Granzyme B; the chalk-mark of a cytotoxic lymphocyte

Journal of Translational Medicine

Volumn 2, Issue , 2004, Pages

  Waterhouse, Nigel J ^a   Sedelies, Karin A ^a   Clarke, Chris J P ^a  

^a PETER MACCALLUM CANCER CENTRE   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

CHROMIUM 51; GRANZYME B;

ANALYTICAL ERROR; APOPTOSIS; ARTICLE; CELL KILLING; CORRELATION ANALYSIS; CYTOLYSIS; CYTOTOXIC LYMPHOCYTE; DEGRANULATION; EFFECTOR CELL; ENZYME ASSAY; ENZYME LINKED IMMUNOSPOT ASSAY; ENZYME RELEASE; INTERMETHOD COMPARISON; MEASUREMENT; SYNAPSE; TARGET CELL;

EID: 18144379566     PISSN: 14795876     EISSN: None     Source Type: Journal    
DOI: 10.1186/1479-5876-2-36     Document Type: Article

References (19)

1. Waterhouse NJ, Trapani JA: CTL: Caspases Terminate Life, but that's not the whole story. Tissue Antigens 2002, 59: 175-183.
2. Waterhouse NJ, Clarke CJ, Sedelies KA, Teng MW, Trapani JA: Cytotoxic lymphocytes; instigators of dramatic target cell death. Biochem Pharmacol 2004, 68:1033-1040.
3. Roden MM, Lee KH, Panelli MC, Marincola FM: A novel cytolysis assay using fluorescent labeling and quantitative fluorescent scanning technology. J Immunol Methods 1999, 226:29-41.
4. Burrows SR, Suhrbier A, Khanna R, Moss DJ: Rapid visual assay of cytotoxic T-cell specificity utilizing synthetic peptide induced T-cell-T-cell killing. Immunology 1992, 76:174-175.
5. Goldberg JE, Sherwood SW, Clayberger C: A novel method for measuring CTL and NK cell-mediated cytotoxicity using annexin V and two-color flow cytometry. J Immunol Methods 1999, 224:1-9.
6. Jerome KR, Sloan DD, Aubert M: Measurement of CTL-induced cytotoxicity: the caspase 3 assay. Apoptosis 2003, 8: 563-571.
7. Liu L, Chahroudi A, Silvestri G, Wernett ME, Kaiser WJ, Safrit JT, Komoriya A, Altman JD, Packard BZ, Feinberg MB: Visualization and quantification of T cell-mediated cytotoxicity using cell-permeable fluorogenic caspase substrates. Nat Med 2002, 8:185-189.
8. Okano M, Purtilo DT: Simple assay for evaluation of Epstein-Barr virus specific cytotoxic T lymphocytes. J Immunol Methods 1995, 184:149-152.
9. Rininsland FH, Helms T, Asaad RJ, Boehm BO, Tary-Lehmann M: Granzyme B ELISPOT assay for ex vivo measurements of T cell immunity. J Immunol Methods 2000, 240:143-155.
10. Zagury D: Direct analysis of individual killer T cells: susceptibility of target cells to lysis and secretion of hydrolytic enzymes by CTL. Adv Exp Med Biol 1982, 146:149-169.
11. Shafer-Weaver K, Sayers T, Strobl S, Derby E, Ulderich T, Baseler M, Malyguine A: The Granzyme B ELISPOT assay: an alternative to the 51Cr-release assay for monitoring cell-mediated cytotoxicity. J Transl Med 2003, 1:14.
12. Trapani JA, Smyth MJ: Functional significance of the perforin/granzyme cell death pathway. Nat Rev Immunol 2002, 2:735-747.
13. Wowk ME, Trapani JA: Cytotoxic activity of the lymphocyte toxin granzyme B. Microbes Infect 2004, 6:752-758.
14. Pinkoski MJ, Waterhouse NJ, Heibein JA, Wolf BB, Kuwana T, Goldstein JC, Newmeyer DD, Bleackley RC, Green DR: Granzyme B-mediated apoptosis proceeds predominantly through a Bcl-2-inhibitable mitochondrial pathway. J Biol Chem 2001, 276:12060-12067.
15. Kimberly A Shafer-Weaver Thomas Sayers, Douglas B Kuhns, Susan L Strobl, Mark W Burkett, Michael Baseler and Anatoli Malyguine: Evaluating the cytotoxicity of innate immune effector cells using the GrB ELISPOT assay. Journal of Translational Medicine 2004, 2: 31.
16. Smyth MJ, Thia KY, Street SE, MacGregor D, Godfrey DI, Trapani JA: Perforin-mediated cytotoxicity is critical for surveillance of spontaneous lymphoma. J Exp Med 2000, 192: 755-760.
17. Smyth MJ, Thia KY, Cretney E, Kelly JM, Snook MB, Forbes CA, Scalzo AA: Perforin is a major contributor to NK cell control of tumor metastasis. J Immunol 1999, 162:6658-6662.
18. Smyth MJ, Street SE, Trapani JA: Cutting edge: granzymes A and B are not essential for perforin-mediated tumor rejection. J Immunol 2003, 171:515-518.
19. Bladergroen BA, Meijer CJ, ten Berge RL, Hack CE, Muris JJ, Dukers DF, Chott A, Kazama Y, Oudejans JJ, van Berkum O, Kummer JA: Expression of the granzyme B inhibitor, protease inhibitor 9, by tumor cells in patients with non-Hodgkin and Hodgkin lymphoma: a novel protective mechanisfm for tumor cells to circumvent the immune system? Blood 2002, 99:232-237.