Imaging Ca2+ concentration changes at the secretory vesicle surface with a recombinant targeted cameleon

Current Biology

Volumn 9, Issue 16, 1999, Pages 915-918

  Emmanouilidou, Evaggelia ^a   Teschemacher, Anja G ^a   Pouli, Aristea E ^a   Nicholls, Linda I ^a   Seward, Elizabeth P ^a   Rutter, Guy A ^a  

^a UNIVERSITY OF BRISTOL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CALCIUM; CALMODULIN; CELL FUSION; CELL MEMBRANE TRANSPORT; CELL SURFACE; CYTOSOL; EXOCYTOSIS; FLUORESCENCE; GREEN FLUORESCENT PROTEIN; MEMBRANE FUSION; PHOGRIN; PROBE; PROTEIN BINDING; PROTEIN LOCALIZATION; PROTEIN TARGETING; RECOMBINANT PROTEIN; SOLUTE CONCENTRATION;

BACTERIAL PROTEIN; CALCIUM; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; MEMBRANE PROTEIN; PHOTOPROTEIN; PROTEIN TYROSINE PHOSPHATASE; PTPRN2 PROTEIN, HUMAN; YELLOW FLUORESCENT PROTEIN, BACTERIA;

ANIMAL; ARTICLE; CELL GRANULE; CELL LINE; CELL STRAIN; FLUORESCENCE MICROSCOPY; METABOLISM; METHODOLOGY; RAT;

ANIMALS; BACTERIAL PROTEINS; CALCIUM; CELL LINE; CYTOPLASMIC GRANULES; GREEN FLUORESCENT PROTEINS; LUMINESCENT PROTEINS; MEMBRANE GLYCOPROTEINS; MEMBRANE PROTEINS; MICROSCOPY, FLUORESCENCE; PC12 CELLS; PROTEIN-TYROSINE-PHOSPHATASE; RATS; RECOMBINANT FUSION PROTEINS;

EID: 0033606891     PISSN: 09609822     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0960-9822(99)80398-4     Document Type: Article

References (17)

1. Martin TFJ: Stages of regulated exocytosis. Trends Cell Biol 1997, 7:271-276.
2. 2+ based on green fluorescent proteins and calmodulin. Nature 1997, 388:882-887.
3. Wasmeier C, Hutton JC: Molecular cloning of phogrin, a protein-tyrosine phosphatase homologue localized to insulin secretory granule membranes. J Biol Chem 1996, 271:18161-18170.
4. Papini E, Rossetto O, Cutler DF: Vesicle-associated membrane protein (VAMP)/synaptobrevin-2 is associated with dense core secretory granules in PC12 neuroendocrine cells. J Biol Chem 1995, 270:1332-1336.
5. Tooze SA, Huttner WB: Cell-free protein sorting to the regulated and constitutive secretory pathways. Cell 1990, 60:837-847.
6. Pouli AE, Emmanouilidou E, Zhao C, Wasmeier C, Hutton JC, Rutter GA: Secretory-granule dynamics visualized in vivo with a phogrin green fluorescent protein chimaera. Biochem J 1998, 333:193-199.
7. 2+ in the vicinity of secretory granules. Biochem J 1998, 330:1399-1404.
8. 2+-ATPase inhibitor, thapsigargin. Agents and Actions 1990, 29:8-15.
9. 2+ channel is functionally coupled to the exocytotic machinery. Proc Natl Acad Sci USA 1999, 96:248-253.
10. 2+ release from internal stores controls exocytosis in pituitary gonadotrophs. Neuron 1997, 18:121-132.
11. 2+ channels are present in secretory granules and are transiently translocated to the plasma membrane during regulated exocytosis. J Biol Chem 1996, 271:30096-30104.
12. 2+ from single isolated pancreatic zymogen granules. Cell 1996, 84:473-480.
13. 2+ oscillations in exocrine cells evoked by agonists and inositol trisphosphate. Cell 1993, 74:661-668.
14. Su XD, Taddei N, Stefani M, Ramponi G, Nordlund P: The crystal structure of a low-molecular-weight phosphotyrosine protein phosphatase. Nature 1994, 370:575-578.
15. Ormo M, Cubitt AB, Kallio K, Gross LA, Tsien RY, Remington SJ: Crystal structure of the Aequorea victoria green fluorescent protein. Science 1996, 273:1392-1395.
16. 2+ indicator. J Biol Chem 1994, 269:10141-10149.
17. Denk W, Svoboda K: Photon upmanship: Why multiphoton imaging is more than a gimmick. Neuron 1997, 18:351-357.