Molecular identification of pathogenic and nonpathogenic strains of Vibrio harveyi using PCR and RAPD

Applied Microbiology and Biotechnology

Volumn 63, Issue 6, 2004, Pages 722-727

  Hernandez G ^a   Olmos, J ^a,b  

^a CENTRO DE INVESTIGACIÓN CIENTÍFICA Y DE EDUCACIÓN SUPERIOR DE ENSENADA   (Mexico)

^b Biotecnología Marina ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PRIMER DNA;

BACTERIUM; IDENTIFICATION METHOD; PATHOGENICITY; POLYMERASE CHAIN REACTION;

ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIAL VIRULENCE; CONTROLLED STUDY; GENE FUNCTION; HOST; MORTALITY; NONHUMAN; NUCLEOTIDE SEQUENCE; PATHOGENICITY; POLYMERASE CHAIN REACTION; RANDOM AMPLIFIED POLYMORPHIC DNA; REGULATOR GENE; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; SHRIMP; STRAIN DIFFERENCE; STRAIN IDENTIFICATION; VIBRIO HARVEYI;

ANIMAL DISEASES; ANIMALS; BACTERIAL PROTEINS; BACTERIAL TYPING TECHNIQUES; CRUSTACEA; DNA FINGERPRINTING; DNA, BACTERIAL; DNA, RIBOSOMAL; POLYMERASE CHAIN REACTION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; PROTEIN KINASES; RANDOM AMPLIFIED POLYMORPHIC DNA TECHNIQUE; RNA, RIBOSOMAL, 16S; TRANSCRIPTION FACTORS; VIBRIO; VIBRIO INFECTIONS; VIRULENCE; WATER MICROBIOLOGY;

BACTERIA (MICROORGANISMS); DECAPODA (CRUSTACEA); VIBRIO; VIBRIO HARVEYI;

EID: 1542359001     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-003-1451-z     Document Type: Article

References (33)

1. Aguirre-Guzmán G, Vazquez-Juárez R, Ascencio F (2001) Differences in the susceptibility of american white shrimp larval substages (Litopenaeus vannamei) to four Vibrio species. J Inv Path 78:215-219
2. Akopyanz N, Bukanov NO, Westblom TU, Kresovich S, Berg DE (1992) DNA diversity among clinical isolates of Helicobacter pylori detected by PCR-based RAPD fingerprinting. Nucleic Acids Res 20:5137-5142
3. Alabi AO, Jones DA, Latchford JW (1999) The efficacy of immersion as opposed to oral vaccination of Penaeus indicus larvae against Vibrio harveyi. Aquaculture 178:1-11
4. Alsina M, Blanch AR (1994) A set of keys for biochemical identification of environmental Vibrio species. J Appl Bacteriol 76:79-85
5. Arellano CF, Olmos SJ (2002) Thermostable α-1,4-and α-1-6-glucosidase enzymes from Bacillus sp. isolated from a marine environment. World J Microbiol Biotechnol 18:791-795
6. Bassler BL, Wright M, Showalter RE, Silverman MR (1993) Intercellular signaling in Vibrio harveyi: sequence and function of genes regulating expression of luminescence. Mol Microbiol 9:773-786
7. Bassler BL, Greenberg EP, Stevens AM (1997) Cross-species induction of luminescence in the quorum-sensing bacterium Vibrio harveyi. J Bacteriol 179:4043-4045
8. Freeman JA, Lilley BN, Bassler BL (2000) A genetic analysis of the functions of LuxN: a two-component hybrid sensor kinase that regulates quorum sensing in Vibrio harveyi. Mol Microbiol 35:139-49
9. García OM (1997) Caracterización molecular de especies marinas del género Vibrio. BSch Tesis. Universidad Autónoma de Baja California. Tijuana, Baja California, México
10. Harris LJ, Owens L (1999) Production of exotoxins by two luminous Vibrio harveyi strains known to be primary pathogens of Penaeus monodon larvae. Dis Aquat Org 38:11-22
11. Karunasagar I, Pai R, Malathi GR, Karunasagar I (1994) Mass mortality of Penaeus monodon larvae due to antibiotic-resistant Vibrio harveyi infection. Aquaculture 128:203-209
12. Kim YB, Okuda J, Matsumoto C, Takahashi N, Hashimoto S, Nishibuchi M (1999) Identification of Vibrio parahaemolyticus strains at the species level by PCR targeted to the toxR gene. J Clin Microbiol 37:1173-1177
13. Kita-Tsukamoto K, Oyaizu H, Nanba K, Simidu U (1993) Phylogenetic relationships of marine bacteria, mainly members of the family Vibrionaceae, determined on the basis of 16S rRNA sequences. Int J Syst Bacteriol 43:8-19
14. Liu PC, Lee KK, Chen SN (1996a) Pathogenicity of different isolates of Vibrio harveyi in tiger prawn, Penaeus monodon. Lett Appl Microbiol 22:413-416
15. Liu PC, Lee KK, Yii KC, Kou GH, Chen SN (1996b) Isolation of Vibrio harveyi from diseased Kuruma prawns Penaeus japonicus. Curr Microbiol 33:129-132
16. Milton D, Chalker VJ, Kirke D, Hardman A, Cámara M, Williams P (2001) The LuxM homologue VanM from Vibrio anguillarum directs the synthesis of N-(3-Hydroxyhexanoyl) homoserine lactone and n-hexanoylhomoserine lactone. J Bacteriol 183:3537-3547
17. Mukhopadhyay AK, Chakraborty S, Takeda Y, Nair GB, Berg DE (2001) Characterization of VPI pathogenicity island and CTXphi prophage in environmental strains of Vibrio cholerae. J Bacteriol 183:4737-4746
18. Olmos SJ, Paniagua MJ, Contreras FR (2000) Molecular identification of Dunaliella sp utilizing the 18S rDNA gene. Lett Appl Microbiol 30:80-84
19. Pizzutto M, Hirst RG (1995) Classification of isolates of Vibrio harveyi virulent to Penaeus monodon larvae by protein profile analysis and M13 DNA fingerprinting. Dis Aquat Org 21:61-68
20. Reichelt JL, Baumann P (1973) Taxonomy of the marine luminous bacteria. Arch Microbiol 94:283-330
21. Robertson PAW, Xu HS, Austin B (1998) An enzyme-linked immunosorbent assay (ELISA) for the detection of Vibrio harveyi in penaeid shrimp and water. J Microbiol Meth 34:31-39
22. Ruangpan L, Danayadol Y, Direkbusarakom S, Siurairatana S, Flegel TW (1999) Lethal toxicity of Vibrio harveyi to cultivated Penaeus monodon induced by a bacteriophage. Dis Aquat Org 35:195-201
23. Ruby EG, Nealson KH (1977) Seasonal changes in the species composition of luminous bacteria in the nearshore sea-water. Limmol Oceanogr 23:530-533
24. Saeed MO (1995) Association of Vibrio harveyi with mortalities in cultured marine fish in Kuwait. Aquaculture 136:21-29
25. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
26. Saulnier D, Haffner P, Goarant C, Levy P, Ansquer D (2000) Experimental infection models for shrimp vibriosis studies: a rewiew. Aquaculture 191:133-144
27. Simonet P, Grosjean MC, Misra AK, Nazaret S, Cournoyer B, Normand P (1991) Frankia genus-specific characterization by polymerase chain reaction. Appl Environ Microbiol 57:3278-86
28. Soto RS, Roque A, Lizarraga PL, Guerra FL, Gomez GB (2003) Virulence of luminous vibrios to Artemia franciscana nauplii. Dis Aquat Org 53:231-240
29. Surette MG, Miller MB, Bassler BL (1999) Quorum sensing in Escherichia coli, Salmonella typhimurium, and Vibrio harveyi: A new family of genes responsible for autoinducer production. Proc Natl Acad Sci 96:1639-1644
30. Teo JW, Suwanto A, Poh CL (2000) Novel β-lactamase genes from two environmental isolates of Vibrio harveyi. Antimicro Agents Chemother 44:1309-1314
31. Vandenberghe J, Verdonck L, Robles-Arozarena R, Rivera G, Bolland A, Balladares M, Gomez-Gil B, Calderon J, Sorgeloos P, Swings J (1999) Vibriosis associated with Litopenaeus vannamei larvae, postlarvae, broodstock, and hatchery probionts. Appl Environ Microbiol 65:2592-2597
32. West PA, Colwell RR (1984) Identification and classification of Vibrionaceae an overview. In: Colwell RR(ed), Vibrios in the environment. Wiley, New York, NY, 285-363
33. Zhang XH, Meaden PG, Austin B (2001) Duplication of hemolysin genes in a virulent isolate of Vibrio harveyi. Appl Environ Microbiol 67:3161-3167