Rho-dependent and -indenpendent activation mechanisms of ezrin/radixin/moesin proteins: An essential role for polyphosphoinositides in vivo

Journal of Cell Science

Volumn 115, Issue 12, 2002, Pages 2569-2580

  Yonemura, Shigenobu ^a   Matsui, Takeshi ^a   Tsukita, Shoichiro ^a   Tsukita, Sachiko ^a  

^a KYOTO UNIVERSITY   (Japan)

Author keywords

ERM protein; Microvilli; Neomycin; Ptdlns(4,5)P2; Rho

Indexed keywords

ACTIN; AMINOGLYCOSIDE ANTIBIOTIC AGENT; EZRIN; MOESIN; MONOCLONAL ANTIBODY; NEOMYCIN; PHOSPHATIDYLINOSITOL 4,5 BISPHOSPHATE; POLYPHOSPHOINOSITIDE; PROTEIN KINASE INHIBITOR; RADIXIN; RECOMBINANT EPIDERMAL GROWTH FACTOR; RHO FACTOR; STAUROSPORINE; THREONINE; TRANSFERASE; ACTIN BINDING PROTEIN; BOTULINUM TOXIN; CYTOSKELETON PROTEIN; DIAGNOSTIC AGENT; EPIDERMAL GROWTH FACTOR; EXOENZYME C3, CLOSTRIDIUM BOTULINUM; MEMBRANE PROTEIN; NICOTINAMIDE ADENINE DINUCLEOTIDE ADENOSINE DIPHOSPHATE RIBOSYLTRANSFERASE; PHOSPHATIDYLINOSITOL; PHOSPHOPROTEIN; PLASMA PROTEIN; RHO GUANINE NUCLEOTIDE BINDING PROTEIN;

ACTIN FILAMENT; ANIMAL CELL; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL LINE; CELL MEMBRANE; CELL STIMULATION; CELL TYPE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CYTOPLASM; HUMAN; HUMAN CELL; IN VIVO STUDY; KIDNEY CELL; MICROINJECTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN CROSS LINKING; PROTEIN DEPHOSPHORYLATION; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN PHOSPHORYLATION; PROTEIN TRANSPORT; ANIMAL; CELL STRAIN 3T3; CHO CELL; CYTOSKELETON; DRUG EFFECT; ELECTRON MICROSCOPY; EUKARYOTIC CELL; FLUORESCENT ANTIBODY TECHNIQUE; HAMSTER; HELA CELL; METABOLISM; MICROVILLUS; MOUSE; PHYSIOLOGY; PROTEIN TERTIARY STRUCTURE; ULTRASTRUCTURE;

3T3 CELLS; ADP RIBOSE TRANSFERASES; ANIMALS; BLOOD PROTEINS; BOTULINUM TOXINS; CELL MEMBRANE; CHO CELLS; CRICETINAE; CYTOSKELETAL PROTEINS; CYTOSKELETON; EPIDERMAL GROWTH FACTOR; EUKARYOTIC CELLS; FLUORESCENT ANTIBODY TECHNIQUE; HELA CELLS; HUMANS; MEMBRANE PROTEINS; MICE; MICROFILAMENT PROTEINS; MICROSCOPY, ELECTRON; MICROVILLI; PHOSPHATIDYLINOSITOLS; PHOSPHOPROTEINS; PROTEIN STRUCTURE, TERTIARY; PROTEIN TRANSPORT; RHO GTP-BINDING PROTEINS;

EID: 0037096169     PISSN: 00219533     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (67)

1. Ezrin contains cytoskeleton and membrane binding domains accounting for its proposed role as a membrane-cytoskeletal linker
2. 2-terminal domain of ezrin correlates with its altered cellular distribution
3. Ezrin oligomers are major cytoskeletal components of placental microvilli: A proposal for their involvement in cortical morphogenesis
4. Rapid phosphorylation and reorganization of ezrin and spectrin accompany morphological changes induced in A-431 cells by epidermal growth factor
5. Regulation of cortical structure by the ezrin-radixin-moesin protein family
6. Soluble ezrin purified from placenta exists as stable monomers and elongated dimers with masked C-terminal ezrin-radixin-moesin association domains
7. ras and phospholipases: A comparative study
8. Dephosphorylation of ezrin as an early event in renal microvillar breakdown and anoxic injury
9. Rapid induction of morphological changes in human carcinoma cells A-431 by epidermal growth factor
10. The FERM domain: A unique module involved in the linkage of cytoplasmic proteins to membrane
11. Two G-proteins act in series to control stimulus-secretion coupling in mast cells: Use of neomycin to distinguish between G-proteins controlling polyphosphoinositide phosphodiesterase and exocytosis
12. Ezrin is an effector of hepatocyte growth factor-mediated migration and morphogenesis in epithelial cells
13. Normal development of mice and unimpaired cell adhesion/cell motility/actin-based cytoskeleton without compensatory up-regulation of ezrin or radixin in moesin gene knockout
14. Moesin, like ezrin, colocalizes with actin in the cortical cytoskeleton in cultured cells, but its expression is more variable
15. Binding of neomycin to phosphatidylinositol 4,5-bisphosphate (PIP2)
16. Ezrin self-association involves binding of an N-terminal domain to a normally masked C-terminal domain that includes the F-actin binding site
17. Structural basis of the membrane-targeting and unmasking mechanism of the radxin FERM domain
18. Thrombin receptor ligation and activated Rac uncap actin filament barbed ends through phosphoinositide synthesis in permeabilized human platelets
19. Immunofluorescence detection of ezrin/radixin/moesin (ERM) proteins with their carboxyl-terminal threonine phosphorylated in cultured cells and tissues: Application of a novel fixation protocol using trichloroacetic a fixative
20. Association of ezrin with intercellular adhesion molecule-1 and -2 (ICAM-1 and ICAM-2)
21. ICAM-2 redistributed by ezrin as a target for killer cells
22. Molecular dissection of radixin: Distinct and interdependent functions of the amino- and carboxyl-terminal domains
23. Regulation mechanism of ERM protein/plasma membrane association: Possible involvement of phosphatidylinositol turnover and rho-dependent signaling pathway
24. Phosphatidylinositol 4-phosphate 5-kinase a is a downstream effector of the small G protein ARF6 in membrane ruffle formation
25. Replacement of threonine 558, a critical site of phosphorylation of moesin in vivo with aspartate activates F-actin binding of moesin
26. Cloning of cDNAs encoding two isoforms of 68-kDa type I phosphatidylinositol-4-phosphate 5-kinase
27. Gentamicin-induced loss of basolateral surface area of rat proximal convoluted tubules
28. ERM(ezrin/radixin/moesin)-based molecular mechanism of microvillar breakdown at an early stage of apoptosis
29. Rho regulates association of both the ERM family and vinculin with the plasma membrane in MDCK cells
30. Effects of noise and ototoxic drugs at the cellular level in the cochlea: A review
31. Inhibition of neural phospholipase D activity by aminoglycoside antibiotics
32. ERM proteins in cell adhesion and membrane dynamics
33. 2-terminal domain inhibits the cell extension activity of the COOH-terminal domain
34. Rho-kinase phosphorylates COOH-terminal threonines of ezrin/radixin/moesin(ERM) proteins and regulates their head-to-tail association
35. Activation of ERM proteins in vivo by Rho involves phosphatidylinositol 4-phosphate 5-kinase and not ROCK kinases
36. NHE-RF, a regulatory cofactor for Na(+)-H+ exchange, is a common interactor for merlin and ERM (MERM) proteins
37. Phosphorylation of threonine 558 in the carboxyl-terminal actin-binding domain of moesin by thrombin activation of human platelets
38. Regulation of F-actin binding to platelet moesin in vitro by both phosphorylation of threonine 558 and polyphosphatidylinositeds
39. Substrate for botulinum ADP-ribosyltransferase, Gb, has an amino acid sequence homologous to a putative Rho gene product
40. Structural properties of lipid-binding sites in cytoskeletal proteins
41. Identification of a phosphatidylinositol-4, 5-bisphosphate-binding domain in the N-terminal region of ezrin
42. Phosphorylation of moesin by rho-associated kinase (Rho-kinase) plays a crucial role in the formation of microvilli-like structures
43. Structure of ERM protein moesin reveals the FERM domain fold masked by an extended actin binding tail domain
44. Moesin, ezrin, and p205 are actin-binding proteins associated with neutrophil plasma membranes
45. Identification of EBP-50: A PDZ-containing phosphoprotein that associates with members of the ezrin-radixin-moesin family
46. Identification of two strains of MDCK cells which resembles separate nephron tubule segments
47. The small GTP-binding protein rho regulates the assembly of focal adhesions and stress fibers in response to growth factors
48. Radixin, a barbed end-capping actin-modulating protein, is concentrated at the cleavage furrow during cytokinesis
49. Purification of polyphosphoinositides by chromatography on immobilized neomycin
50. Moesin interacts with the cytoplasmic region of intercellular adhesion molecule-3 and is redistributed to the uropod of T lymphocytes during cell polarization
51. Rho-A-dependent phosphorylation and relocalization of ERM proteins into apical membrane/actin protrusions in fibroblasts
52. C-terminal threonine phosphorylation activates ERM proteins to link the cell's cortical lipid bilayer to the cytoskeleton
53. Sperm-induced currents at fertilization in sea urchin eggs injected with EGTA and neomycin
54. Perturbation of cell adhesion and microvilli formation by antisense oligonucleotides to ERM family members
55. Rho, Rac and Cdc43 GTPases regulate the organization of the actin cytoskeleton
56. ERM family members as molecular linkers between the cell surface glycoprotein CD44 and actin-based cytoskeletons
57. Cortical actin organization: Lessons from ERM (ezrin/radixin/moesin) proteins
58. Nephrotoxicity of aminoglycoside antibiotics
59. Ezrin has a COOH-terminal actin-binding site that is conserved in the ezrin protein family
60. Calcium sensitization of smooth muscle mediated by a Rho-associated protein kinase in hypertension
61. The ezrin protein family: Membrane-cytoskeleton interactions and disease associations
62. Concentration of an integral membrane protein, CD43 (leukosialin, sialophorin), in the cleavage furrow through the interaction of its cytoplasmic domain with actin-based cytoskeletons
63. Ezrin/radixin/moesin (ERM) proteins bind to a positively charged amino acid cluster in the juxta-membrane cytoplasmic domain of CD44, CD43, and ICAM-2
64. Direct involvement of ezrin/radixin/moesin (ERM)-binding membrane proteins in the organization of microvilli in collaboration with activated ERM proteins
65. + exchanger, NHE3, requires an associated regulatory protein
66. Characterization of aminoglycoside-lipid interactions and development of a refined model for ototoxicity testing
67. Experiments on ototoxic effects of antibiotics