Optimized protocol for biolistic transfection of brain slices and dissociated cultured neurons with a hand-held gene gun

Journal of Neuroscience Methods

Volumn 92, Issue 1-2, 1999, Pages 55-64

  Wellmann, Henning ^a   Kaltschmidt, Barbara ^a   Kaltschmidt, Christian ^a  

^a UNIVERSITY OF FREIBURG   (Germany)

Author keywords

Biolistic gene transfer; EGFP; LacZ; Neuronal tissue; Neurons; PC12 cells

Indexed keywords

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BIOLISTIC TRANSFORMATION; CELL STRAIN; EXPRESSION VECTOR; GENE GUN; GENE TRANSFER; GENETIC TRANSFECTION; GRANULE CELL; HIPPOCAMPUS; NERVE CELL; NONHUMAN; PRIORITY JOURNAL; RAT;

ANIMALS; ANIMALS, NEWBORN; BIOLISTICS; CELLS, CULTURED; CEREBELLUM; GOLD; HIPPOCAMPUS; NEURONS; PARTICLE SIZE; PC12 CELLS; RATS; RATS, WISTAR; TRANSFECTION;

EID: 0032705581     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-0270(99)00094-1     Document Type: Article

References (24)

1. Arnold DB, Heintz N. A calcium responsive element that regulates expression of two calcium binding proteins in Purkinje cells. Proc Natl Acad Sci USA 1997;94:8842-7.
2. Arnold D, Feng L, Kim J, Heintz N. A strategy for the analysis of gene expression during neural development. Proc Natl Acad Sci USA 1994;91:9970-4.
3. Banker GA, Cowan WM. Rat hippocampal neurons in dispersed cell culture. Brain Res 1977;126:397-442.
4. Barry MA, Johnston SA. Biological features of genetic immunization. Vaccine 1997;15:788-91.
5. Biewenga JE, Destree OH, Schrama LH. Plasmid-mediated gene transfer in neurons using the biolistics technique. J Neurosci Methods 1997;71:67-75.
6. Gallo V, Ciotti MT, Coletti A, Aloisi F, Levi G. Selective release of glutamate from cerebellar granule cells differentiating in culture. Proc Natl Acad Sci USA 1982;79:7919-23.
7. Giger RJ, Ziegler U, Hermens WT, Kunz B, Kunz S, Sonderegger P. Adenovirus-mediated gene transfer in neurons: construction and characterization of a vector for heterologous expression of the axonal cell adhesion molecule axonin-1. J Neurosci Methods 1997;71:99-111.
8. Holt CE, Garlick N, Cornel E. Lipofection of cDNAs in the embryonic vertebrate central nervous system. Neuron 1990;4:203-14.
9. Jareb M, Banker G. The polarized sorting of membrane proteins expressed in cultured hippocampal neurons using viral vectors. Neuron 1998;20:855-67.
10. Jiao S, Cheng L, Wolff JA, Yang NS. Particle bombardment-mediated gene transfer and expression in rat brain tissues. Biotechnology (NY) 1993;11:497-502.
11. Kaech S, Kim JB, Cariola M, Ralston E. Improved lipid-mediated gene transfer into primary cultures of hippocampal neurons. Brain Res Mol Brain Res 1996;35:344-8.
12. Kaltschmidt C, Kaltschmidt B, Baeuerle PA. Stimulation of ionotropic glutamate receptors activates transcription factor NF-κ B in primary neurons. Proc Natl Acad Sci USA 1995;92:9618-22.
13. Kaltschmidt B, Uherek M, Volk B, Baeuerle PA, Kaltschmidt C. Transcription factor NF-κB is activated in primary neurons by amyloid b peptides and in neurons surrounding early plaques from patients with Alzheimer disease. Proc Natl Acad Sci USA 1997;94:2642-7.
14. Kaltschmidt B, Uherek M, Wellmann H, Kaltschmidt C. Inhibition of NF-(B potentiates amyloid-(-mediated neuronal apoptosis. Proc Natl Acad Sci USA 1999;96:9409-14.
15. Klein RM, Wolf ED, Wu R, Sanford JC. High-velocity microprojectiles for delivering nucleic acids into living cells. Biotechnology 1992;24:384-6.
16. Liu YC, Chapman ER, Storm DR. Targeting of neuromodulin (GAP-43) fusion proteins to growth cones in cultured rat embryonic neurons. Neuron 1991;6:411-20.
17. Lo DC, McAllister AK, Katz LC. Neuronal transfection in brain slices using particle-mediated gene transfer. Neuron 1994;13:1263-8.
18. McAllister AK, Lo DC, Katz LC. Neurotrophins regulate dendritic growth in developing visual cortex. Neuron 1995;15:791-803.
19. Moriyoshi K, Richards LJ, Akazawa C, O'Leary DD, Nakanishi S. Labeling neural cells using adenoviral gene transfer of membrane-targeted GFP. Neuron 1996;16:255-60.
20. Sanford JC, Smith FD, Russell JA. Optimizing the biolistic process for different biological applications. Methods Enzymol 1993;217:483-509.
21. Stoppini L, Buchs PA, Muller D. A simple method for organotypic cultures of nervous tissue. J Neurosci Methods 1991;37:173-82.
22. 2+ influx regulates BDNF transcription by a CREB family transcription factor-dependent mechanism [published erratum appears in Neuron 1998;20(6):1297]. Neuron 1998;20:709-26.
23. Xia Z, Dudek H, Miranti CK, Greenberg ME. Calcium influx via the NMDA receptor induces immediate early gene transcription by a MAP kinase/ERK-dependent mechanism. J Neurosci 1996;16:5425-36.
24. 2+ flux. Biochem Biophys Res Commun 1997;234:695-700.