Conserved N-terminal negative charges in the Tim17 subunit of the TIM23 translocase play a critical role in the import of preproteins into mitochondria

Journal of Biological Chemistry

Volumn 280, Issue 9, 2005, Pages 7777-7785

  Meier, Stephan ^a   Neupert, Walter ^a   Herrmann, Johannes M ^a  

^a UNIVERSITY OF MUNICH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; BIOCHEMISTRY; BIOLOGICAL MEMBRANES; GENETIC ENGINEERING; HYDROPHILICITY;

ALANINE; MITOCHONDRIAL MEMBRANES; PREPROTEINS;

PROTEINS;

ALANINE; AMINO ACID; ASPARTIC ACID; CARRIER PROTEIN; PROTEIN PRECURSOR; PROTEIN SUBUNIT; PROTEIN TIM 17; PROTEIN TIM 23; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; CHANNEL GATING; CHEMICAL ANALYSIS; ENZYME MECHANISM; GENETIC ANALYSIS; HUMAN; IN VITRO STUDY; MITOCHONDRIAL MEMBRANE; MITOCHONDRION; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYLOGENY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FUNCTION;

AMINO ACID MOTIFS; AMINO ACID SEQUENCE; ASPARTIC ACID; CHROMATOGRAPHY, GEL; CROSS-LINKING REAGENTS; DNA; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENES, DOMINANT; GLUCOSE; GLYCEROL; IMMUNOPRECIPITATION; MEMBRANE POTENTIALS; MEMBRANE TRANSPORT PROTEINS; MITOCHONDRIA; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PROTEIN CONFORMATION; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; PROTEIN TRANSPORT; RECOMBINANT PROTEINS; REPRESSOR PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; TIME FACTORS;

EID: 14844334925     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M412158200     Document Type: Article

References (37)

1. Sickmann, A., Reinders, J., Wagner, Y., Joppich, C., Zahedi, R., Meyer, H. E., Schonfisch, B., Perschil, I., Chacinska, A., Guiard, B., Rehling, P., Pfanner, N., and Meisinger, C. (2003) Proc. Natl. Acad. Sci. U. S. A. 100, 13207-13212
2. Kumar, A., Agarwal, S., Heyman, J. A., Matson, S., Heidtman, M., Piccirillo, S., Umansky, L., Drawid, A., Jansen, R., Liu, Y., Cheung, K. H., Miller, P., Gerstein, M., Roeder, G. S., and Snyder, M. (2002) Genes Dev. 16, 707-719
3. Wiedemann, N., Frazier, A. E., and Pfanner, N. (2004) J. Biol. Chem. 279, 14473-14476
4. Hoogenraad, N. J., Ward, L. A., and Ryan, M. T. (2002) Biochim. Biophys. Acta 1592, 97-105
5. Neupert, W., and Brunner, M. (2002) Nat. Rev. Mol. Cell. Biol. 3, 555-565
6. Koehler, C. M. (2000) FEBS Lett. 476, 27-31
7. von Heijne, G. (1986) EMBO J. 5, 1335-1342
8. Komiya, T., Rospert, S., Koehler, C., Looser, R., Schatz, G., and Mihara, K. (1998) EMBO J. 17, 3886-3898
9. Mayer, A., Neupert, W., and Lill, R. (1995) Cell 80, 127-137
10. Geissler, A., Chacinska, A., Truscott, K. N., Wiedemann, N., Brandner, K., Sickmann, A., Meyer, H. E., Meisinger, C., Pfanner, N., and Rehling, P. (2002) Cell 111, 507-518
11. Yamamoto, H., Esaki, M., Kanamori, T., Tamura, Y., Nishikawa, S., and Endo, T. (2002) Cell 111, 519-528
12. Mokranjac, D., Paschen, S. A., Kozany, C., Prokisch, H., Hoppins, S. C., Nargang, F. E., Neupert, W., and Hell, K. (2003) EMBO J. 22, 816-825
13. Bauer, M. F., Sirrenberg, C., Neupert, W., and Brunner, M. (1996) Cell 87, 33-41
14. Lohret, T. A., Jensen, R. E., and Kinnally, K. W. (1997) J. Cell Biol. 137, 377-386
15. Truscott, K N., Kovermann, P., Geissler, A., Merlin, A., Meijer, M., Driessen, A. J., Rassow, J., Pfanner, N., and Wagner, R. (2001) Nat. Struct. Biol. 8, 1074-1082
16. Pfanner, N., and Chacinska, A. (2002) Biochim. Biophys. Acta 1592, 15-24
17. Kozany, C., Mokranjac, D., Sichting, M., Neupert, W., and Hell, K. (2004) Nat. Struct. Mol. Biol. 11, 234-241
18. Frazier, A. E., Dudek, J., Guiard, B., Voos, W., Li, Y., Lind, M., Meisinger, C., Geissler, A., Sickmann, A., Meyer, H. E., Bilanchone, V., Cumsky, M. G., Truscott, K. N., Pfanner, N., and Rehling, P. (2004) Nat. Struct. Mol. Biol. 11, 226-233
19. Truscott, K. N., Voos, W., Frazier, A. E., Lind, M., Li, Y., Geissler, A., Dudek, J., Muller, H., Sickmann, A., Meyer, H. E., Meisinger, C., Guiard, B., Rehling, P., and Pfanner, N. (2003) J. Cell Biol. 163, 707-713
20. Mokranjac, D., Sichting, M., Neupert, W., and Hell, K. (2003) EMBO J. 22, 4945-4956
21. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) in Molecular Cloning, 2nd Ed. (Nolan, C., ed), CSH Laboratory Press, Cold Spring Harbor, NY
22. Vernet, T., Dignard, D., and Thomas, D. Y. (1987) Gene (Amst.) 52, 225-233
23. Sikorski, R. S., and Hieter, P. (1989) Genetics 122, 19-27
24. Brachmann, C. B., Davies, A., Cost, G. J., Caputo, E., Li, J., Hieter, P., and Boeke, J. D. (1998) Yeast 14, 115-132
25. Boeke, J. D., LaCroute, F., and Fink, G. R. (1984) Mol. Gen. Genet. 197, 345-346
26. Herrmann, J. M., Fölsch, H., Neupert, W., and Stuart, R. A. (1994) in Cell Biology: A Laboratory Handbook (Celis, J. E., ed) Vol. 1, pp. 538-544, Academic Press, San Diego, CA
27. Herrmann, J. M., Neupert, W., and Stuart, R. A. (1997) EMBO J. 16, 2217-2226
28. Herrmann, J. M., Westermann, B., and Neupert, W. (2001) Methods Cell Biol. 65, 217-230
29. Gärtner, F., Voos, W., Querol, A., Miller, B. R., Craig, E. A., Cumsky, M. G., and Pfanner, N. (1995) J. Biol. Chem. 270, 3788-3795
30. Szyrach, G., Ott, M., Bonnefoy, N., Neupert, W., and Herrmann, J. M. (2003) EMBO J. 22, 6448-6457
31. Glaser, S. M., Miller, B. R., and Cumsky, M. G. (1990) Mol. Cell. Biol. 10, 1873-1881
32. Martin, J., Mahlke, K., and Pfanner, N. (1991) J. Biol. Chem. 266, 18051-18057
33. Pfanner, N., and Neupert, W. (1985) EMBO J. 4, 2819-2825
34. Schneider, H.-C., Berthold, J., Bauer, M. F., Dietmeier, K., Guiard, B., Brunner, M., and Neupert, W. (1994) Nature 371, 768-774
35. Donzeau, M., Kaldi, K., Adam, A., Paschen, S., Wanner, G., Guiard, B., Bauer, M. F., Neupert, W., and Brunner, M. (2000) Cell 101, 401-412
36. Field, L. S., Furukawa, Y., O'Halloran, T. V., and Culotta, V. C. (2003) J. Biol. Chem. 278, 28052-28059
37. Iwata, S., Saynovits, M., Link, T. A., and Michel, H. (1996) Structure 4, 567-5791