Tim50 is a subunit of the TIM23 complex that links protein translocation across the outer and inner mitochondrial membranes

Cell

Volumn 111, Issue 4, 2002, Pages 519-528

  Yamamoto, Hayashi ^a   Esaki, Masatoshi ^a   Kanamori, Takashi ^b   Tamura, Yasushi ^a   Nishikawa, Shuh ichi ^a   Endo, Toshiya ^a  

^a NAGOYA UNIVERSITY   (Japan)

^b NIPPON MEDICAL SCHOOL   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CARRIER PROTEIN; MITOCHONDRIAL PROTEIN; PROTEIN ANTIBODY; PROTEIN TIM23; PROTEIN TIM50; UNCLASSIFIED DRUG;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CROSS LINKING; MEMBRANE TRANSPORT; MITOCHONDRIAL MEMBRANE; NONHUMAN; OUTER MEMBRANE; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN FUNCTION; PROTEIN PROTEIN INTERACTION; PROTEIN TRANSPORT; TRANSPORT KINETICS; YEAST;

EID: 0037111988     PISSN: 00928674     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0092-8674(02)01053-X     Document Type: Article

References (35)

1. Abe Y., Odaka M., Inagaki F., Lax I., Schlessinger J., Kohda D. Disulfide bond structure of human epidermal growth factor receptor. J. Biol. Chem. 273:1998;11150-11157.
2. Ahting U., Thieffry M., Engelhardt H., Hegerl R., Neupert W., Nussberger S. Tom40, the pore-forming component of the protein-conducting TOM channel in the outer membrane of mitochondria. J. Cell Biol. 153:2001;1151-1160.
3. o-ATPase. the novel subunit e is identical to Tim11 FEBS Lett. 411:1997;195-200.
4. Bauer M.F., Sirrenberg C., Neupert W., Brunner M. Role of Tim23 as voltage sensor and presequence receptor in protein import into mitochondria. Cell. 87:1996;33-41.
5. Bauer M.F., Hoffman S., Neupert W., Brunner M. Protein translocation into mitochondria. the role of TIM complexes Trends Cell Biol. 10:2000;25-31.
6. Berthold J., Bauer M.F., Schneider H.-C., Klaus C., Dietmeier K., Neupert W., Brunner M. The MIM complex mediates preprotein translocation across the mitochondrial inner membrane and couples it to the mt-Hsp70εATP driving system. Cell. 81:1995;1085-1093.
7. 2 branches from the general mitochondrial import pathway at the preprotein translocase of the inner membrane. J. Biol. Chem. 272:1997;30439-30446.
8. Claros M.G., Vincens P. Computational method to predict mitochondrially imported proteins and their targeting sequences. Eur. J. Biochem. 241:1996;779-786.
9. Court D.A., Nargang F., Steiner H., Hodges R.S., Neupert W., Lill R. Role of the intermembrane-space domain of the preprotein receptor Tom22 in protein import into mitochondria. Mol. Cell. Biol. 16:1996;4035-4042.
10. Dekker P.J., Martin F., Maarse A.C., Bomer U., Muller H., Guiard B., Pfanner N. The Tim core complex defines the number of mitochondrial translocation contact sites and can hold arrested preproteins in the absence of matrix Hsp70-Tim44. EMBO J. 16:1997;5408-5419.
11. Diehn M., Eisen M.B., Botstein D., Brown P.O. Large-scale identification of secreted and membrane-associated gene products using DNA microarrays. Nat. Genet. 25:2000;58-62.
12. Donzeau M., Káldi K., Adam A., Paschen S., Wanner G., Guiard B., Bauer M.F., Neupert W., Brunner M. Tim23 links the inner and outer mitochondrial membranes. Cell. 101:2000;401-412.
13. Endo T., Kohda D. Functions of outer membrane receptors in mitochondrial protein import. Biochim. Biophys. Acta. 1592:2002;3-14.
14. 2 import pathway. Proc. Natl. Acad. Sci. USA. 96:1999;11770-11775.
15. Hill K., Model K., Ryan M.T., Dietmeier K., Martin F., Wagner R., Pfanner N. Tom40 forms the hydrophilic channel of the mitochondrial import pore for preproteins. Nature. 395:1998;516-521.
16. Horst M., Hilfiker-Rothenfluh S., Oppliger W., Schatz G. Dynamic interaction of the protein translocation systems in the inner and outer membranes of yeast mitochondria. EMBO J. 14:1995;2293-2297.
17. James P., Halladay J., Craig E.A. Genomic libraries and a host strain designed for highly efficient two-hybrid selection in yeast. Genetics. 144:1996;1425-1436.
18. Kanamori T., Nishikawa S., Shin I., Schultz G., Endo T. Probing the environment along the protein import pathways in yeast mitochondria by site-specific photocrosslinking. Proc. Natl. Acad. Sci. USA. 94:1997;485-490.
19. Kanamori T., Nishikawa S., Nakai M., Shin I., Schultz P.G., Endo T. Uncoupling of transfer of the presequence and unfolding of the mature domain in precursor translocation across the mitochondrial outer membrane. Proc. Natl. Acad. Sci. USA. 96:1999;3634-3639.
20. Koehler C.M., Merchant S., Schatz G. How membrane proteins travel across the mitochondrial intermembrane space. Trends Biochem. Sci. 24:1999;428-432.
21. Komiya T., Rospert S., Koehler C., Looser R., Schatz G., Mihara K. Interaction of mitochondrial targeting signals with acidic receptor domains along the protein import pathway. evidence for the 'acid chain' hypothesis EMBO J. 17:1998;3886-3898.
22. Mayer A., Neupert W., Lill R. Mitochondrial protein import. reversible binding of the presequence at the trans side of the outer membrane drives partial translocation and unfolding Cell. 80:1995;127-137.
23. Moczko M., Bömer U., Kübrich M., Zufall N., Hönlinger A., Pfanner N. The intermembrane space domain of mitochondrial Tom22 functions as a trans binding site for preproteins with N-terminal targeting sequences. Mol. Cell. Biol. 17:1997;6574-6584.
24. Neupert W. Protein import into mitochondria. Annu. Rev. Biochem. 66:1997;863-917.
25. Paschen S.A., Rothbauer U., Káldi K., Bauer M.F., Neupert W., Brunner M. The role of the TIM8-13 complex in the import of Tim23 into mitochondria. EMBO J. 19:2000;6392-6400.
26. Pfanner N., Rassow J., van der Klei I., Neupert W. A dynamic model of the mitochondrial protein import machinery. Cell. 68:1992;999-1002.
27. Pfanner N., Geissler A. Versatility of the mitochondrial protein import machinery. Nat. Rev. Mol. Cell Biol. 2:2001;339-349.
28. Rapaport D., Neupert W., Lill R. Mitochondrial protein import; Tom40 plays a major role in targeting and translocation of preproteins by forming a specific binding site for the presequence. J. Biol. Chem. 272:1997;18725-18731.
29. Rose M.D., Winston F., Hieter P. Methods in Yeast Genetics. A Laboratory Course Manual:1990;Cold Spring Harbor, NY, Cold Spring Harbor Laboratory Press.
30. Schleyer M., Neupert W. Transport of proteins into mitochondria. translocation intermediates spanning contact sites between the outer and inner membranes Cell. 43:1985;339-350.
31. Siniossoglou S., Santos-Rosa H., Rappsilber J., Mann M., Hurt E. A novel complex of membrane proteins required for formation of a spherical nucleus. EMBO J. 17:1998;6449-6464.
32. Siniossoglou S., Hurt E.C., Pelham H.R.B. Psr1p/Psr2p, two plasma membrane phosphatases with an essential DXDX(T/V) motif required for sodium stress response in yeast. J. Biol. Chem. 275:2000;19352-19360.
33. Sirrenberg C., Endres M., Becker K., Bauer M.F., Walther E., Neupert W., Brunner M. Functional cooperation and stoichiometry of protein translocases of the outer and inner membranes of mitochondria. J. Biol. Chem. 272:1997;29963-29966.
34. Tokatlidis K., Junne T., Moes S., Schatz G., Glick B.S., Kronidou N. Translocation arrest of an intramitochondrial sorting signal next to Tim11 at the inner-membrane import site. Nature. 384:1996;585-588.
35. Truscott K.N., Kovemann P., Geissler A., Merlin A., Meijer M., Driessen A.J.M., Rassow J., Pfanner N., Wagner R. A presequence- and voltage-sensitive channel of the mitochondrial preprotein translocase formed by Tim23. Nat. Struct. Biol. 8:2001;1074-1082.