A subcellular prefractionation protocol for minute amounts of mammalian cell cultures and tissue

Proteomics

Volumn 5, Issue 1, 2005, Pages 35-45

  Guillemin, Isabelle ^a   Becker, Michael ^a   Ociepka, Kornelia ^a   Friauf, Eckhard ^a   Nothwang, Hans Gerd ^a  

^a Rheinland Pfälzische Technische Universität Kaiserslautern Landau   (Germany)

Author keywords

Benzyldimethyl n hexodecylammonium chloride sodium dodecyl sulfate; Mass spectrometry; Subcellular fractionation; Two dimensional gel electrophoresis

Indexed keywords

AMMONIUM CHLORIDE; BENZYLDIMETHYL N HEXADECYLAMMONIUM CHLORIDE; DODECYL SULFATE SODIUM; HISTONE; MEMBRANE PROTEIN; PROTEIN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL CULTURE; CELL FRACTIONATION; CELL MEMBRANE; CELL NUCLEUS; CELLULAR DISTRIBUTION; CYTOPLASM; EMBRYO; FEMALE; HUMAN; HUMAN CELL; IMMUNOBLOTTING; MALE; MAMMAL CELL; MASS SPECTROMETRY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN LOCALIZATION; PROTEOMICS; RAT; SAMPLE SIZE; TISSUE CULTURE; TWO DIMENSIONAL GEL ELECTROPHORESIS;

ANIMALS; BRAIN CHEMISTRY; CELL LINE; CELL MEMBRANE; CERCOPITHECUS AETHIOPS; CYTOPLASM; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FEMALE; HUMANS; IMMUNOBLOTTING; MALE; MASS SPECTROMETRY; MEMBRANE PROTEINS; NUCLEAR PROTEINS; ORGANELLES; PROTEOME; RATS; RATS, SPRAGUE-DAWLEY; SUBCELLULAR FRACTIONS;

ANIMALIA; MAMMALIA;

EID: 13244273504     PISSN: 16159853     EISSN: None     Source Type: Journal    
DOI: 10.1002/pmic.200400892     Document Type: Article

References (34)

1. Aebersold, R., Mann, M., Nature 2003, 422, 198-207.
2. Mootha, V. K., Bunkenborg, J., Olsen, J. V., Hjerrild, M. et al., Cell 2003, 115, 629-640.
3. Miklos, G. L. G., Maleszka, R., Proteomics 2001, 1, 30-41.
4. Hanash, S., Nature 2003, 422, 226-232.
5. Fountoulakis, M., Juranville, J. F., Anal. Biochem. 2003, 313, 267-282.
6. Ghaemmaghami, S., Huh, W., Bower, K., Howson, R. W. et al., Nature 2003, 425, 737-741.
7. Badock, V., Steinhusen, U., Bommert, K., Otto, A., Electrophoresis 2001, 22, 2856-2864.
8. Herbert, B., Righetti, P. G., Electrophoresis 2000, 21, 3639-3648.
9. Davidsson, P., Puchades, M., Blennow, K., Electrophoresis 1999, 20, 431-437.
10. Molloy, M. P., Herbert, B. R., Walsh, B. J., Tyler, M. I. et al., Electrophoresis 1998, 19, 837-844.
11. Washburn, M. P., Wolters, D., Yates, J. R., Nat. Biotechnol. 2001, 19, 242-247.
12. Wu, C. C., MacCoss, M. J., Howell, K. E., Yates, J. R., Nat. Biotechnol. 2003, 21, 532-538.
13. Gevaert, K., Goethals, M., Martens, L., Van Damme, J. et al., Nat. Biotechnol. 2003, 21, 566-569.
14. Cooper, E. C., Jan, L. Y., Proc. Natl. Acad. Sci. USA 1999, 96, 4759-4766.
15. Bryant, N. J., Govers, R., James, D. E., Nat. Rev. Mol. Cell Biol. 2002, 3, 267-277.
16. Dreger, M., Eur. J. Biochem. 2003, 270, 569-569.
17. http://grimwade.biochem.unimelb.edu.au/bowtell/biochem/biochem.htm
18. Hartinger, J., Stenius, K., Hogemann, D., Jahn, R., Anal. Biochem. 1996, 240, 126-133.
19. Nothwang, H. G., Becker, M., Ociepka, K., Friauf, E., Brain Res. Mol. Brain Res. 2003, 116, 59-69.
20. http://www.ncbi.nim.nih.gov/BLAST/
21. http://www.geneontology.org/
22. http://us.expasy.org/sprot/
23. Croze, E. M., Morre, D. J., J. Cell. Physiol. 1984, 119, 46-57.
24. Fleischer, S., Kervina, M., Methods Enzymol. 1974, 31, 6-41.
25. Dreger, M., Bengtsson, L., Schoneberg, T., Otto, H. et al., Proc. Natl. Acad. Sci. USA 2001, 98, 11943-11948.
26. Walikonis, R. S., Jensen, O. N., Mann, M., Provance, D. W. et al., J. Neurosci. 2000, 20, 4069-4080.
27. Huh, W. K., Falvo, J. V., Gerke, L. C., Carroll, A. S. et al., Nature 2003, 425, 686-691.
28. Krapfenbauer, K., Fountoulakis, M., Lubec, G., Electrophoresis 2003, 24, 1847-1870.
29. Langen, H., Berndt, P., Roder, D., Cairns, N. et al., Electrophoresis 1999, 20, 907-916.
30. Laschet, J. J., Minier, F., Kurcewicz, I., Bureau, M. H. et al., J. Neurosci. 2004, 24, 7614-7622.
31. Diao, A., Rahman, D., Pappin, D. J. C., Lucocq, J. et al., J. Cell Biol. 2003, 160, 201-212.
32. Helenius, A., Aebi, M., Science 2001, 291, 2364-2369.
33. Shav-Tal, Y., Cohen, M., Lapter, S., Dye, B. et al., Mol. Biol. Cell 2001, 12, 2328-2340.
34. Bifulco, M., Laezza, C., Stingo, S., Wolff, J., Proc. Natl. Acad. Sci. USA 2002, 99, 1807-1812.