A computer-controlled spraying-freezing apparatus for millisecond time- resolution electron cryomicroscopy

Journal of Structural Biology

Volumn 121, Issue 3, 1998, Pages 306-313

  White, H D ^a   Walker, M L ^b   Trinick, J ^b  

^a EASTERN VIRGINIA MEDICAL SCHOOL   (United States)

^b UNIVERSITY OF LEEDS   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; COMPUTER; ELECTRON MICROSCOPY; INSTRUMENTATION; MACROMOLECULE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN ASSEMBLY; PROTEIN BINDING; PROTEIN PROTEIN INTERACTION;

EID: 0031880854     PISSN: 10478477     EISSN: None     Source Type: Journal    
DOI: 10.1006/jsbi.1998.3968     Document Type: Article

References (20)

1. Bailey A. G. Electrostatic Spraying of Liquids. 1988.
2. Berriman J., Unwin N. Analysis of transient structures by cryo-microscopy combined with rapid mixing of spray droplets. Ultramicroscopy. 56:1994;241-252.
3. Collison, W. E. 1935, Inhalation Therapy T.
4. Craig R., Alamo L., Padron R. Structure of the myosin filaments of relaxed and rigor vertebrate striated muscle studied by rapid freezing electron microscopy. J. Mol. Biol. 228:1992;474-487.
5. Davies A., Hudson N., Pirie L. A novel ultrasonic aerosol generator. Med. Eng. Phys. 17:1995;387-389.
6. Dubochet J., Adrian M., Chang J. J., Homo J. C., Lepault J., McDowall A. W., Schultz P. Cryo-electron microscopy of vitrified specimens. Quart. Rev. Biophys. 21:1988;129-228.
7. Heuser J. E. Structure of the myosin crossbridge lattice in insect flight-muscle. J. Mol. Biol. 169:1983;123-154.
8. Hirose K., Lenart T. D., Murray J. M., Franzini-Armstrong C., Goldman Y. E. Flash and smash: Rapid freezing of muscle fibres activated by photolysis of caged ATP. Biophys. J. 65:1993;397-408.
9. Huxley H. E. Electron microscope studies of natural and synthetic protein filaments from striated muscle. J. Mol. Biol. 7:1963;281-308.
10. Huxley H. E. The mechanism of muscle contraction. Science. 164:1969;1356-1366.
11. Lepault J., Booy F. P., Dubochet J. Electron microscopy of frozen biological suspensions. J. Microsc. 129:1982;89-102.
12. Liu B. Y. H., Lee K. W. An aerosol generator of high stability. Am. Indust. Hyg. Assoc. J. 36:1975;861-865.
13. Mayer E., Astl G. Limits of cryofixation as seen by Fourier transform infrared spectroscopy of metmyoglobin azide and carbonyl hemoglobin in vitrified and freeze-concentrated aqueous solution. Ultramicroscopy. 45:1992;185-197.
14. Menetret J. F., Hofmann W., Schroder R. R., Rapp G., Goody R. S. Time-resolved cryoelectron microscopic study of the dissociation of actomyosin induced by photolysis of photolabile nucleotides. J. Mol. Biol. 219:1991;139-144.
15. Moffat K., Henderson R. Freeze trapping of reaction intermediates. Curr. Opin. Struct. Biol. 5:1995;656-663.
16. Rayment I., Holden H. M., Whittaker M., Yohn C. B., Lorenz M., Holmes K. C., Milligan R. A. Structure of the actin-myosin complex and its implications for muscle-contraction. Science. 261:1993;58-65.
17. Sosa H., Popp D., Ouyang G., Huxley H. E. Ultrastructure of skeletal-muscle fibers studied by a plunge quick-freezing method - myofilament lengths. Biophys. J. 67:1994;283-292.
18. Trinick J., Cooper J. Concentration of solutes during preparation of aqueous suspensions for cryo-electron microscopy. J. Microsc. 159:1990;215-222.
19. Walker M., White H., Belknap B., Trinick J. Electron cryo-microscopy of the acto-myosin-S1 complex during steady state ATP hydrolysis. Biophys. J. 66:1994;1563-1572.
20. Walker M., Trinick J., White H. Millisecond time resolution electron cryo-microscopy of the M-ATP transient kinetic state of the acto-myosin ATPase. Biophys. J. 68:1995;87s-91s.