Direct visualization of lipid phase segregation in single lipid bilayers with coherent anti-stokes Raman scattering microscopy

ChemPhysChem

Volumn 6, Issue 1, 2005, Pages 77-79

  Potma, Eric O ^a   Xie, X Sunney ^a  

^a HARVARD UNIVERSITY   (United States)

Author keywords

CARS (coherent anti Stokes Raman scattering); Fluorescence; Phospholipids; Vesicles; Vibrational spectroscopy

Indexed keywords

BINARY MIXTURES; COHERENT SCATTERING; FLUORESCENCE; LIPID BILAYERS; PHOSPHOLIPIDS; RAMAN SPECTROSCOPY; VIBRATIONAL SPECTROSCOPY;

COHERENT ANTI STOKES RAMAN SCATTERING; COHERENT ANTI-STOKES RAMAN SCATTERING MICROSCOPY; DIRECT VISUALIZATION; GIANT UNILAMELLAR VESICLES; GIANT VESICLES; SINGLE-LIPID BILAYERS; VESICLES; VIBRATIONAL MODES;

RAMAN SCATTERING;

LIPID;

ARTICLE; CHEMICAL MODIFICATION; IMAGING; LIPID BILAYER; MICROSCOPY; RAMAN SPECTROMETRY; VIBRATION;

EID: 13444253811     PISSN: 14394235     EISSN: None     Source Type: Journal    
DOI: 10.1002/cphc.200400390     Document Type: Article

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24. A calibration curve can be calculated based on the known quadratic CARS signal dependence on the concentration, under the assumption that the vibrational spectra of the lipids remain invariant for different mixture ratios. In addition, for an accurate determination of the concentration, knowledge is required on the real and imaginary parts of the CARS spectrum. This information can be obtained from CARS spectral measurements.