Fed-batch xylitol production with two recombinant Saccharomyces cerevisiae strains expressing XYL1 at different levels, using glucose as a cosubstrate: A comparison of production parameters and strain stability

Biotechnology and Bioengineering

Volumn 54, Issue 4, 1997, Pages 391-399

  Meinander, Nina Q ^a   Hahn Hägerdal, Bärbel ^a  

^a LUND INSTITUTE OF TECHNOLOGY   (Sweden)

Author keywords

plasmid stability; protein burden; recombinant Saccharomyces cerevisiae; xylitol production

Indexed keywords

BIOLOGY; BIOMEDICAL ENGINEERING; PRODUCTION; PROTEINS;

PLASMID STABILITY; XYLITOL;

BIOTECHNOLOGY;

GLUCOSE; XYLITOL;

ARTICLE; BACTERIAL GROWTH; BIOTECHNOLOGY; CULTURE MEDIUM; GENETIC RECOMBINATION; NONHUMAN; SACCHAROMYCES CEREVISIAE; STRAIN DIFFERENCE;

EID: 0343852844     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19970520)54:4<391::AID-BIT12>3.0.CO;2-J     Document Type: Article

References (35)

1. Bailey, J. E. 1993. Host-vector interactions in Escherichia coli. Adv. Biochem. Eng. 48: 29-52.
2. Barkholt, V., Jensen, A. L. 1989. Amino acid analysis: Determination of cysteine plus half-cysteine in proteins after hydrochloric acid hydrolysis with a disulfide compound as additive. Anal. Biochem. 177: 318-322.
3. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254.
4. Bugeja, V. C., Kleinman, M. J., Stanbury, P. F., Gingold, E. B. 1989. The segregation of the 2μ,-based yeast plasmid pJDB248 breaks down under conditions of slow, glucose limited growth. J. Gen. Microbiol. 135: 2891-2897.
5. Busturia, A., Lagunas, R. 1986. Catabolite inactivation of the glucose transport system in Saccharomyces cerevisiae. J. Gen. Microbiol. 132: 379-385.
6. Chen, L. F., Gong, C.-S. 1985. Fermentation of sugarcane bagasse hemicellulose hydrolysate to xylitol by hydrolysate-acclimatized yeast. J. Food. Sci. 50: 226-228.
7. Gong, C.-S., Chen, L. F., Tsao, G. T. 1981. Quantitative production of xylitol from D-xylose by a high-xylitol producing yeast mutant Candida tropicalis HXP2. Biotechnol. Lett. 3: 130-135.
8. Hallborn, J., Gorwa, M.-F., Meinander, N., Penttilä, M., Keränen, S., Hahn-Hägerdal, B. 1994. The influence of cosubstrate and aeration on xylitol formation by recombinant Saccharomyces cerevisiae expressing the XYL1 gene. Appl. Microbiol. Biotechnol. 42: 326-333.
9. Hallborn, J., Walfridsson, M., Airaksinen, U., Ojamo, H., Hahn-Hägerdal, B., Penttilä, M., Keränen, S. 1991. Xylitol production by recombinant Saccharomyces cerevisiae. Bio/Technol. 9: 1090-1095.
10. Horitsu, H., Yahashi, Y., Takamizawa, K., Kawai, K., Suzuki, T., Watanabe, N. 1992. Production of xylitol from D-xylose by Candida tropicals: Optimization of production rate. Biotechnol. Bioeng. 40: 1085-1091.
11. Koch, A. L. 1983. The protein burden of lac operon products. J. Mol. Evol. 19: 455-462.
12. Leao, C., van Uden, N. 1982. Effects of ethanol and other alkanols on the glucose transport system of Saccharomyces cerevisiae. Biotechnol. Bioeng. 24: 2601-2604.
13. Lidén, G., Walfridsson, M., Ansell, R., Anderlund, M., Adler, L., Hahn-Hägerdal, B. 1997. A glycerol-3-phosphate dehydrogenase-deficient mutant of Saccharomyces cerevisiae expressing the heterologous XYL-1 gene. Appl. Environ. Microbiol. 62: 3894-3896.
14. Lu, J., Tsai, L. B., Gong, S. C., Tsao, G. T. 1995. Effect of nitrogen sources on xylitol production from D-xylose by Candida Sp. L-102. Biotechnol. Lett. 17: 167-170.
15. Meinander, N., Hahn-Hägerdal, B., Linko, M., Linko, P., Ojamo, H. 1994. Fed-batch xylitol production with recombinant XYL1-expressing Saccharomyces cerevisiae using ethanol as a co-substrate. Appl. Microbiol. Biotechnol. 42: 334-339.
16. Meinander, N., Zacchi, G., Hahn-Hägerdal, B. 1996. A heterologous reductase affects the redox balance of recombinant Saccharomyces cerevisiae. Microbiol. 142: 165-172.
17. Mellor, J., Dobson, M. J., Roberts, N. A., Tuite, M. F., Emtage, J. S., White, S., Lowe, P. A., Patel, P. A., Kingsman, A. J., Kingsman, S. M. 1983. Efficient synthesis of enzymatically active calf chymosin in Saccharomyces cerevisiae. Gene 24: 1-14.
18. Meyrial, V., Delgenes, J. P., Moletta, R., Navarro, J. M. 1991. Xylitol production from D-xylose by Candida guillermondii: Fermentation behaviour. Biotechnol. Lett. 13: 281-286.
19. Rizzi, M., Erlemann, P., Bui-Thahn, N.-A., Dellweg, H. 1988. Xylose fermentation by yeasts. 4. Purification and kinetic studies of xylose reductase from Pichia stipitis. Appl. Microbiol. Biotechnol. 29: 148-154.
20. Roca, E., Meinander, N., Hahn-Hägerdal, B. 1996. Xylitol production by immobilized recombinant Saccharomyces cerevisiae in a continuous packed-bed bioreactor. Biotechnol. Bioeng. 51: 317-326.
21. Roseiro, J. C., Peito, M. A., Gírio, F. M., Amaral-Collaco, M. T. 1991. The effects of oxygen transfer coefficients and substrate concentration on the xylose fermentation by Debaromyces hansenii. Arch. Microbiol. 156: 484-490.
22. Sambrook, J., Fritsch, E. F., Maniatis, T. 1989. Molecular cloning: A laboratory manual. Cold Spring Harbor Laboratory. Cold Spring Harbor, New York.
23. Schiestl, R. H., Gietz, D. 1989. High efficiency transformation of intact yeast cells by single stranded nucleic acids as carrier. Curr. Genet. 16: 339-346.
24. Sherman. F., Fink, G., Hicks, J. B. 1983. Methods in yeast genetics: A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
25. Snoep, J. L., Yomano, L. P., Westerhoff, H. V., Ingram, L. O. 1995. Protein burden in Zymomonas mobilis: Negative flux and growth control due to overproduction of glycolytic enzymes. Microbiol. 141: 2329-2337.
26. Strathern, J. N., Higgins, D. R. 1991. Recovery of plasmids from yeast into Escherichia coli: Shuttle vectors, pp. 319-329. In: C. Guthrie and G. R. Fink (eds.), Guide to yeast genetics and molecular biology. Academic Press, San Diego.
27. Thestrup, H. N., Hahn-Hägerdal, B. 1995. Xylitol formation and reduction equivalent generation during anaerobic xylose conversion with glucose as cosubstrate in recombinant Saccharomyces cerevisiae expressing the XYL1 gene. Appl. Environ. Microbiol. 61: 2043-2045.
28. Thonart, P., Guerreiro, M., Foucart, M., Paquot, M. 1987. Bioconversion of xylose into xylitol by Pachysolen tannophilus. Med. Fac. Landbouww. Rijksuniv. Gent. 52: 1517-1529.
29. Walfridsson, M., Bao, X., Anderlund, M., Hahn-Hägerdal, B. Expression of different levels of enzymes from the Pichia stipitis XYL1 and XYL2 genes in Saccharomyces cerevisiae and their effects on product formation during xylose utilisation, to appear.
30. Walfridsson, M., Penttilä, M., Hahn-Hägerdal, B., Keränen, S. 1992. Recombinant Saccharomyces cerevisiae expressing xylose reductase and xylitol dehydrogenase under control of different promoters. ECB6 Sixth European Congress on Biotechnology. Firenze, Italy.
31. van Zyl, C., Prior, B. A., Kilian, S. G., Brandt, E. V. 1993. Role of D-ribose as a cometabolite in D-xylose metabolism by Saccharomyces cerevisiae. Appl. Environ. Microbiol. 59: 1487-1494.
32. Watson, N. E., Prior, B. A., du Preez, J. C., Lategan, P. M. 1984. Oxygen requirements for D-xylose fermentation to ethanol and polyols by Pachysolen tannophius. Enzyme Microb. Technol. 6: 447-450.
33. Webb, S. R., Lee, H. 1992. Characterization of xylose reductase from the yeast Pichia stiptis: Evidence for functional thiol and histidyl groups. J. Gen. Microbiol. 138: 1857-1863.
34. Verduyn, C., Postma, E., Scheffers, W. A., van Dijken, J. P. 1992. Effect of benzoic acid on metabolic fluxes in yeasts: A continuous culture study on the regulation of respiration and alcoholic fermentation. Yeast 8: 501-517.
35. Verduyn, C., Van Kleef, R., Frank, J., Schreuder, H., Van Dijken, J. P., Scheffers, W. A. 1985. Properties of the NAD(P)H-dependent xylose reductase from the xylose fermenting yeast Pichia stipitis. Biochem. J. 226: 669-677.