Conditioned medium from keloid keratinocyte/keloid fibroblast coculture induces contraction of fibroblast-populated collagen lattices

British Journal of Dermatology

Volumn 152, Issue 4, 2005, Pages 639-645

  Mukhopadhyay, A ^a   Tan, E K J ^a   Khoo, Y T A ^a   Chan, S Y ^a   Lim, I J ^a   Phan, T T ^a  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

smooth muscle actin; Epithelial mesenchymal interactions; Fibroblast populated collagen lattice; Keloid scars; Transforming growth factor

Indexed keywords

ALPHA SMOOTH MUSCLE ACTIN; COLLAGEN; NEUTRALIZING ANTIBODY; TRANSFORMING GROWTH FACTOR BETA;

ARTICLE; CELL CULTURE; CELL INTERACTION; CELL POPULATION; CELL PROLIFERATION; CONTROLLED STUDY; EPITHELIUM CELL; FIBROBLAST; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; KELOID; KERATINOCYTE; MESENCHYME; PARACRINE SIGNALING; PATHOGENESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; SKIN INJURY;

ACTINS; ANTIBODIES; CELLS, CULTURED; COCULTURE TECHNIQUES; COLLAGEN; CULTURE MEDIA, CONDITIONED; EPITHELIUM; FIBROBLASTS; HUMANS; IMMUNOHISTOCHEMISTRY; KELOID; KERATINOCYTES; MODELS, BIOLOGICAL; TRANSFORMING GROWTH FACTOR BETA;

EID: 17644422190     PISSN: 00070963     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1365-2133.2005.06545.x     Document Type: Article

References (44)

1. Poochareon VN, Herman B. New therapies for the management of keloids. J Croniofac Surg 2003; 14:654-7.
2. Kozak LJ, Bacon WD, Krzyzanowski M, Wojtyniak B. Hospital use in Poland and the United States. In: U.S. Department of Health and Human Services Publication No. (PHS) 88-1478. Vital and Health Statistics, Series 5, no. 2. Washington DC: Goverment Printing Office, 1988.
3. Bitterman PB, Henke CA. Fibroproliferative disorders. Chest 1991; 99:S81-4.
4. Tredget EE, Shankowsky HA, Taerum TV et al. The role of inhalation injury in burn trauma: a Canadian experience. Ann Surg 1990; 212:720-7.
5. Engrav LH, Covey MH, Dutcher KD et al. Impairment, time out of school, and time off from work after burns. Plost Reconstr Surg 1987; 79:927-32.
6. Cohen IK, Peacock EE. Keloids and hypertrophic scars. In: Plastic Surgery (McCarthy JG, ed). Philadelphia: Saunders, 1990; 732-47.
7. Ladin D, Garner WL, Smith DS Jr. Excessive scarring as a consequence of healing. Wound Repair Regen 1995; 3:6-14.
8. Sayah DN, Chia S, Shaw WW et al. Downregulation of apoptosis-related genes in keloid tissues. J Surg Res 1999; 87:209-16.
9. Chodon T, Sugihara T, Igawa H et al. Keloid-derived fibroblasts are refractory to Fas-mediated apoptosis and neutralization of autocrine transforming growth factor-β 1 can abrogate this resistance. Am J Pathol 2000; 157:1661-9.
10. Tuan TL, Nitcher LS. The molecular basis of keloid and hypertrophic scar formation. Mol Med Today 1998; 4:19-24.
11. Niessen FB, Spauwen PHM, Schalkwijk J, Ron M. On the nature of hypertrophic scars and keloids: a review. Plost Reconstr Surg 1999; 104:1435-58.
12. Kim WJH, Levinson H, Gittes GK, Longaker MT. Mechanisms in keloid biology. In: Scarless Wound Healing (Garg HG, Longaker MT, eds). New York: Marcel Dekker, 2000; 161-71.
13. Lim IJ, Phan TT, Song C et al. Investigation of the influence of keloid-derived keratinocytes on fibroblast growth and proliferation in vitro. Plast Reconstr Surg 2001; 107:797-808.
14. Funayama E, Chodon T, Oyama A, Sugihara T. Keratinocytes promote proliferation and inhibit apoptosis of the underlying fibroblasts: an important role in the pathogenesis of keloid. J Invest Dermatol 2003; 111:1326-31.
15. Xia W, Phan TT, Lim IJ et al. Complex epithelial-mesenchymal interactions modulate transforming growth factor-β expression in keloid-derived cells. Wound Repair Regen 2004; 12:546-56.
16. Lim IJ, Phan TT, Bay BH et al. Fibroblasts co-cultured with keloid keratinocytes: normal fibroblasts secrete collagen in keloidlike manner. Am J Physiol Cell Physiol 2002; 283:C212-22.
17. Lim IJ, Phan TT, Tan EK et al. Synchronous activation of ERK and phosphotidylinositol 3-kinase pathways is required for collagen and extracellular matrix production in keloids. J Biol Chem 2003; 278:40851-8.
18. Tomasek JJ, Gabbiani G, Hinz B et al. Myofibroblasts and mechanoregulation of connective tissue remodeling. Nat Rev Mol Cell Biol 2002; 3:349-63.
19. Hurle JM, Ganan Y. Interdigital tissue chondrogenesis induced by surgical removal of the ectoderm in the embryonic chick leg bud. J Embryol Exp Morph 1986; 94:231-44.
20. Kamamoto F, Paggiaro AO, Rodas A et al. A wound contraction experimental model for studying keloids and wound healing modulators. Artif Organs 2003; 27:701-5.
21. Spyrou GE, Naylor IL. The effect of basic fibroblast growth factor on scarring. Br J Plast Surg 2002; 55:275-82.
22. Smith P, Mosiello G, Deluca L et al. TGF-β2 activates proliferative scar fibroblasts. J Surg Res 1999; 82:319-23.
23. Kim ES, Kim RS, Ren RF et al. Transforming growth factor-β inhibits apoptosis induced by β-amyloid peptjde fragment 25-35 in cultured neuronal cells. Mol Brain Res 1998; 62:122-30.
24. Genestier L, Kasibhatla S, Brunner T, Green DR. Transforming growth factor-β inhibits Fas ligand expression and subsequent activation-induced cell death in T cells via downregulation of c-Myc. J Exp Med 1999; 189:231-9.
25. 1. Am J Respir Cell Mol Biol 1999; 21:658-65.
26. Chin BY, Petrache I, Choi AM, Choi ME. Transforming growth factor beta 1 rescues serum deprivation-induced apoptosis via the mitogen-activated protein kinase (MAPK) pathway in macrophages. J Biol Chem 1999; 274:11362-8.
27. Bell E, Ivarsson B, Merril C. Production of a tissue-like structure by contraction of collagen lattices by human fibroblasts of different proliferative potentials in vitro. Proc Natl Acad Sci USA 1979; 76: 1274-8.
28. Harris AK, Stopak D, Wild P. Fibroblast traction as a mechanism for collagen morphogenesis. Nature 1981; 290:249-51.
29. Grinell F. Fibroblasts: myofibroblasts and wound contraction. Mini review on the cellular mechanism of diseases. J Cell Biol 1994; 124:401-4.
30. Ala-Kokko L, Rintala A, Savolainen ER. Collagen gene expression in keloids: analysis of collagen metabolism and type I, III, IV, and V procollagen mRNAs in keloid tissue and keloid fibroblast cultures. J Invest Dermatol 1987; 89:238-44.
31. Di Cesare PE, Cheung DT, Perelman N et al. Alteration of collagen composition and cross-linking in keloid tissues. Matrix 1990; 10:172-8.
32. Yang GP, Lim JI, Phan TT et al. From scarless wounds to keloids: molecular studies in wound healing. Wound Repair Regen 2003; 11:411-18.
33. Fusenig NE. Epithelial-mesenchymal interactions regulate keratinocyte growth and differentiation in-vitro. In: The Keratinocyte Handbook (Leigh I, Lane B, Watt F, eds). Cambridge: Cambridge University Press, 1994; 71-94.
34. Mass-Szabowski N, Shimotoyodome A, Fusenig NE. Keratinocyte growth regulation in fibroblast cocultures via a double paracrine mechanism. J Cell Sci 1999; 111:1843-53.
35. Mackenzie IC. Epithelial-mesenchymal interactions in the development and maintenance of epithelial tissues. In: The Keratinocyte Handbook (Leigh I, Lane B, Watt F, eds). Cambridge: Cambridge University Press, 1994; 234-57.
36. Border WA, Nobel NA. Transforming growth factor-beta in tissue fibrosis. N Engl J Med 1994; 331:1286-92.
37. Montesano R, Orci L. Transforming growth factor-β stimulates collagen-matrix contraction by fibroblasts: implications for wound healing. Proc Natl Acad Sci USA 1998; 85:4894-7.
38. Finesmith TH, Broadley KN, Davidson JM. Fibroblasts from wounds of different stages of repair vary in their ability to contract a collagen gel in response to growth factors. J Cell Physiol 1990; 144:99-107.
39. Derynck R, Zhang YE. Smad-dependent and Smad-independent pathways in TGF-β family signaling. Nature 2003; 425:577-84.
40. Sumiyoshi K, Nakao A, Setoguchi Y et al. Smads regulate collagen gel contraction by human dermal fibroblasts. Br J Dermatol 2003; 149:464-70.
41. Roy SG, Nozaki Y, Phan SH. Regulation of α-smooth muscle actin expression in myofibroblast differentiation from rat lung fibroblast. Int J Biochem Cell Biol 2001; 33:723-34.
42. Desmouliere A, Geinoz A, Gabbiani F, Gabbiani G. Transforming growth factor-beta1 induces alpha-smooth muscle actin expression in granulation tissue myofibroblasts and in quiescent and growing cultured fibroblasts. J Cell Biol 1993; 122:103-11.
43. Desmouliere A. Factors influencing myofibroblast differentiation during wound healing and fibrosis. Cell Biol Int 1995; 19:471-6.
44. Ronnov-Jessen L, Peterson OW. Induction of alpha smooth muscle actin by transforming growth factor-beta 1 in quiescent human breast gland fibroblasts. Implications for myofibroblast generation in breast neoplasia. Lab Invest 1993; 68:696-70.