Metabolic engineering of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) composition in recombinant Salmonella enterica serovar Typhimurium

Biotechnology and Bioengineering

Volumn 76, Issue 2, 2001, Pages 108-114

  Ilana, Aldor ^a   Keasling, J D ^a  

^a JOHANNES GUTENBERG UNIVERSITY   (Germany)

Author keywords

Biodegradable plastic; PHA; PHBV; Polyhydroxyalkanoate; Propionyl CoA synthetase; PrpE

Indexed keywords

BIOPOLYMERS; COPOLYMERS; METABOLISM; ORGANIC COMPOUNDS; SUBSTRATES;

BIOPLASTICS;

BIOTECHNOLOGY;

ARABINOSE; COPOLYMER; GENE PRODUCT; PLASTIC; POLY(3 HYDROXYBUTYRIC ACID); POLYHYDROXYALKANOIC ACID; PROPIONYL COENZYME A; SYNTHETASE; VALERIC ACID DERIVATIVE;

ACINETOBACTER; ARTICLE; BIODEGRADATION; BIOENGINEERING; CHEMICAL COMPOSITION; CONCENTRATION RESPONSE; CONTROLLED STUDY; ENZYME SUBSTRATE; NONHUMAN; PROMOTER REGION; SALMONELLA ENTERICA;

ACINETOBACTER SP.; SALMONELLA ENTERICA; SALMONELLA ENTERICA SUBSP. ENTERICA SEROVAR TYPHIMURIUM; TYPHIMURIUM;

EID: 0034850801     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.1150     Document Type: Article

References (53)

1. End of biopol
2. Polyhydroxyalkanoates, biopolyesters from renewable resources: Physiological and engineering aspects
3. Polymer synthesis by microorganisms: Technology and economics
4. High-level production of poly(3-hydroxybutyrateco-3-hydroxyvalerate) by fed-batch culture of recombinant Escherichia coli
5. Properties and applications of polyhydroxyalkanoates
6. Production of heteropolymeric polyhydroxyalkanoate in Escherichia coli from a single carbon source
7. Polyhydroxyalkanoate production in recombinant Escherichia coli
8. Isolation and characterization of homogeneous acetate kinase from Salmonella typhimurium and Escherichia coli
9. Characterization of 13 kDa granule-associated protein in Aeromonas caviae and biosynthesis of polyhydroxyalkanoates with altered molar composition by recombinant bacteria
10. Overexpression and purification of the soluble polyhydroxyalkanote synthase from Alcaligenes eutrophus: Evidence for a required posttranslational modification for catalytic activity
11. BAD promoter
12. Propionate catabolism in Salmonella typhimurium LT2: Two divergently transcribed units comprise the prp locus at 8.5 centisomes, prpR encodes a member of the sigma-54 family of activators, and the prpBCDE genes constitute an operon
13. Salmonella typhimurium LT2 catabolizes propionate via the 2-methylcitric acid cycle
14. The prpE gene of Salmonella typhimurium LT2 encodes propionyl-CoA synthetase
15. Artificial promoters for metabolic optimization
16. The sequence of spacers between the consensus sequences modulates the strength of prokaryotic promoters
17. Metabolic engineering of Alcaligenes eutrophus through the transformation of cloned phbCAB genes for the investigation of the regulatory mechanism of polyhydroxyalkanoate synthesis
18. Biosynthesis of polyhydroxyalkanoates (PHA) by recombinant Ralstonia eutropha and effects of PHA synthase activity on in vivo PHA biosynthesis
19. Discontinuity of homology of Escherichia coli and Salmonella typhimurium DNA in the lac region
20. Identification and heterologous expression of the polyhydroxyalkanoate biosynthesis genes from Alcaligenes sp. SH-69
21. E. coli moves into the plastic age
22. Enhanced biosynthesis of P(3HB-3HV) and P(3HB-4HB) by amplification of the cloned PHB biosynthesis genes in Alcatigenes eutrophus
23. Carbon-13 nuclear magnetic resonance study of metabolism of propionate by Escherichia coli
24. Good intentions
25. Overproduction and dissection of proteins by the expression cassette polymerase chain reaction
26. Chain termination in polyhydroxyalkanoate synthesis: Involvement of exogenous hydroxy-compounds as chain transfer agents
27. Metabolic engineering of poly(3-hydroxyalkanoates): From DNA to plastic
28. A series of wide-host-range low-copy-number vectors that allow direct screening for recombinants
29. Culture medium for enterobacteria
30. Enzyme induction as an all-or-none phenomenon
31. prpR, ntrA, and ihf functions are required for expression of the prpBCDE operon, encoding enzymes that catabolize propionate in Salmonella enterica serovar Typhimurium LT2
32. Production of polyhydroxyalkanoates, a family of biodegradable plastics and elastomers, in bacteria and plants
33. The function of ackA and pta genes is necessary for poly(3-hydroxybutyrate-co-3-hydroxyvalerate) synthesis in recombinant pha+ Escherichia coli
34. + Escherichia coli
35. Gas chromatographic determination of poly-β-hydroxybutyric acid in microbial biomass after hydrochloric acid propanolysis
36. Phosphate concentration regulates transcription of the Acinetobacter polyhydroxyalkanoic acid biosynthetic genes
37. Identification of a 13-kDa protein associated with the polyhydroxyalkanoic acid granules from Acinetobacter spp.
38. Gene expression from plasmids containing the araBAD promoter at subsaturating inducer concentrations represents mixed populations
39. PHA synthase activity controls the molecular weight and polydispersity of polyhydroxybutyrate in vivo
40. Production of poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) in a recombinant Escherichia coli strain
41. Multiple β-ketothiolases mediate poly(β-hydroxyalkanoate) copolymer synthesis in Ralstonia eutropha
42. Metabolic engineering of Arabidopsis and Brassica for poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer production
43. Physiology and molecular genetics of poly(β-hydroxyalkanoic acid) synthesis in Alcaligenes eutrophus
44. Propionate oxidation in Escherichia coli: Evidence for operation of a methylcitrate cycle in bacteria
45. Non-genetic population heterogeneity studied by in situ polymerase chain reaction
46. Transformation of restriction-deficient Salmonella typhimurium LT2
47. Studies of regulation of expression of the propionate (prpBCDE) operon provide insights into how Salmonella typhimurium LT2 integrates its 1,2-propanediol and propionate catabolic pathways
48. PHA production, from bacteria to plants
49. Application of a propionyl coenzyme A synthetase for poly(3-hydroxy propionate-co-3-hydroxybutyrate) accumulation in recombinant Escherichia coli
50. Effect of acetic acid on poly-(3hydroxybutyrate-co-3-hydroxyvalerate) synthesis in recombinant Escherichia coli
51. Synthesis of poly-(3-hydroxybutyrateco-3-hydroxyvalerate) by recombinant Escherichia coli
52. New insight into the role of the PhaP phasin of Ralstonia eutropha in promoting synthesis of polyhydroxybutyrate