF; Microcystin; PSD; Secondary metabolites

Indexed keywords

ARTICLE; BACTERIAL METABOLISM; CHEMICAL ANALYSIS; CYANOBACTERIUM; MASS SPECTROMETRY; NONHUMAN; PEPTIDE ANALYSIS; PRIORITY JOURNAL; TOXIN ANALYSIS;

ANABAENA; BACTERIAL PROTEINS; CYANOBACTERIA; MICROCYSTINS; PEPTIDES, CYCLIC; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

ALGAE; BACTERIA (MICROORGANISMS); CYANOBACTERIA;

EID: 1842326197     PISSN: 10870156     EISSN: 15461696     Source Type: Journal    
DOI: 10.1038/nbt0997-906     Document Type: Article

References (19)

1. Watanabe, M.F., Harada, K., Carmichael, W.W., and Fujiki, H. (ads.) 1996. Toxic microcystis, CRC Press, Boca Raton, Florida.
2. MacKintosh, R.W., Dalby, K.N., Campebell, D.G., Cohen, P.T.W., Cohen, P., MacKintosh, C. 1995. The cyanobacterial toxin microcystin binds covalently to cysteine-273 on protein phosphatase 1. FEBS Lett. 371:236-240.
3. Martin, C, Oberer, L., Ino, T., König, W.A., Busch, M., and Weckesser, J. 1993. Cyanopeptolins, new depsipeptides from the cyanobacterium Microcystis sp. PCC 7806. J. Antibiot. 46:1550-1556.
4. Ischida, K., Murakami, M., Matsuda, H., and Yamaguchi, K. 1995. Micropeptin 90, a plasmin and trypsin inhibitor from the blue-green alga Microcystis aeruginosa NIES 90. Tetrahedron Lett. 36:3535-3538.
5. Harada, K., Mayumi, T., Shimada, T., Suzuki, M., Kondo, F., and Watanabe, M.F. 1993. Occurrence of four depsipeptides, aeruginopeptins, together with microcystins from toxic cyanobacteria. Tetrahedron Lett. 34:6091-6094.
6. Harada, K., Fujiki, K., Shimada, T., Suzuki, M., Sano, H., Adachi, K., et al. 1995. Two cyclic peptides, anabaenopeptins, a third group of bioactive compounds from the cyanobacterium Anabaena flos.aquae NRC 525-17. Tetrahedron Lett. 36:1511-1514.
7. Kleinkauf, H. and von Döhren, H. 1997. Peptide antibiotics, pp. 277-322 in Products of secondary metabolism. Kleinkauf, H. and von Döhren, H. (eds.) Vol. 7 of Biotechnology. Rehm, H., Reed, LG., Pühler, Α., Stadler, P. (eds.), 2nd ed. Verlag Chemie Weinheim.
8. Claydon, M.A., Davey, S.N., Edwards-Jones, V., and Gordon, D.B. 1996. The rapid identification of intact microorganisms using mass spectrometry. Nature Biotechnology 14:1584-1586.
9. Krishnamurthy, T., Ross, P.L., and Rajamani, U. 1996. Detection of pathogenic and non-pathogenic bacteria by matrix-assisted laser desorption/ionization time of flight mass spectrometry. Rapid Commun. Mass Spectrom. 10:883-888.
10. Liang, X., Zheng, K., Qian, M.G., and Lubman, D. M. 1996. Determination of bacterial protein profiles by matrix-assisted laser desorption/ionization time of flight mass spectrometry with high-performance liquid chromatography. Rapid Commun. Mass Spectrom. 10:1219-1226.
11. Holland, R.D., Wilkes, J.G., Rafii, F., Sutherland, J.B., Persons, C.C., Voorhees, K.J., et al. 1996. Rapid identification of intact whole bacteria based on spectral patterns using matrix-assisted laser desorption/ionization time of-fligh mass spectrometry. Rapid Commun. Mass Spectrom. 10:1227-1232.
12. Meiβner, K., Dittmann, E., and Börner, T. 1996. Toxic and non-toxic strains of cyanobacterium Microcystis aeruginosa contain sequences homologous to peptide synthetase genes. FEMS Microbiol. Lett. 135:295-303.
13. Sivonen, K., Namikoshi, M., Evans, W.R., Carmichael, W.W., Sun, F., Rouhiainen, L., et al. 1992. Isolation and characterization of a variety of microcystins from seven strains of the cyanobacterial genus Anabaena. Appl. Environ. Microbiol. 58:2495-2500.
14. Watanabe, M.M. and Nozaki, H. 1994. NIES-Collection; List of strains; fourth edition, Microalgae and protozoa. Microbial Culture Collection, National Institute for Environmental Studies, Environment Agency, Japan.
15. Shi, L., Carmichael, W.W., and Miller, I. 1995. Immuno-gold localization of heptotoxins in cyanobacterial cells. Arch. Microbiol. 163:7-15.
16. Klose, J. and Kobalz, U. 1995. Two-dimensional electrophoresis of proteins: An updated protocol and implications for a functional analysis of the genome. Electrophoresis 16:1034-1059.
17. Jungblut, P., Thiede, B., Zimny-Arndt, U., Müller, E.-C., Scheler, C, Wittmann-Liebold, B., et al. 1996. Resolution power of two-dimensional electrophoresis and identification of proteins from gels. Electrophoresis 17: 839-847.
18. Sano, T. and Kaya, K. 1995. Oscillamide Y, a chymotrypsin inhibitor from toxic Oscillatoria agardhii. Tetrahedron Lett. 36:5933-5936.
19. Williams, D.E., Craig, M., Holmes, C.F.B., and Andersen, R.J. 1996. Ferintoic acid A and B, new cyclic hexapeptides from freshwater cyanobacterium Microcystis aeruginosa. J. Natl. Prod. 59:570-575.