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Volumn 14, Issue 3, 2004, Pages 685-687

Peptidyl aldehydes as slow-binding inhibitors of dual-specificity phosphatases

Author keywords

[No Author keywords available]

Indexed keywords

ALDEHYDE; ENZYME INHIBITOR; PHOSPHATASE; UNCLASSIFIED DRUG; VACCINIA OPEN READING FRAME H1 PHOSPHATASE; VACCINIA OPEN READING FRAME H1 RELATED PHOSPHATASE;

EID: 1642534339     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2003.11.027     Document Type: Article
Times cited : (11)

References (20)
  • 18
    • 85030890760 scopus 로고    scopus 로고
    • Enzyme assays were performed as follows. A typical reaction (total volume 1.0 mL) contained 50 mM imidazole (pH=7.0), 1 mM EDTA, 0.05% (v/v) β-mercaptoethanol, 5% DMSO, 0.1-0.2 μM VH1 (or 0.5-1 μM VHR), and 0-2700 μM inhibitor. After incubation of the enzyme with the inhibitor for 1 h at room temperature, the reaction was initiated by the addition of 5 mM pNPP. The reaction progress was monitored at 405 nm on a Perkin-Elmer UV-Vis spectrophotometer
    • Enzyme assays were performed as follows. A typical reaction (total volume 1.0 mL) contained 50 mM imidazole (pH=7.0), 1 mM EDTA, 0.05% (v/v) β-mercaptoethanol, 5% DMSO, 0.1-0.2 μM VH1 (or 0.5-1 μM VHR), and 0-2700 μM inhibitor. After incubation of the enzyme with the inhibitor for 1 h at room temperature, the reaction was initiated by the addition of 5 mM pNPP. The reaction progress was monitored at 405 nm on a Perkin-Elmer UV-Vis spectrophotometer.
  • 19
    • 85030905244 scopus 로고    scopus 로고
    • note
    • 6 value was determined from the reactivation experiment as described in the text. All measurements were performed at room temperature.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.