A new kinetic model of recombinant β-galactosidase from Kluyveromyces lactis for both hydrolysis and transgalactosylation reactions

Biochemical and Biophysical Research Communications

Volumn 316, Issue 3, 2004, Pages 738-743

  Kim, Chang Sup ^a   Ji, Eun Su ^a   Oh, Deok Kun ^a,b  

^a Sejong University   (South Korea)

^b Hanbat National University   (South Korea)

Author keywords

Galactosidase; Kinetic model; Kluyveromyces lactis; Lactose hydrolysis; Transgalactosylation

Indexed keywords

BETA GALACTOSIDASE; GALACTOSE; GLUCOSE;

ARTICLE; CHEMICAL MODIFICATION; CHEMICAL REACTION; CONCENTRATION RESPONSE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME INHIBITION; HYDROLYSIS; INHIBITION KINETICS; KINETICS; KLUYVEROMYCES; NONHUMAN; PRIORITY JOURNAL; THEORETICAL MODEL; TRANSGALACTOSYLATION;

BETA-GALACTOSIDASE; DOSE-RESPONSE RELATIONSHIP, DRUG; ESCHERICHIA COLI; GALACTOSE; GLUCOSE; HYDROLYSIS; KINETICS; KLUYVEROMYCES; LACTOSE; MODELS, CHEMICAL; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; TIME FACTORS;

KLUYVEROMYCES; KLUYVEROMYCES LACTIS;

EID: 1642416611     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbrc.2004.02.118     Document Type: Article

References (19)

1. Gekas V., Lopez-Leiva M. Hydrolysis of lactose: a literature review. Process Biochem. 20:1985;2-12.
2. Cavaille D., Combes D. Characterization of β-galactosidase from Kluyveromyces lactis. Biotechnol. Appl. Biochem. 22:1995;55-64.
3. Zhou Q.Z.K., Chen X.D. Effects of temperature and pH on the catalytic activity of the immobilized β-galactosidase from Kluyveromyces lactis. Biochem. Eng. J. 9:2001;33-40.
4. Fontes E.A.F., Passos F.M.L., Passos F.J.V. A mechanistical mathematical model to predict lactose hydrolysis by β-galactosidase in a permeabilized cell mass of Kluyveromyces lactis: validity and sensitivity analysis. Process Biochem. 37:2001;267-274.
5. Yang S.T., Okos M.R. A new graphical method for determining parameters in Michaelis-Menten-type kinetics for enzymatic lactose hydrolysis. Biotechnol. Bioeng. 34:1989;763-773.
6. Carrara C.R., Rubiolo A.C. Determination of kinetics parameters for free and immobilized β-galactosidase. Process Biochem. 31:1996;243-248.
7. Santos M.L., Ladero M., García-Ochoa F. Kinetic modeling of lactose hydrolysis by a β-galactosidase from Kluyveromyces fragilis. Enzyme Microb. Technol. 22:1998;558-567.
8. Zhou Q.Z., Chen X.D., Li X. Kinetics of lactose hydrolysis by β-galactosidase of Kluyveromyces lactis immobilized on cotton fabric. Biotechnol. Bioeng. 81:2003;127-133.
9. Iwasaki K., Nakajima M., Nakao S. Galacto-oligosaccharide production from lactose by an enzymic batch reaction using β-galactosidase. Process Biochem. 31:1996;69-76.
10. Boon M.A., Janssen A.E., van der Padt A. Modeling and parameter estimation of the enzymatic synthesis of oligosaccharides by β-galactosidase from Bacillus circulans. Biotechnol. Bioeng. 64:1999;558-567.
11. Kim C.S., Ji E.S., Oh D.K. Expression and characterization of Kluyveromyces lactis β-galactosidase in Escherichia coli. Biotechnol. Lett. 25:2003;1769-1774.
12. Mendes P., Kell D.B. Non-linear optimization of biochemical pathways: applications to metabolic engineering and parameter estimation. Bioinformatics. 14:1998;869-883.
13. Mulquiney P.J., Kuchel P.W. Modelling Metabolism with Mathematica. 2003;CRC Press, Florida.
14. Pocker Y., Janjic N. Origin of viscosity effects in carbonic anhydrase catalysis: kinetic studies with bulky buffers at limiting concentrations. Biochemistry. 27:1988;4114-4120.
15. Demchenko A.P., Ruskyn O.I., Saburova E.A. Kinetics of the lactate dehydrogenase reaction in high-viscosity media. Biochim. Biophys. Acta. 998:1989;196-203.
16. Miranda M., Murado M.A. Mass transfer control of enzymatic hydrolysis of polysaccharides by glucoamylase. Enzyme Microb. Technol. 13:1991;142-147.
17. Lopez-Leiva M.H., Guzman M. Formation of oligosaccharides during enzymic hydrolysis of milk whey permeates. Process Biochem. 30:1995;757-762.
18. Mahoney R.R. Galactosyl-oligosaccharide formation during lactose hydrolysis: a review. Food Chem. 63:1998;147-154.
19. Rustom I.Y.S., Foda M.I., Lopez-Leiva M.H. Formation of oligosaccharides from whey UF-permeate by enzymatic hydrolysis: analysis of factors. Food Chem. 62:1998;141-147.