Subunit stoichiometry of oligomeric membrane proteins: GABA(A) receptors isolated by selective immunoprecipitation from the cell surface

Neuropharmacology

Volumn 35, Issue 9-10, 1996, Pages 1403-1411

  Kellenberger, S ^b,c   Eckenstein, S ^a   Baur, R ^a   Malherbe, P ^b   Buhr, A ^a   Sigel, E ^a  

^a UNIVERSITY OF BERN   (Switzerland)

^b F HOFFMANN LA ROCHE LTD   (Switzerland)

^c UNIVERSITY OF WASHINGTON SCHOOL OF MEDICINE   (United States)

Author keywords

GABA; GABA(A) receptor; Membrane protein; Subunit stoichiometry

Indexed keywords

4 AMINOBUTYRIC ACID; 4 AMINOBUTYRIC ACID A RECEPTOR; ANTIBODY; ANTIGEN; COMPLEMENTARY DNA; COMPLEMENTARY RNA; MEMBRANE PROTEIN; METHIONINE; MONOMER; OLIGOMER; RECEPTOR SUBUNIT; SULFUR 35;

ANIMAL CELL; ARTICLE; CONCENTRATION RESPONSE; CONTROLLED STUDY; GENE EXPRESSION; HUMAN; IMMUNOPRECIPITATION; MEMBRANE CURRENT; NONHUMAN; OOCYTE; PRIORITY JOURNAL; RAT; REGULATORY MECHANISM; STOICHIOMETRY; XENOPUS;

ANIMALS; ANTIBODIES, MONOCLONAL; AUTORADIOGRAPHY; BLOTTING, WESTERN; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HUMANS; MEMBRANE PROTEINS; METHIONINE; OOCYTES; PRECIPITIN TESTS; RECEPTORS, GABA-A; XENOPUS LAEVIS;

EID: 0030443276     PISSN: 00283908     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0028-3908(96)00034-2     Document Type: Article

References (41)

1. A receptor needs two homologous domains of the β-subunit for activation by GABA but not by pentobarbital. Nature. 366:1993;565-569.
2. Anand R., Conroy W.G., Schoepfer R., Whiting P., Lindstrom J. Neuronal nicotinic acetylcholine receptors expressed in Xenopus oocytes have a pentameric quaternary structure. J. biol. Chem. 266:1991;11192-11198.
3. 1γ2S subunits produce unique ion channels with dissimilar singlechannel conductance. J. Neurosci. 13:1993;1429-1440.
4. A receptor subunits: analysis of single-cell expression utilizing a fluorescent substrate/marker technique. J. Neurosci. 13:1993;1418-1428.
5. A receptor deduced from mutation-induced rectification. NeuroReport. 5:1993;285-288.
6. A/benzodiazepine receptor complex in granule cells of the cerebellar cortex Neuroscience. 51:1992;739-748.
7. 2/3-subunits J. biol. Chem. 266:1991;4478-4483.
8. A receptor affecting modulation of the channel by ligands of the benzodiazepine binding site. Molec. Pharmacol. 49:1996;1080-1084.
9. Cooper E., Couturier S., Ballivet M. Pentameric structure and stoichiometry of a neuronal nicotinic acetylcholine receptor. Nature. 350:1991;235-238.
10. A receptor. Int. Rev. Neurobiol. 36:1994;51-96.
11. A/benzodiazepine receptor. J. Cell Biol. 110:1990;2043-2048.
12. Fritschy J.M., Benke D., Mertens S., Oertel W.H., Bachi T., Möhler H. Five subtypes of type A γ-aminobutyric acid receptors identified in neurons by double and triple immunofluorescence staining with subunit specific antibodies. Proc. Natn. Acad. Sci. U.S.A. 89:1992;6726-6730.
13. Galzi J.L., Changeux J.P. Neurotransmitter-gated ion channels as unconventional allosteric proteins. Curr. Opin. struc. Biol. 4:1994;554-565.
14. Green W.N., Millar N.S. Ion channel assembly. TiNS. 18:1995;280-287.
15. Harlow E. and Lane D. (1988) Antibodies. A Laboratory Manual. Cold Spring Harbor Laboratory, New York.
16. A/benzodiazepine receptor from rat cerebellum. J. Neurochem. 63:1994;371-374.
17. A receptors expressed in Sf9 cells. Neurosci. Abstr. 21:1995;848.
18. A receptor confers sensitivity to benzodiazepine ligands. FEBS Lett. 293:1991;191-194.
19. A receptor function. J. Neurochem. 63:1994;1167-1170.
20. Kuhse J., Laube B., Magalei D., Betz H. Assembly of the inhibitory glycine receptor: Identification of amino acid motifs governing subunit stoichiometry. Neuron. 11:1993;1049-1056.
21. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature. 227:1970;680-685.
22. Langosch D., Thomas L., Betz H. Conserved quaternary structure of ligand-gated ion channels: the postsynaptic glycine receptor is a pentamer. Proc. Natn. Acad. Sci. U.S.A. 85:1988;7394-7398.
23. A receptor subunits expressed in Xenopus oocytes. Neuron. 1:1988;773-781.
24. A receptor channels. A. Rev. Neurosci. 17:1994;569-602.
25. A receptor determined by electron microscope image analysis. J. Neurochem. 62:1994;815-818.
26. A receptors of the α1β2 and α1β2γ2 subtypes. Molec. Pharmacol. 43:1993;801-806.
27. A receptor subunit for benzodiazepine pharmacology. Nature. 338:1989;582-585.
28. A receptors and benzodiazepine receptors in the brain shown by monoclonal antibodies. Nature. 314:1985;168-171.
29. A receptor α1 and β1 subunits. FEBS Lett. 244:1989;361-364.
30. Shimada S., Cutting G., Uhl G.R. γ-Aminobutyric acid A or C receptor? γ-Aminobutyric acid ρ1 receptor RNA induces bicuculline-, barbiturate-, and benzodiazepine-insensitive γ-aminobutyric acid responses in Xenopus oocytes. Molec. Pharmacol. 41:1992;683-687.
31. A receptor subtypes. Pharmacol. Rev. 47:1995;181-233.
32. Sigel E. Properties of single sodium channels translated by Xenopus oocytes after injection with messenger ribonucleic acid. J. Physiol. 386:1987;73-90.
33. Sigel E., Barnard E.A. A γ-aminobutyric acid/benzodiazepine receptor complex of bovine cerebral cortex: Improved purification with preservation of regulatory sites and their interactions. J. biol. Chem. 259:1984;7219-7223.
34. Sigel E., Stephenson F.A., Mamalaki C., Barnard E.A. A γ-aminobutyric acid/benzodiazepine receptor complex of bovine cerebral cortex: purification and partial characterization. J. biol. Chem. 258:1983;6965-6971.
35. A receptors oil channel function. Neuron. 5:1990;703-711.
36. A receptor channels. EMBO J. 11:1992;2017-2023.
37. A receptor α-subunit. J. biol. Chem. 269:1994;20380-20387.
38. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc. Natn. Acad. Sci. U.S.A. 76:1979;4350-4354.
39. Unwin N. Neurotransmitter action: Opening of ligandgated ion channels. Cell 72/Neuron (Suppl.). 10:1993;31-41.
40. A receptors depend upon subunit combination. Neuron. 4:1990;919-928.
41. A receptor β subunit heterogeneity: functional expression of cloned cDNAs. EMBO J. 8:1989;1665-1670.