Highly efficient gene delivery into transfection-refractory neuronal and astroglial cells using a retrovirus-based vector

Journal of Microbiology and Biotechnology

Volumn 15, Issue 2, 2005, Pages 451-454

  Kim, Byung Oh ^a   Pyo, Suhkneung ^b  

^a Kyungpook National University   (South Korea)

^b Sungkyunkwan University   (South Korea)

Author keywords

Astroglial cells; Neuronal cell; Retrovirus based vector; Retrovirus mediated gene delivery

Indexed keywords

CALCIUM PHOSPHATE; CHLOROQUINE; GREEN FLUORESCENT PROTEIN; RETROVIRUS VECTOR; VIRUS DNA;

ACCURACY; ARTICLE; ASTROCYTE; BRAIN CELL; CELL LINE; CYTOTOXICITY; DNA TRANSFECTION; FEASIBILITY STUDY; FLOW CYTOMETRY; FLUORESCENCE MICROSCOPY; GENE CONTROL; GENE EXPRESSION REGULATION; GENE FUNCTION; HUMAN; HUMAN CELL; INCUBATION TIME; INTERMETHOD COMPARISON; METHODOLOGY; NERVE CELL; SENSITIVITY ANALYSIS; SIGNAL TRANSDUCTION; VIRAL GENE DELIVERY SYSTEM;

MAMMALIA; PHOENIX; UNIDENTIFIED RETROVIRUS;

EID: 18144404695     PISSN: 10177825     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (17)

1. Abul-Hassan, K., R. Walmsley, and M. Boulton. 2000. Optimization of non-viral gene transfer to human primary retinal pigment epithelial cells. Curr Eye Res. 20: 361-366.
2. Baek, C. H. and K. S. Kim. 2003. lacZ- and aph-Based Reporter vectors for in vivo expression technology. J. Microbiol. Biotechnol. 13: 872-880.
3. Baum, C., J. Dullmann, Z. Li, B. Fehse, J. Meyer. D. A. Williams, and C. von Kalle. 2003. Side effects of retroviral gene transfer into hematopoietic stem cells. Blood 15, 101: 2099-2114.
4. Colosimo, A., K. K. Gonez, A. R. Holmes, K. Kunzelmann, G. Novelli, R. W. Malone, M. J. Bennett, and D. C. Gruenert. 2000. Transfer and expression of foreign genes in mammalian cells. Biotechniques 29: 314-324.
5. Kaiser, S. and M. Toborek. 2001. Liposome-mccliated high-efficiency transfection of human endothelial cells. J. Vasc. Res. 38: 133-143.
6. Kim, H. S. and T. J. Crow. 2002. Identification and phylogeny of the human endogenous retrovirus HERV-W LTR family in human brain cDNA library and Xq21.3 region. J. Microbiol. Biotechnol. 12: 508-513.
7. Kofler, P., B. Wiesenhofer, C. Rehrl, G. Baier, G. Stockhammer, and C. Humpel. 1998. Liposome-mediated gene transfer into established CNS cell lines. primary glial cells, and in vivo. Cell transplant. 7: 175-185.
8. Onishi, M., S. Kinoshita, Y. Morikawa, A. Shibuya, J. Phillips, L. L. Lanier, D. M. Gorman, G. P. Nolan, A. Miyajima, and T. Kitamura. 1996. Applications of retrovirus-mediated expression cloning. Exp. Hematol. 24: 324-329.
9. Prasolov, V. S. 1989. Retroviral vectors--an effective system for transfer and expression of foreign genes in mammalian cells. Mol. Biol. 23: 348-355.
10. Smith, E. S., A. Mandokhot, E. E. Evans, L. Mueller, M. A. Borrello, D. M. Sahasrabudhe, and M. Zauderer. 2001. Lethality-based selection of recombinant genes in mammalian cells: Application to identifying tumor antigens. Nat. Med. 7: 967-972.
11. Song, J. S., S. B. Kim, Y. H. Lee. K. W. Lee, H. H. Jung, M. H. Kim, K. T. Kim, R. Brown, and Y. T. Kim. 2002. Adenovirus-mediated antisense expression of telomerase template RNA induces apoptosis in lung cancer cells. J. Microbiol. Biotechnol. 12: 89-95.
12. Tomi, M., H. Abukawa, Y. Nagai, T. Hata, H. Takanaga, S. Ohtsuki, T. Terasaki, and K. Hosoya. 2004. Retinal selectivity of gene expression in rat retinal versus brain capillary endothelial cell lines by differential display analysis. Mol. Vis. 10: 537-543.
13. Watanabe, S. Y., A. M. Albsoul-Younes, T. Kawano, H. Itoh, Y. Kaziro, S. Nakajima, and Y. Nakajima. 1999. Calcium phosphate-mediated transfection of primary cultured brain neurons using GFP expression its a marker: Application for single neuron electrophysiology. Neurosci. Res. 33: 71-78.
14. Wellmann, H., B. Kaltschmidt, and C. Kaltschmidt. 1999. Optimized protocol for biolistic transfection of brain slices and dissociated cultured neurons with a hand-held gene gun. J. Neurosci. Methods 92: 55-64.
15. Willis, D., B. Parameswaran, W. Shen, and G. R. Molloy. 1999. Conditions providing enhanced transfection efficiency in rat pheochromocytoma PC12 cells permit analysis of the activity of the far-upstream and proximal promoter of the brain creatine kinase gene. J. Neurosci. Methods 92: 3-13.
16. Yoon, J. H., P. H. Kim, G. T. Chun, E. Y. Choi, and S. W. Yie. 2002. Expression and receptor binding activity of fusion protein from transforming growth factor-β1 and GFP. J. Microbiol. Biotechnol. 12: 65-70.
17. Yuan, H., P. Zhai, L. M. Anderson, J. Pan, B. Thimmapaya, E. H. Koo, and N. R. Marques-Sterling. 1999. Recombinant adenovirus is an appropriate vector for endocytotic protein trafficking studies in cultured neurons. J. Neurosci. Methods 88: 45-54.