The use of small interfering RNA to elucidate the activity and function of ion channel genes in an intact tissue

Journal of Pharmacological and Toxicological Methods

Volumn 51, Issue 3 SPEC. ISS., 2005, Pages 253-262

  Gurney, Alison M ^a   Hunter, Elaine ^a  

^a UNIVERSITY OF STRATHCLYDE   (United Kingdom)

Author keywords

Artery; Gene silencing; K channels; Methods; Organ culture; RNA interference; siRNA; Smooth muscle; TASK 1; Transfection

Indexed keywords

ION CHANNEL; POTASSIUM CHANNEL; PROTEIN; SMALL INTERFERING RNA; TASK 1 PROTEIN; UNCLASSIFIED DRUG;

ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; GENE EXPRESSION; GENE FUNCTION; GENE SILENCING; GENETIC TRANSFECTION; IMMUNOHISTOCHEMISTRY; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; RNA SEQUENCE;

MAMMALIA;

EID: 18144428365     PISSN: 10568719     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vascn.2004.08.012     Document Type: Article

References (42)

1. A. Abdelgany, M. Wood, and D. Beeson Allele-specific silencing of a pathogenic mutant acetylcholine receptor subunit by RNA interference Human Molecular Genetics 12 2003 2637 2644
2. B.L. Bass The short answer Nature 411 2001 428 429
3. E. Bernstein, A.A. Caudy, S.M. Hammond, and G.J. Hannon Role for a bidentate ribonuclease in the initiation step of RNA interference Nature 409 2001 363 366
4. T.R. Brummelkamp, R. Bernards, and R. Agami A system for stable expression of short interfering RNAs in mammalian cells Science 296 2002 550 553
5. A.M. Chalk, C. Wahlestedt, and E.L. Sonnhammer Improved and automated prediction of effective siRNA Biochemical and Biophysical Research Communications 319 2004 264 274
6. 2+-permeable channel linked to the regulation of cell volume in liver cells obtained using small interfering RNA targeted against TRPC1 Biochemical Journal 373 2003 327 336
7. H. Ding, D.S. Schwarz, A. Keene, E.B. Affar, L. Fenton, and X. Xia Selective silencing by RNAi of a dominant allele that causes amyotrophic lateral sclerosis Aging Cell 2 2003 209 217
8. G. Dorn, S. Patel, G. Wotherspoon, M. Hemmings-Mieszczak, J. Barclay, and F.J. Natt siRNA relieves chronic neuropathic pain Nucleic Acids Research 32 2004 e49
9. J. Downward Science, medicine, and the future, RNA interference BMJ 328 2004 1245 1248
10. D.M. Dykxhoorn, C.D. Novina, and P.A. Sharp Killing the messenger: Short RNAs that silence gene expression Nature Reviews. Molecular Cell Biology 4 2003 457 467
11. S.M. Elbashir, J. Harborth, W. Lendeckel, A. Yalcin, K. Weber, and T. Tuschl Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells Nature 411 2001 494
12. S.M. Elbashir, J. Harborth, K. Weber, and T. Tuschl Analysis of gene function in somatic mammalian cells using small interfering RNAs Methods 26 2002 199
13. S.M. Elbashir, J. Martinez, A. Patkaniowska, W. Lendeckel, and T. Tuschl Functional anatomy of siRNA for mediating efficient RNAi in Drosophila melanogaster embryo lysate EMBO Journal 20 2001 6877 6888
14. A. Fire, S. Xu, M.K. Montgomery, S.A. Kostas, S.E. Driver, and C.C. Mello Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans Nature 391 1998 806 811
15. B. Gaudilliere, Y. Shi, and A. Bonni RNA interference reveals a requirement for myocyte enhancer factor 2A in activity-dependent neuronal survival The Journal of Biological Chemistry 277 2002 46442 46446
16. A.M. Gurney, O.N. Osipenko, D. MacMillan, K.M. McFarlane, R.J. Tate, and F.E. Kempsill Two-pore domain K channel, TASK-1, in pulmonary artery smooth muscle cells Circulation Research 93 2003 957 964
17. L. Hedley, S.B. Phagoo, and I.F. James Measurement of intracellular calcium in cell populations loaded with aequorin: Neurokinin-1 responses in U373MG cells Analytical Biochemistry 236 1996 270 274
18. M. Hemmings-Mieszczak, G. Dorn, F.J. Natt, J. Hall, and W.L. Wishart Independent combinatorial effect of antisense oligonucleotides and RNAi-mediated specific inhibition of the recombinant rat P2X3 receptor Nucleic Acids Research 31 2003 2117 2126
19. T. Holen, and C.V. Mobbs Lobotomy of genes: Use of RNA interference in neuroscience Neuroscience 126 2004 1 7
20. J.D. Hommel, R.M. Sears, D. Georgescu, D.L. Simmons, and R.J. DiLeone Local gene knockdown in the brain using viral-mediated RNA interference Nature Medicine 9 2003 1539 1544
21. G. Hutvagner, J. McLachlan, A.E. Pasquinelli, E. Balint, T. Tuschl, and P.D. Zamore A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNA Science 293 2001 834 838
22. G. Hutvagner, and P.D. Zamore A microRNA in a multiple-turnover RNAi enzyme complex Science 297 2002 2056 2060
23. S. Kloss, H. Furneaux, and A. Mulsch Post-transcriptional regulation of soluble guanylyl cyclase expression in rat aorta The Journal of Biological Chemistry 278 2003 2377 2383
24. A.M. Krichevsky, and K.S. Kosik RNAi functions in cultured mammalian neurons Proceedings of the National Academy of Sciences of the United States of America 99 2002 11926 11929
25. R.E. Lesh, A.P. Somlyo, G.K. Owens, and A.V. Somlyo Reversible permeabilization: A novel technique for the intracellular introduction of antisense oligodeoxynucleotides into intact smooth muscle Circulation Research 77 1995 220 230
26. H. Makimura, T.M. Mizuno, J.W. Mastaitis, R. Agami, and C.V. Mobbs Reducing hypothalamic AGRP by RNA interference increases metabolic rate and decreases body weight without influencing food intake BMC Neuroscience 3 2002 18
27. T. Matsuda, and C.L. Cepko Electroporation and RNA interference in the rodent retina in vivo and in vitro Proceedings of the National Academy of Sciences of the United States of America 101 2004 16 22
28. M.V. Matz, A.F. Fradkov, Y.A. Labas, A.P. Savitsky, A.G. Zaraisky, and M.L. Markelov Fluorescent proteins from nonbioluminescent Anthozoa species Nature Biotechnology 17 1999 969 973
29. M.T. McManus, and P.A. Sharp Gene silencing in mammals by small interfering RNAs Nature Reviews. Genetics 3 2002 737 747
30. O. Milhavet, D.S. Gary, and M.P. Mattson RNA interference in biology and medicine Pharmacology Reviews 55 2003 629 648
31. V. Mittal Improving the efficiency of RNA interference in mammals Nature Reviews. Genetics 5 2004 355 365
32. J.P. Morgan, T.T. DeFeo, and K.G. Morgan A chemical procedure for loading the calcium indicator acquorin into mammalian working myocardium Pflugers Archiv 400 1984 338 340
33. G.P. Nicchia, A. Frigeri, G.M. Liuzzi, and M. Svelto Inhibition of aquaporin-4 expression in astrocytes by RNAi determines alteration in cell morphology, growth, and water transport and induces changes in ischemia-related genes FASEB Journal 17 2003 1508 1510
34. C.D. Novina, and P.A. Sharp The RNAi revolution Nature 430 2004 161 164
35. J. Sambrook, E.F. Fritsch, and T. Maniatis Lysis by alkali method for plasmid preparation 2nd ed. Molecular cloning a laboratory manual book vol. 1 1989 Cold Spring Harbor Laboratory Press 1.38 1.39
36. O. Singer, A. Yanai, and I.M. Verma Silence of the genes Proceedings of the National Academy of Sciences of the United States of America 101 2004 5313 5314
37. M. Sioud Therapeutic siRNAs Trends in Pharmacological Sciences 25 2004 22 28
38. D.J. Spergel, U. Krüth, D.R. Shimshek, R. Sprengel, and P.H. Seeburg Using reporter genes to label selected neuronal populations in transgenic mice for gene promoter, anatomical, and physiological studies Progress in Neurobiology 63 2001 673 686
39. S.A. Stewart, D.M. Dykxhoorn, D. Palliser, H. Mizuno, E.Y. Yu, and D.S. An Lentivirus-delivered stable gene silencing by RNAi in primary cells RNA 9 2003 493
40. G. Sui, C. Soohoo, E.B. Affar, F. Gay, Y. Shi, and W.C. Forrester A DNA vector-based RNAi technology to suppress gene expression in mammalian cells Proceedings of the National Academy of Sciences of the United States of America 99 2002 5515
41. L. Timmons, and A. Fire Specific interference by ingested dsRNA Nature 395 1998 854
42. D.G. Welsh, A.D. Morielli, M.T. Nelson, and J.E. Brayden Transient receptor potential channels regulate myogenic tone of resistance arteries Circulation Research 90 2002 248 250