Reduced DNA gap repair in aging rat neuronal extracts and its restoration by DNA polymerase β and DNA-ligase

Journal of Neurochemistry

Volumn 92, Issue 4, 2005, Pages 818-823

  Krishna, T Hari ^a   Mahipal, S ^a   Sudhakar, A ^b   Sugimoto, H ^b   Kalluri, Raghu ^b   Rao, K Subba ^a  

^a UNIVERSITY OF HYDERABAD   (India)

^b BETH ISRAEL DEACONESS MEDICAL CENTER   (United States)

Author keywords

Aging brain; DNA gap repair; DNA ligase; DNA polymerase

Indexed keywords

DNA DIRECTED DNA POLYMERASE BETA; POLYDEOXYRIBONUCLEOTIDE SYNTHASE; RECOMBINANT ENZYME;

AGING; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CELL FUNCTION; CONTROLLED STUDY; ENZYME DEFICIENCY; EXCISION REPAIR; NERVE CELL; NONHUMAN; PRIORITY JOURNAL; RAT;

AGING; ANIMALS; CEREBRAL CORTEX; DNA LIGASES; DNA POLYMERASE BETA; DNA REPAIR; NEURONS; OLIGODEOXYRIBONUCLEOTIDES; RATS; RATS, WISTAR;

EID: 13644271465     PISSN: 00223042     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1471-4159.2004.02923.x     Document Type: Article

References (21)

1. Cabelof D. C., Raffoul J. J., Yanamadala S., Ganir C., Guo Z. and Heydari A. R. (2002) Attenuation of DNA-polymerase beta dependent base excision repair and increased DMS-induced mutagenecity in aged mice. Mutat. Res. 500, 135-145.
2. Chagovetz A. M., Sweasy J. B. and Preston B. D. (1997) Increased activity and fidelity of DNA polymerase β on single nucleotide gapped DNA. J. Biol. Chem. 272, 27 501-27 504.
3. Dianov G., Price A. and Lindhal T. (1992) Generation of single nucleotide repair patches following excision of uracil residues from DNA. Mol. Cell. Biol. 12, 1605-1612.
4. Dianov G. L., Prasad R., Wilson S. H. and Bohr V. A. (1999) Role of DNA polymerase β in the excision step of long patch mammalian base excision repair. J. Biol. Chem. 274, 13 741-13 743.
5. Fortini P., Pascucci B., Parlanthi E., Sobol R. W., Wilson S. H. and Dogliotti E. (1998) Different DNA polymerases are involved in the short and long patch base excision repair in mammalian cells. Biochemistry 37, 3575-3580.
6. Hoeijmakers J. H. (2001) Genome maintenance mechanisms for preventing cancer. Nature 411, 366-374.
7. Idriss H. T., Al-Assar O. and Wilson S. H. (2002) DNA polymerase β. Int. J. Biochem. Cell Biol. 34, 321-324.
8. Memisoglu A. and Samson L. (2000) Base excision repair in yeast and mammals. Mutat. Res. 451, 39-51.
9. Podlutsky A. J., Dianova I. I., Podust V. N., Bohr V. A. and Dianov G. L. (2001) Human DNA polymerase β initiates DNA synthesis during long-patch repair of reduced AP sites in DNA. EMBO J. 20, 1477-1482.
10. Prapurna D. R. and Rao K. S. (1997) DNA polymerase δ and ε in developing and aging rat brain. Int. J. Dev. Neurosci. 15, 67-73.
11. Prasad R., Beard W. A. and Wilson S. H. (1994) Studies of gapped DNA substrate binding by mammalian DNA polymerase β: dependence on 5′-phosphate group. J. Biol. Chem. 269, 18 096-18 101.
12. Raji N. S., Krishna T. H. and Rao K. S. (2002) DNA-polymerase α,β,δ and ε activities in isolated neuronal and astroglial cell fractions from developing and aging rat cerebral cortex. Int. J. Dev Neurosci. 20, 491-496.
13. Rao K. S. (2003) DNA repair and brain aging: the importance of base excision repair and DNA polymerase β. Proc. Indian Natl Acad. Sci. B69, 141-156.
14. Rao K. S., Annapuma V. V., Raji N. S. and Harikrishna T. (2000) Loss of base excision repair and its restoration by DNA polymerase β in aging rat neurons. Brain Res. Mol. Brain Res. 85, 251-259.
15. Singhal R. K. and Wilson S. H. (1993) Short gap-filling synthesis by DNA polymerase β is processive. J. Biol. Chem. 268, 15 906-15 911.
16. Tomkinson A. E. and Mackey Z. B. (1998) Structure and function of mammalian DNA ligases. Mutat. Res. 407, 1-9.
17. Usha Rani B., Indrapal Singh N., Ray A. and Rao K. S. (1983) Procedure for isolation of neuron and astrocyte enriched fractions from chick brain of different ages. J. Neurosci. Res. 10, 101-105.
18. Waser J., Hubscher U., Kuenzle C. C. and Spadari S. (1979) DNA polymerase β from neurons is repair enzyme. Eur. J. Biochem. 97, 361-368.
19. Wilson D. M. III and Thompson L. H. (1997) Life without DNA repair. Proc. Natl Acad. Sci. USA 94, 12 754-12 757.
20. Wilson S. H. (1998) Mammalian base excision repair and DNA polymerase beta. Mutat. Res. 407, 203-215.
21. Wood R. D. and Shivji M. K. (1997) Which DNA polymerases are used for DNA-repair in eukaryotes? Carcinogenesis 18, 605-610