Construction and expression of siRNA expression constructs targeting epidermal growth factor receptor

Chinese Journal of Medical Genetics

Volumn 22, Issue 2, 2005, Pages 146-150

  Kang, Chun Sheng ^a   Pu, Pei Yu ^a   Wang, Guang Xiu ^a   Dong, Lun ^a   Li, Yan He ^a   Wang, Hu ^a  

^a TIANJIN MEDICAL UNIVERSITY   (China)

Author keywords

Epidermal growth factor receptor; Expression; Glioma; Plasmid; Small interfering RNA

Indexed keywords

EPIDERMAL GROWTH FACTOR RECEPTOR; LIPOFECTAMINE; SMALL INTERFERING RNA;

ARTICLE; CANCER CELL; CONTROLLED STUDY; ENZYME ACTIVE SITE; GENE CONSTRUCT; GENE EXPRESSION; GENE TARGETING; GENE THERAPY; GENETIC TRANSFECTION; GLIOMA; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE; NUCLEOTIDE SEQUENCE; PLASMID; PROTEIN EXPRESSION; WESTERN BLOTTING;

BLOTTING, WESTERN; CELL LINE, TUMOR; FLUORESCENT ANTIBODY TECHNIQUE; HUMANS; MODELS, GENETIC; PLASMIDS; RECEPTOR, EPIDERMAL GROWTH FACTOR; RNA INTERFERENCE; RNA, SMALL INTERFERING; TRANSFECTION;

EID: 17244370857     PISSN: 10039406     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (12)

1. Wells A. EGF receptor. Int J Biochem Cell, 1999, 31: 637-643.
2. Mendelsohn J, Baselga J. The EGF receptor family as targets for cancer therapy. Oncogene,2000, 19: 6550-6565.
3. Testa JR, Bellacosa A. AKT plays a central role in tumorigenesis. Proc Natl Acad Sci USA, 2001, 98: 10983-10985.
4. Fire A, Xu S, Montgomery MK, et al. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature, 1998, 391: 806-811.
5. Zamore PD. RNA interference: listening to the sound of silence. Nat Struct Biol, 2001, 8: 746-751.
6. Hohjoh H. RNA interference (RNAi) induction with various types of synthetic oligonucleotide duplexes in cultured human cells. FEBS Lett, 2002, 521: 195-199.
7. Wang SL, Li F, Pu PY, et al. Establishment and characteristics of human brain malignant cell lines TJ899 and TJ905. Tianjin Med, 1996, 24: 416-418.
8. Myslinski E, Amé JC, Krol A, et al. An unusually compact external promoter for RNA polymerase III transcription of the human H1RNA gene. Nucleic Acids Res, 2001, 29: 2502-2509.
9. Brummelkamp TR, Bernards R, Agami R. Stable suppression of tumorigenicity by virus-mediated RNA interference. Cancer Cell, 2002, 2: 243-247.
10. Zhang L, Yang N, Mohamed-Hadley A, et al. Vector-based RNAi, a novel tool for isoform-specific knock-down of VEGF and anti-angiogenesis gene therapy of cancer. Biochem Biophy Res Commun, 2003, 303: 1169-1178.
11. Nagy P, Arndt-Jovin DJ, Jovin TM. Small interfering RNAs suppress the expression of endogenous and GFP-fused epidermal growth factor receptor (erbB1) and induce apoptosis in erbB1-overexpressing cells. Exp Cell Res, 2003, 285: 39-49.
12. Sorensen DR, Leirdal M, Sioud M. Gene silencing by systemic delivery of synthetic siRNAs in adult mice. J Mol Biol, 2003, 327: 761-766.