Cell-free synthesis of polypeptides lacking an amino-terminal methionine by using a dicistroviral intergenic internal ribosome entry site

Journal of Biochemistry

Volumn 136, Issue 5, 2004, Pages 601-606

  Shibuya, Norihiro ^a   Nishiyama, Takashi ^a   Nakashima, Nobuhiko ^a  

^a NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES   (Japan)

Author keywords

Cell free protein synthesis; Dicistroviridae; Internal ribosome entry site; Translation initiation; Wheat germ extract

Indexed keywords

ION; LUCIFERASE; METHIONINE; NUCLEOTIDE DERIVATIVE; POLYPEPTIDE; TRANSFER RNA;

AMINO TERMINAL SEQUENCE; ARTICLE; CELL FREE SYSTEM; CONCENTRATION (PARAMETERS); GENE DELETION; GENE SEQUENCE; INTERNAL RIBOSOME ENTRY SITE; PROTEIN SYNTHESIS; START CODON; WHEAT GERM;

CELL-FREE SYSTEM; MAGNESIUM; METHIONINE; PEPTIDES; POTASSIUM; PROTEIN BIOSYNTHESIS; RIBOSOMES; RNA VIRUSES;

DICISTROVIRIDAE; TRITICUM AESTIVUM;

EID: 14944346224     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/jb/mvh167     Document Type: Article

References (32)

1. Kozak, M. (1999) Initiation of translation in prokaryotes and eukaryotes. Gene 234, 187-208
2. Hershey, J.W.B. and Merrick, W.C. (2000) Translational Control of Gene Expression (Sonenberg, N., Hershey, J.W.B. and Mathews, M.B., eds.) pp. 33-88, Cold Spring Harbor Laboratory Press, New York
3. Endo, S., Yamamoto, Y., Sugawara, T., Nishimura, O., and Fujino, M. (2001) The additional methionine residue at the N-terminus of bacterially expressed human interleukin-2 affects the interaction between the N- and C- termini. Biochemistry 40, 914-919
4. 2 and N-terminal variant: kinetic parameters and response to inhibitors. J. Biochem. 113, 734-737
5. Mine, S., Ueda, T., Hashimoto, Y., and Imoto, T. (1997) Improvement of the refolding yield and solubility of hen egg-white lysozyme by altering the Met residue attached to its N-terminus to Ser. Protein Eng. 10, 1333-1338
6. Nishimura, O., Suenaga, M., Ohmae, H., Tsuji, S., and Fujino, M. (1998) An efficient chemical method for removing N-terminal extra methionine from recombinant methionylated human growth hormone. Chem. Commun. 1135-1136
7. Mayer, C., Köhrer, C., Kenny, E., Prusko, C., and RajBhandary, U.L. (2003) Anticodon sequence mutants of Escherichia coli initiator tRNA: effects of overproduction of aminoacyl-tRNA synthetases, methionyl-tRNA formyltransferase, and initiation factor 2 on activity in initiation. Biochemistry 42, 4787-4799
8. Liao Y., Jeng, J., Wang, C., Wang, S., and Chang, S. (2004) Removal of N-terminal methionine from recombinant proteins by engineered E. coli methionine aminopeptidase. Protein Sci. 13, 1802-1810
9. de Felipe, P., Hughes, L.E., Ryan, M.D., and Brown, J.D. (2003) Co-translational, intraribosomal cleavage of polypeptides by the foot-and-mouth disease virus 2A peptide. J. Biol. Chem. 278, 11441-11448
10. Wood, D.W., Wu, W., Belfort, G., Derbyshire, V., and Belfort, M (1999) A genetic system yields self-cleaving inteins for bioseparations. Nat. Biotech. 17, 889-892
11. Catanzariti, A.M., Soboleva, T.A., Jans, D.A., Board, P.G., and Baker, R.T. (2004) An efficient system for high-level expression and easy purification of authentic recombinant proteins. Protein Sci. 13, 1331-1339
12. Mayo, M.A. (2002) A summary of taxonomic changes recently approved by ICTV. Arch. Virol. 147, 1655-1656
13. Sasaki, J. and Nakashima, N. (1999) Translation initiation at the CUU codon is mediated by the internal ribosome entry site of an insect picorna-like virus in vitro. J. Virol. 73, 1219-1226
14. Domier, L.L., McCoppin, N.K., and D'Arcy, C.J. (2000) Sequence requirements for translation initiation of Rhopalosiphum padi virus ORF2. Virology 268, 264-271
15. Wilson, J.E., Powell, M.J., Hoover, S.E., and Sarnow, P. (2000) Naturally occurring dicistronic cricket paralysis virus RNA is regulated by two internal ribosome entry sites. Mol. Cell. Biol. 20, 4990-4999
16. Hatakeyama, Y., Shibuya, N., Nishiyama, T., and Nakashima, N. (2004) Structural variant of the intergenic internal ribosome entry site elements in dicistroviruses and computational search for their counterparts. RNA 10, 779-786
17. Hellen, C.U.T. and Sarnow, P. (2001) Internal ribosome entry sites in eukaryotic mRNA molecules. Genes Dev. 15, 1593-1612
18. Kanamori, Y. and Nakashima, N. (2001) A tertiary structure model of the internal ribosome entry site (IRES) for methionine-independent initiation of translation. RNA 7, 266-274
19. Sasaki, J. and Nakashima, N. (2000) Methionine-independent initiation of translation in the capsid protein of an insect RNA virus. Proc. Natl Acad. Sci. USA 97, 1512-1515
20. Wilson, J.E., Pestova, T.V., Hellen, C.U.T., and Sarnow, P. (2000) Initiation of protein synthesis from the A site of the ribosome. Cell 102, 511-520
21. Jan, E. and Sarnow, P. (2002) Factorless ribosome assembly on the internal ribosome entry site of cricket paralysis virus. J. Mol. Biol. 324, 889-902
22. Nishiyama, T., Yamamoto, H., Shibuya, N., Hatakeyama, Y., Hachimori, A., Uchiumi, T., and Nakashima, N. (2003) Structural elements in the internal ribosome entry site of Plautia stali intestine virus responsible for binding with ribosomes. Nucleic Acids Res. 31, 2434-2442
23. Pestova, T.V. and Hellen, C.U.T. (2003) Translation elongation after assembly of ribosomes on the cricket paralysis virus internal ribosome entry site without initiation factors or initiator tRNA. Genes Dev. 17, 181-186
24. Jan, E., Kinzy, T.G., and Sarnow, P. (2003) Divergent tRNA-like element supports initiation, elongation, and termination of protein biosynthesis. Proc. Natl Acad. Sci. USA 100, 15410-15415
25. Shibuya, N., Nishiyama, T., Kanamori, Y., Saito, H., and Nakashima, N. (2003) Conditional rather than absolute requirements of the capsid coding sequence for initiation of methionine-independent translation in Plautia stali intestine virus. J. Virol. 77, 12002-12010
26. Borman, A.M., Bailly, J.L., Girard, M., and Kean, K.M. (1995) Picornavirus internal ribosome entry segments: comparison of translation efficiency and the requirements for optimal internal initiation of translation in vitro. Nucleic Acids Res. 23, 3656-3663
27. Jackson, R.J. (1991) Potassium salts influence the fidelity of mRNA translation initiation in rabbit reticulocyte lysates: unique features of encephalomyocarditis virus RNA translation. Biochim. Biophys. Acta 26, 345-358
28. Madin, K., Sawasaki, T., Ogasawara, T., and Endo, Y. (2000) A highly efficient and robust cell-free protein synthesis system prepared from wheat embryos: plants apparently contain a suicide system directed at ribosomes. Proc. Natl Acad. Sci. USA 97, 559-564
29. Pöyry, T.A.A., Kaminski, A., and Jackson, R.J. (2004) What determines whether mammalian ribosomes resume scanning after translation of a short upstream open reading frame? Genes Dev. 18, 62-75
30. Moore, N.F., Reavy, B., Pullin, J.S.K., and Plus, N. (1981) The proteins expressed by different isolates of Drosophila C virus. Virology 112, 411-416
31. Nemoto, N., Miyamoto-Sato, E., Husimi, Y., and Yanagawa, H. (1997) In vitro virus: bonding of mRNA bearing puromycin at the 3′-terminal end to the C-terminal end of its encoded protein on the ribosome in vitro. FEBS Lett. 414, 405-408
32. Xu, H., Petersen, E.I., Petersen, S.B., and El-Gewely, M.R. (1999) Random mutagenesis libraries: optimization and simplification by PCR. Biotechniques 27, 1102-1108