Development of a novel method for isolating and detecting Cryptosporidium parvum from lean and fat beef carcass surfaces

Food Microbiology

Volumn 21, Issue 3, 2004, Pages 275-282

  Moriarty, E M ^a   McEvoy, J M ^a   Duffy, G ^a,b   Sheridan, J J ^a   Blair, I S ^b   McDowell, D A ^b  

^a ASHTOWN FOOD RESEARCH CENTRE   (Ireland)

^b UNIVERSITY OF ULSTER   (United Kingdom)

Author keywords

Beef; Cryptosporidium; Method; Pulsifier

Indexed keywords

CRYPTOSPORIDIUM; CRYPTOSPORIDIUM PARVUM;

EID: 1642424305     PISSN: 07400020     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.fm.2003.08.005     Document Type: Article

References (41)

1. Anderson, B.C., 1988. Gastric cryptosporidiosis of feeder cattle, beef cows, and dairy cows. Bovine Practitioner 23, 99-101.
2. Bukhari, Z., Smith, H.V., 1996. Detection of Cryptosporidium muris oocysts in the faeces of adult dairy cattle in Scotland. Vet. Rec. 138, 207-208.
3. Cacció, S., Homan, W., Camilli, R., Traldi, G., Kortbeek, T., Pozio, E., 2000. A microsatellite marker reveals population heterogeneity within human and animal genotypes of Cryptosporidium. J. Parasitol. 120, 237-244.
4. Casemore, D.P., Jessop, E.C., Douce, D., Jackson, F.B., 1986. Cryptosporidium plus Campylobacter: an outbreak in a semi-rural population. J. Hyg. Cambridge 96, 95-105.
5. Centre for Disease Control, 1982. Cryptosporidiosis: assessment of males with acquired immune deficiency syndrome (AIDS). Morbid Mortal Weekly Rep. 31, 589-592.
6. Current, W.L., Garcia, L.S., 1991. Cryptosporidiosis. Clin. Microbiol. Rev. 4, 325-358.
7. Deng, M.Q., Cliver, D.O., 1999. Cryptosporidium parvum studies with dairy products. Int. J. Food Microbiol. 46, 113-121.
8. Deng, M.Q., Cliver, D.O., 2000. Comparative detection of Cryptosporidium parvum oocysts from apple juice. Int. J. Food Microbiol. 54, 155-162.
9. DuPont, H.L., Chappell, C.L., Sterling, C.R., Okhuysen, P.C., Rose, J.B., Jakubowski, W., 1995. The infectivity of Cryptosporidium parvum in healthy volunteers. N. Engl. J. Med. 332, 854-855.
10. Ernst, J.V., Steward, T.B., Witlock, D.R., 1987. Quantitative determinations of coccidia oocysts in beef calves from coastal plain area of Georgia (USA). Vet. Parasitol. 23, 1-10.
11. Fayer, R., 1997. In: Fayer, R. (Ed.), Cryptosporidium and Cryptosporidiosis. CRC, New York, pp. 1-251.
12. Fung, D.Y.C., Sharpe, A.N., Hart, B.C., Liu, Y., 1998. The pulsifier: a new instrument for preparing food suspensions for microbiological analysis. J. Rapid Methods Auto. Microbiol. 6, 43-49.
13. Gomez-Bautista, M., Ortega-Mora, L.M., Tabares, E., Lopez-Rodas, V., Costas, E., 2000. Detection of infectious Cryptosporidium parvum oocysts in mussels (Mytilus galloprovincialis) and cockles (Cerastoderma edule). Appl. Environ. Microbiol. 66, 1866-1870.
14. Guyot, K., Gireaudot-Liepmann, M.F., Riveau-Ricard, I., Carbon, A., Lange, M., Delattre, J.M., Dei-Cas, E., 2000. Influence of US EPA 1622 method successive steps on the viability of Cryptosporidium oocysts. Water Sci. Technol. 41, 189-196.
15. Heine, J., Pohlenz, J.F.L., Moon, H.W., 1984. Enteric lesions and diarrhea in gnotobiotic calves monoinfected with Cryptosporidium species. J. Infect. Dis. 150, 768-775.
16. Hoxie, N.J., Davis, J.P., Vergeront, J.M., Nashold, R.D., Blair, K.A., 1997. Cryptosporidiosis-associated mortality following a massive waterborne outbreak in Milwaukee, Wisconsin. Am. J. Public Health 87, 2032-2035.
17. Hsu, B.M., Huang, C., Hsu, Y.F., Jiang, G.Y., Hsu, C.L.L., 2001. Evaluation of two concentration methods for detecting Giardia and Cryptosporidium in water. Water Res. 35, 419-424.
18. Laberge, I., Ibrahim, A., Barta, J.R., Griffiths, M.W., 1996. Detection of Cryptosporidium parvum in raw milk by PCR and oligonucleotide probe hybridization. Appl. Environ. Microbiol. 62, 3259-3264.
19. LeChevallier, M.W., Norton, W.D., Siegel, J.E., Abbaszadegan, M., 1995. Evaluation of the immunofluorescence procedure for detection of Giardia cysts and Cryptosporidium oocysts in water. Appl. Environ. Microbiol. 61, 690-697.
20. Mann, E.D., Nayar, G.P.S., Koschik, C., 1986. Infection with Cryptosporidium spp. in humans and cattle in Manitoba. Can. J. Vet. Rec. 50, 174-178.
21. McCuin, J.M., Bukhari, Z., Sobrinho, J., Clancy, J.L., 2001. Recovery of Cryptosporidium oocysts and Giardia cysts from source water concentrates using immunomagnetic separation. J. Microbiol. Methods 45, 69-76.
22. McEvoy, J.M., Doherty, A.M., Sheridan, J.J., Blair, I.S., Garvey, P., McGuire, L., Blair, I.S., McDowell, D.A., 2003a. The prevalence and spread of Escherichia coli O157:H7 at a commercial beef abattoir. J. Appl. Microbiol. 95, 256-266.
23. McEvoy, J.M., Moriarty, E.M., Duffy, G., Sheridan, J.J., 2003b. The role of beef in the transmission of Cryptosporidium parvum. In: Proceedings of the Cryptosporidium Parvum in the Food and Water Chain. The National Food Centre, Ashtown, Teagasc, Dublin 15, 30th January 2003, pp. 25-25.
24. Millard, P.S., Gensheimer, K.F., Addiss, D.G., Sosin, D.M., Beckett, G.A., Houck-Janoski, A., Hudson, A., 1994. An outbreak of cryptosporidiosis from freshly-pressed apple cider. J. Appl. Med. Assoc. 272, 1592-1596.
25. Nichols, G., Thom, B.T., 1985. Food poisoning caused by Cryptosporidium: a load of tripe. CDR 4, 3.
26. Nime, F.A., Burek, J.D., Page, D.L., Holscher, M.A., Yardley, J.H., 1976. Acute enterocolitis in a human being infected with the protozoan Cryptosporidium. Gastroenterology 70, 592-598.
27. Nouri, M., Toroghi, R., 1991. Asymptomatic cryptosporidiosis in cattle and humans in Iran. Vet. Rec. 128, 358-359.
28. Ortega, Y.R., Roxas, C.R., Gilman, R.H., Miller, N.J., Cabrera, L., Taquiri, C., Sterling, C.R., 1997. Isolation of Cryptosporidium parvum and Cyclospora cayetanensis from vegetables collected in markets of an endemic region in Peru. Am. J. Trop. Med. Hyg. 57, 683-686.
29. Palumbo, S.A., Klein, P., Capra, J., Eblen, S., Miller, A.J., 2000. Comparison of excision and swabbing sampling methods to determine the microbiological quality of swine carcass surfaces. Food Microbiol. 16, 459-464.
30. Payne, R.W., 1993. GENSTAT 5.0 Release 3 (1) Procedure Library Manual. Lawes Agricultural Trust, Rothamsted Experimental Station, Hertfordshire, UK.
31. Reynolds, D.T., Slade, R.B., Slykes, N.J., Jonas, A., Fricker, C.R., 1999. Detection of Cryptosporidium oocysts in water: techniques for generating precise recovery data. Appl. Microbiol. 87, 804-813.
32. Robertson, L.J., Gjerde, B., 2001. Occurrence of parasites on fruits and vegetables in Norway. J. Food Prot. 64, 1793-1798.
33. Rose, J.B., 2000. Occurrence and control of Cryptosporidium in drinking water. In: McFeters, G.A. (Ed.), Advances in Drinking Water Microbiology Research. Madison, Science Tech., pp. 294-321.
34. Rose, J.B., Slifko, T.R., 1999. Giardia, Cryptosporidium, and Cyclospora and their impact on food: a review. J. Food Prot. 62, 1059-1070.
35. Rose, J.B., Kayed, D., Madore, M.S., Gerba, C.P., Arrowood, M., Sterling, C.R., Riggs, M.W., 1988. Methods for the recovery of Giardia and Cryptosporidium from environmental waters and their comparative occurrence. In: Wallis, P.M., Hammond, B.R. (Eds.), Advances in Giardia Research. University of Calgary Press, Calgary, Alberta, pp. 205-209.
36. Rose, J.B., Landeen, L.K., Riley, K.R., Gerba, C.P., 1989. Evaluation of immunofluorescence techniques for detection of Cryptosporidium oocysts and Giardia from environmental samples. Appl. Environ. Microbiol. 55, 3189-3196.
37. Sanford, S.E., Josephson, G.K.A., 1982. Bovine Cryptosporidiosis: clinical and pathological findings in forth-two scouring neonatal calves. Can. Vet. J. 23, 343-347.
38. Sharpe, A.N., Hearn, E.M., Kovacs-Nolan, J., 2000. Comparison of membrane filtration rates and hydrophobic grid membrane filter Coliform and E. coli counts in food suspensions using paddle-type and pulsifier sample preparations procedures. J. Food Prot. 63, 126-130.
39. Shepherd, K.M., Wyn-Jones, A.P., 1996. An evaluation of methods for the simultaneous detection of Cryptosporidium oocysts and Giardia cysts from water. Appl. Environ. Microbiol. 62, 1317-1322.
40. Sterling, C.R., Seegar, K., Sinclair, N.A., 1986. Cryptosporidium as a causative agent of traveler's diarrhea. J. Infect. Dis. 153, 380-381.
41. Villacorta, I., 1991. Cryptosporidium parvum in cattle, sheep and pigs in Galicia (N.W.Spain). Vet. Parasitol. 38, 249-253.