The production and localization of GTP-bound ran in mitotic mammalian tissue culture cells

Cell Cycle

Volumn 3, Issue 8, 2004, Pages 991-993

  Li, Hoi Yeung ^a   Zheng, Yixian ^a  

^a HOWARD HUGHES MEDICAL INSTITUTE   (United States)

Author keywords

Cell cycle; Mitosis; Mitotic spindle; Ran; RanBP1; RanGAP1; RCC1

Indexed keywords

GUANOSINE TRIPHOSPHATASE; GUANOSINE TRIPHOSPHATE; NEF PROTEIN; RAN PROTEIN; RANBPI PROTEIN; RANGAP 1 PROTEIN; RCC1 PROTEIN; UNCLASSIFIED DRUG;

ANIMAL CELL; CELL CYCLE; HUMAN; HUMAN CELL; HYDROLYSIS; INTERPHASE; MAMMAL CELL; MITOSIS; MITOSIS SPINDLE; NONHUMAN; NUCLEOCYTOPLASMIC TRANSPORT; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN SYNTHESIS; REVIEW; TISSUE CULTURE;

EID: 13944279881     PISSN: 15384101     EISSN: 15514005     Source Type: Journal    
DOI: 10.4161/cc.3.8.1059     Document Type: Review

References (20)

1. Quimby BB, Dasso M. The small GTPase Ran: interpreting the signs. Curr Opin Cell Biol 2003; 15:1-7.
2. Weis K. Nucleocytoplasmic transport: cargo trafficking across the border. Curr Opin Cell Biol 2002; 14:328-35.
3. Gruss OJ, Carazo-Salas RE, Schatz CA, Guarguaglini G, Kast J, Wilm M, Le Bot N, Vernos I, Karsenti E, Mattaj IW. Ran induces spindle assembly by reversing the inhibitory effect of importin α on TPX2 activity. Cell 2001; 104:83-92.
4. Nachury MV, Maresca TJ, Salmon WC, Waterman-Storer CM, Heald R, Weis K. Importin β is a mitotic target of the small GTPase Ran in spindle assembly. Cell 2001; 104:95-106.
5. Wiese C, Wilde A, Moore MS, Adam SA, Merdes A, Zheng Y. Role of importin-β in coupling Ran to downstream targets in microtubule assembly. Science 2001; 291:653-56.
6. Schatz CA, Santarella R, Hoenger A, Karsenti E, Mattaj IW, Gruss OJ, Carazo-Salas RE. Importin α-regulated nucleation of microtubules by TPX2. EMBO J 2003; 22:2060-70.
7. Ems-McClung SC, Zheng Y, Walczak CE. Importin α/β and Ran-GTP regulate XCTK2 microtubule binding through a bipartite nuclear localization signal. Mol Biol Cell 2004; 15:46-57.
8. Seino H, Hisamoto N, Uzawa S, Sekiguchi T, Nishimoto T. DNA-binding domain of RCC1 protein is not essential for coupling mitosis with DNA replication. J Cell Sci 1992; 102:393-400.
9. Carazo-Salas RE, Guarguaglini G, Gruss OJ, Segref A, Karsenti E, Mattaj IW. Generation of GTP-bound Ran by RCC1 is required for chromatin-induced mitotic spindle formation. Nature 1999; 400:178-81.
10. Nemergut ME, Mizzen CA, Stukenberg T, Allis CD, Macara IG. Chromatin docking and exchange activity enhancement of RCC1 by histones H2A and H2B. Science 2001; 292:1540-43.
11. Li H, Wirtz D, Zheng Y. A mechanism of coupling RCC1 mobility to RanGTP production on the chromatin in vivo. J Cell Biol 2003; 160:635-44.
12. Kalab P, Weis K, Heald R. Visualization of a Ran-GTP gradient in interphase and mitotic Xenopus egg extracts. Science 2002; 295:2452-6.
13. Gorlich D, Seewald MJ, Ribbeck K. Characterization of Ran-driven cargo transport and the RanGTPase system by kinetic measurements and computer simulation. EMBO J 2003; 22:1088-100.
14. Li HY, Zheng Y. Phosphorylation of RCC1 in mitosis is essential for producing a high RanGTP concentration on chromosomes and for spindle assembly in mammalian cells. Genes Dev 2004; 18:512-27.
15. Bilbao-Cortes D, Hetzer M, Langst G, Becker PB, Mattaj IW. Ran binds to chromatin by two distinct mechanisms. Curr Biol 2002; 12:1151-56.
16. Bischoff FR, Krebber H, Smirnova E, Dong W, Ponstingl H. Co-activation of RanGTPase and inhibition of GTP dissociation by Ran-GTP binding protein RanBP1. EMBO J 1995; 14:705-15.
17. Floer M, Blobel G, Rexach M. Disassembly of RanGTP-karyopherin b complex, an intermediate in nuclear protein import. J Biol Chem 1997; 272:19538-46.
18. Lounsbury KM, Macara IG. Ran-binding protein 1 (RanBP1) forms a ternary complex with Ran and karyopherin beta and reduces Ran GTPase-activating protein (RanGAP) inhibition by karyopherin beta. J Biol Chem 1997; 272:551-5.
19. Guarguaglini G, Renzi L, D'Ottavio F, Di Fiore B, Casenghi M, Cundari E, Lavia P. Regulated Ran-binding protein 1 activity is required for organization and function of the mitotic spindle in mammalian cells in vivo. Cell Growth Differ. 2000; 11:455-65.
20. Joseph J, Tan SH, Karpova TS, McNally JG, Dasso M. SUMO-1 targets RanGAP1 to kinetochores and mitotic spindles. J Cell Biol 2002; 156:595-602.