A genetic analysis of the functions of LuxN: A two-component hybrid sensor kinase that regulates quorum sensing in Vibrio harveyi

Molecular Microbiology

Volumn 35, Issue 1, 2000, Pages 139-149

  Freeman, Jeremy A ^a   Lilley, Brendan N ^a   Bassler, Bonnie L ^a  

^a PRINCETON UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ASPARTIC ACID; HISTIDINE; PHOSPHATASE; PROTEIN KINASE; REGULATOR PROTEIN;

ARTICLE; CELL DENSITY; CONTROLLED STUDY; DELETION MUTANT; ENZYME ACTIVITY; GENETIC ANALYSIS; NONHUMAN; PHENOTYPE; POINT MUTATION; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN PHOSPHORYLATION; TRANSCRIPTION REGULATION; VIBRIO HARVEYI;

BACTERIAL PROTEINS; BASE SEQUENCE; DNA, RECOMBINANT; MUTAGENESIS, SITE-DIRECTED; PHOSPHORIC MONOESTER HYDROLASES; PROTEIN KINASES; RECOMBINANT PROTEINS; TRANSCRIPTION FACTORS; VIBRIO;

EID: 0033985890     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.2000.01684.x     Document Type: Article

References (32)

1. Appleby, J.L., Parkinson, S., and Bourret, R.B. (1996) Signal transduction via the multi-step phosphorelay: not necessarily a road less traveled. Cell 86: 845-848.
2. Arico, B., Miller, J.F., Roy, C., Stibitz, S., Monack, D., Falkow, S., et al. (1989) Sequences required for expression of Bordetella pertussis virulence factors share homology with prokaryotic signal transduction proteins. Proc Natl Acad Sci USA 86: 6671-6675.
3. Bassler, B.L. (1999a) A multichannel two-component signaling relay controls quorum sensing in Vibrio harveyi. In Cell-Cell Signaling in Bacteria. Dunny, G.M., and Winans, S.C. (eds). Washington, DC: American Society for Microbiology Press, pp. 259-273.
4. Bassler, B.L. (1999b) How bacteria talk to each other: regulation of gene expression by quorum sensing. Curr Opin Microbiol (in press).
5. Bassler, B.L., Wright, M., Showalter, R.E., and Silverman, M.R. (1993) Intercellular signalling in Vibrio harveyi: sequence and function of genes regulating expression of luminescence. Mol Microbiol 9: 773-786.
6. Bassler, B.L., Wright, M., and Silverman, M.R. (1994a) Multiple signalling systems controlling expression of luminescence in Vibrio harveyi: sequence and function of genes encoding a second sensory pathway. Mol Microbiol 13: 273-286.
7. Bassler, B.L., Wright, M., and Silverman, M.R. (1994b) Sequence and function of luxO, a negative regulator of luminescence in Vibrio harveyi. Mol Microbiol 12: 403-412.
8. Bassler, B.L., Greenberg, E.P., and Stevens, A.M. (1997) Cross-species induction of luminescence in the quorum-sensing bacterium Vibrio harveyi. J Bacteriol 179: 4043-4045.
9. Beringer, J.E., Beynon, J.L., Buchanan-Wollaston, A.V., and Johnston, A.W.B. (1978) Transfer of the drug resistance transposon Tn5 to Rhizobium. Nature 276: 633-634.
10. Brissette, R.E., Tsung, K., and Inouye, M. (1991) Suppression of a mutation in OmpR at the putative phosphorylation center by a mutant EnvZ protein in Escherichia coli. J Bacteriol 173: 601-608.
11. Burbulys, D.K., Trach, K.A., and Hoch, J.A. (1991) The initiation of sporulation in Bacillus subtilis is controlled by a multicomponent phosphorelay. Cell 64: 545-552.
12. Cao, J., and Meighen, E.A. (1989) Purification and structural identification of an autoinducer for the luminescence system of Vibrio harveyi. J Biol Chem 264: 21670-21676.
13. Ditta, G., Stanfield, S., Corbin, D., and Helinski, D. (1980) Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti. Proc Natl Acad Sci USA 77: 7347-7351.
14. Fellay, R., Frey, J., and Krisch, H. (1987) Interposon mutagenesis of soil and water bacteria: a family of DNA fragments designed for in vitro insertional mutagenesis of Gram-negative bacteria. Gene 52: 147-154.
15. Freeman, J.A., and Bassler, B.L. (1999a) Sequence and function of LuxU: a two-component phosphorelay protein that regulates quorum sensing in Vibrio harveyi. J Bacteriol 181: 899-906.
16. Freeman, J.A., and Bassler, B.L. (1999b) A genetic analysis of the function of LuxO, a two-component response regulator involved in quorum sensing in Vibrio harveyi. Mol Microbiol 31: 665-677.
17. Friedman, A., Long, S.R., Brown, S.E., Buikema, W.J., and Ausubel, F. (1982) Construction of a broad host range cosmid cloning vector and its use in the genetic analysis of Rhizobium mutants. Gene 18: 289-296.
18. Georgellis, D., Lynch, A.S., and Lin, E.C.C. (1997) In vitro phosphorylation study of the Arc two-component signal transduction system of Escherichia coli. J Bacteriol 179: 5429-5435.
19. Georgellis, D., Kwon, O., De Wulf, P., and Lin, E.C.C. (1998) Signal decay through a reverse phosphorelay in the Arc two-component signal transduction system. J Biol Chem 273: 32864-32869.
20. Greenberg, E.P., Hastings, J.W., and Ulitzer, S. (1979) Induction of luciferase synthesis in Beneckea harveyi by other marine bacteria. Arch Microbiol 120: 87-91.
21. Hsing, W., and Silhavy, T.J. (1997) Function of conserved histidine-243 in phosphatase activity of EnvZ, the sensor for porin osmoregulation in Escherichia coli. J Bacteriol 179: 3729-3735.
22. Iuchi, S., Matsuda, T., Fujiwara, T., and Lin, E.C.C. (1990) The arcB gene of Escherichia coli encodes a sensor-regulator protein for anaerobic repression of the arc modulon. Mol Microbiol 4: 715-727.
23. Manoil, C.M., and Beckwith, J. (1985) TnphoA: a transposon probe for protein export signals. Proc Natl Acad Sci USA 82: 8129-8133.
24. Michael, S.F. (1994) Mutagenesis by incorporation of a phosphorylated oligo during PCR amplification. Biotechniques 16: 410-412.
25. Parkinson, J., and Kofoid, E.C. (1992) Communication modules in bacterial signaling proteins. Annu Rev Genet 26: 71-112.
26. Posas, F., Wurgler-Murphy, S.M., Maeda, T., Witten, E.A., Thai, T.C., and Saito, H. (1996) Yeast HOG1 MAP kinase cascade is regulated by a multistep phosphorelay mechanism in the SLN1-YPD1-SSK1 'two-component' osmosensor. Cell 86: 865-875.
27. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
28. Stock, J.B., Ninfa, A.J., and Stock, A.M. (1989) Protein phosphorylation and regulation of adaptive responses in bacteria. Microbiol Rev 53: 450-490.
29. Surette, M.G., Miller, M.B., and Bassler, B.L. (1999) Quorum sensing in Escherichia coli, Salmonella typhimurium, and Vibrio harveyi: a new family of genes responsible for auto-inducer production. Proc Natl Acad Sci USA 96: 1639-1644.
30. Tokishita, S., Kojima, A., and Mizuno, T. (1992) Transmembrane signal transduction and osmoregulation in Escherichia coli: functional importance of the transmembrane regions of membrane-localized protein kinase, EnvZ. J Biochem 111: 707-713.
31. Uhl, M.A., and Miller, J.F. (1994) Autophosphorylation and phosphotransfer in the Bordetella pertussis BvgAS signal transduction cascade. Proc Natl Acad Sci USA 91: 1163-1167.
32. Uhl, M.A., and Miller, J.F. (1996) Central role of the BvgS receiver as a phosphorylated intermediate in a complex two-component phosphorelay. J Biol Chem 271: 33176-33180.