Myosin-XVa is required for tip localization of whirlin and differential elongation of hair-cell stereocilia

Nature Cell Biology

Volumn 7, Issue 2, 2005, Pages 148-156

  Belyantseva, Inna A ^a   Boger, Erich T ^a,b   Naz, Sadaf ^a   Frolenkov, Gregory I ^a   Sellers, James R ^c   Ahmed, Zubair M ^a   Griffith, Andrew J ^a   Friedman, Thomas B ^a  

^a NATIONAL INSTITUTE ON DEAFNESS AND OTHER COMMUNICATION DISORDERS   (United States)

^b University of Maryland   (United States)

^c NATIONAL HEART LUNG AND BLOOD INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIN; GREEN FLUORESCENT PROTEIN; MOLECULAR MOTOR; MYOSIN; MYOSIN 15A; PDZ PROTEIN; UNCLASSIFIED DRUG; WHIRLIN;

ANIMAL CELL; ANIMAL TISSUE; APICAL MEMBRANE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL ELONGATION; CONTROLLED STUDY; GENETIC TRANSFECTION; HAIR CELL; HAIR GROWTH; HEAD MOVEMENT; IN VIVO STUDY; INNER EAR; MICROVILLUS; MORPHOGENESIS; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; RAT; SENSE ORGAN; SOUND DETECTION; STEREOCILIUM; STRAIN DIFFERENCE; WILD TYPE;

ACTINS; ANIMALS; CELLS, CULTURED; CERCOPITHECUS AETHIOPS; CILIA; COS CELLS; DEAFNESS; GREEN FLUORESCENT PROTEINS; HAIR CELLS, INNER; MEMBRANE PROTEINS; MICE; MICE, MUTANT STRAINS; MODELS, BIOLOGICAL; MOLECULAR MOTOR PROTEINS; MYOSINS;

ANIMALIA;

EID: 13944260197     PISSN: 14657392     EISSN: None     Source Type: Journal    
DOI: 10.1038/ncb1219     Document Type: Article

References (30)

1. DeRosier, D.J. & Tilney, L.G. F-actin bundles are derivatives of microvilli: What does this tell us about how bundles might form? J. Cell Biol. 148, 1-6 (2000).
2. Tilney, L.G. & Tilney, M.S. Functional organization of the cytoskeleton. Hear. Res. 22, 55-77 (1986).
3. Tilney, L.G., Tilney, M.S. & DeRosier, D.J. Actin filaments, stereocilia, and hair cells: how cells count and measure. Annu. Rev. Cell Biol. 8, 257-274 (1992).
4. Probst, F.J. et al. Correction of deafness in shaker-2 mice by an unconventional myosin in a BAC transgene. Science 280, 1444-1447 (1998).
5. Holme, R.H., Kiernan, B.W., Brown, S.D. & Steel, K.P. Elongation of hair cell stereocilia is defective in the mouse mutant whirler. J. Comp. Neurol. 450, 94-102 (2002).
6. Mburu, P. et al. Defects in whirlin, a PDZ domain molecule involved in stereocilia elongation, cause deafness in the whirler mouse and families with DFNB31. Nature Genet. 34, 421-428 (2003).
7. Edds, K.T. Dynamic aspects of filopodial formation by reorganization of microfilaments. J. Cell Biol. 73, 479-491 (1977).
8. Tilney, L.G., Bonder, E.M. & DeRosier, D.J. Actin filaments elongate from their membrane-associated ends. J. Cell Biol. 90, 485-494 (1981).
9. Mallavarapu A. & Mitchison, T. Regulated actin cytoskeleton assembly at filopodium tips controls their extension and retraction J. Cell Biol. 146, 1097-1106 (1999).
10. Schneider, M.E., Belyantseva, I.A., Azevedo, R.B. & Kachar, B. Rapid renewal of auditory hair bundles. Nature 418, 837-838 (2002).
11. Bartles, J.R. Parallel actin bundles and their multiple actin-bundling proteins. Curr. Opin. Cell Biol. 12, 72-78 (2000).
12. Frolenkov, G.I., Belyantseva, I.A., Friedman, T.B. & Griffith, A.J. Genetic insights into the morphogenesis of inner ear hair cells. Nature Rev. Genet. 5, 489-498 (2004).
13. Gorelik, J. et al. Dynamic assembly of surface structures in living cells. Proc. Natl Acad. Sci. USA 100, 5819-5822 (2003).
14. Tilney, L.G., Tilney, M.S., Saunders, J.S. & DeRosier, D.J. Actin filaments, stereocilia, and hair cells of the bird cochlea. III. The development and differentiation of hair cells and stereocilia. Dev. Biol. 116, 100-118 (1986).
15. Kaltenbach, J.A., Falzarano, P.R. & Simpson, T.H. Postnatal development of the hamster cochlea. II. Growth and differentiation of stereocilia bundles. J. Comp. Neurol. 350, 187-198 (1994).
16. Zine, A. & Romand, R. Development of the auditory receptors of the rat: a SEM study. Brain Res. 721, 49-58 (1996).
17. Tilney, L.G., Tilney, M.S. & Cotanche, D.A. Actin filaments, stereocilia, and hair cells of the bird cochlea. V. How the staircase pattern of stereociliary lengths is generated. J. Cell Biol. 106, 355-365 (1988).
18. Wang, A. et al. Association of unconventional myosin MYO15 mutations with human nonsyndromic deafness DFNB3. Science 280, 1447-1451 (1998).
19. Belyantseva, I.A., Boger, E.T. & Friedman, T.B. Myosin XVa localizes to the tips of inner ear sensory cell stereocilia and is essential for staircase formation of the hair bundle. Proc. Natl Acad. Sci. USA 100, 13958-13963 (2003).
20. Anderson, D.W. et al. The motor and tail regions of myosin XV are critical for normal structure and function of auditory and vestibular hair cells. Hum. Mol. Genet. 9, 1729-1738 (2000).
21. Tokuo, H. & Ikebe, M. Myosin X transports Mena/VASP to the tip of filopodia. Biochem. Biophys. Res. Commun. 319, 214-220 (2004).
22. Rzadzinska, A.K., Schneider, M.E., Davies, C., Riordan, G.P. & Kachar, B. An actin molecular treadmill and myosins maintain stereocilia functional architecture and self-renewal. J. Cell Biol. 164, 887-897 (2004).
23. Hasson, T. et al. Unconventional myosins in inner-ear sensory epithelia. J. Cell Biol. 137, 1287-1307 (1997).
24. Selve, N. & Wegner, A. Rate of treadmilling of actin filaments in vitro. J. Mol. Biol. 187, 627-631 (1986).
25. Shimada, T., Sasaki, N., Ohkura, R. & Sutoh, K. Alanine scanning mutagenesis of the switch I region in the ATPase site of Dictyostelium discoideum myosin II. Biochemistry 36, 14037-14043(1997).
26. Kambara, T. et al. Functional significance of the conserved residues in the flexible hinge region of the myosin motor domain. J. Biol. Chem. 274, 16400-16406 (1999).
27. Liang, Y. et al. Characterization of the human and mouse unconventional myosin XV genes responsible for hereditary deafness DFNB3 and shaker 2. Genomics 61, 243-258 (1999).
28. Sheng, M. & Sala, C. PDZ domains and the organization of supramolecular complexes. Annu. Rev. Neurosci. 24, 1-29 (2001).
29. Berg, J.S. & Cheney, R.E. Myosin-X is an unconventional myosin that undergoes intra-filopodial motility. Nature Cell Biol. 4, 246-250 (2002).
30. Sekerkova, G. et al. Espins are multifunctional actin cytoskeletal regulatory proteins in the microvilli of chemosensory and mechanosensory cells. J. Neurosci. 24, 5445-5456 (2004).