Evaluation of different methods to determine the loading of proteins in PLGA microspheres

Pharmazie

Volumn 59, Issue 3, 2004, Pages 232-233

  Zheng, C H ^a   Liang, Wenquan Q ^a   Yu, H Y ^a   Chen, H L ^a  

^a ZHEJIANG UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ACETONITRILE; MICROSPHERE; POLYGLACTIN;

ARTICLE; ENCAPSULATION; EXTRACTION; HYDROLYSIS; PROTEIN ANALYSIS; PROTEIN DETERMINATION; SOLVENT EXTRACTION;

ACETONITRILES; DRUG COMPOUNDING; LACTIC ACID; METHYLENE CHLORIDE; MICROSPHERES; PARTICLE SIZE; POLYGLYCOLIC ACID; POLYMERS; PROTEINS; SERUM ALBUMIN, BOVINE; SOLUBILITY; SOLVENTS;

EID: 1642299546     PISSN: 00317144     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (11)

1. Castellanos IJ, Cruz G, Crespo R et al. (2002) Encapsulation-induced aggregation and loss in activity of γ-chymotrypsin and their prevention. J Control Release 81: 307-319.
2. Diwan M, Park TG (2001) Pegylation enhances protein stability during encapsulation in PLGA microspheres. J Control Release 73: 233-244.
3. Gutierro I, Hernandez RM, Igartua M et al. (2002) Influence of dose and immunization route on the serum IgG antibody response to BSA loaded PLGA microspheres. Vaccine 20: 2181-2190.
4. Kang F, Singh J (2001) Effect of additives on the release of a model protein from PLGA microspheres. AAPS Pharm Sci Tech 2: 230-239.
5. Lam XM, Duenas ET, Daugherty AL et al. (2000) Sustained release of recombinant human insulin-like growth factor-I for treatment of diabetes. J Control Release 67: 281-292.
6. Li YP, Pei YY, Zhang XY et al. (2001) PEGylated PLGA nanoparticles as protein carriers: synthesis, preparation and biodistribution in rats. J Control Release 71: 203-211.
7. Prior S, Gamazo C, Irache JM et al. (2000) Gentamicin encapsulation in PLA/PLGA microspheres in view of treating Brucella infections. Int J Pharm 196: 115-125.
8. Ravivarapu HB, Burton K, Deluca PP (2000) Polymer and microsphere blending to alter the release of a peptide from PLGA microspheres. Eur J Pharm Biopharm 50: 263-270.
9. Rosas JE, Hernandez RM, Gascon AR et al. (2001) Biodegradable PLGA microspheres as a delivery system for malaria synthetic peptide SPf 66. Vaccine 19: 4445-4451.
10. Tuncay M, Calis S, Kas HS et al. (2000) Diclofenac sodium incorporated PLGA (50:50) microspheres: formulation considerations and in vitro/in vivo evaluation. Int J Pharm 195: 179-188.
11. Yang YY, Chung TS, Ng NP (2001) Morphology, drug distribution, and in vitro release profiles of biodegradable polymeric microspheres containing protein fabricated by double-emulsion solvent extraction/evaporation method. Biomaterials 22: 231-241.