Quantification of Jκ signal end breaks in developing B cells by blunt-end linker ligation and qPCR

Journal of Immunological Methods

Volumn 296, Issue 1-2, 2005, Pages 19-30

  Curry, John D ^a   Li, Lydia ^a   Schlissel, Mark S ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Blunt ended ligation; dsDNA breaks; J kappa 1; Murine; Pre B cells; qPCR

Indexed keywords

DOUBLE STRANDED DNA; GENOMIC DNA; RECOMBINASE; DNA; DNA BINDING PROTEIN; IMMUNOGLOBULIN J RECOMBINATION SIGNAL SEQUENCE BINDING PROTEIN; IMMUNOGLOBULIN KAPPA CHAIN; NUCLEAR PROTEIN; RBPSUH PROTEIN, MOUSE;

ANIMAL CELL; ARTICLE; B LYMPHOCYTE; BONE MARROW CELL; CELL LINE; DNA STRAND BREAKAGE; GENE LOCUS; GENE REARRANGEMENT; HYBRIDIZATION; KINETICS; LIGATION; MOUSE; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY ANALYSIS; SIGNAL TRANSDUCTION; SOUTHERN BLOTTING; TEMPERATURE SENSITIVITY; ANIMAL; CHEMISTRY; DNA DAMAGE; GENETIC RECOMBINATION; GENETICS; IMMUNOGLOBULIN STRUCTURE; IMMUNOGLOBULIN VARIABLE REGION; IMMUNOLOGY; METHODOLOGY; PHYSIOLOGY;

MURINAE;

ANIMALS; B-LYMPHOCYTES; BONE MARROW CELLS; DNA; DNA DAMAGE; DNA-BINDING PROTEINS; GENE REARRANGEMENT, B-LYMPHOCYTE, LIGHT CHAIN; IMMUNOGLOBULIN J RECOMBINATION SIGNAL SEQUENCE-BINDING PROTEIN; IMMUNOGLOBULIN JOINING REGION; IMMUNOGLOBULIN KAPPA-CHAINS; IMMUNOGLOBULIN VARIABLE REGION; MICE; NUCLEAR PROTEINS; POLYMERASE CHAIN REACTION; RECOMBINATION, GENETIC; VDJ RECOMBINASES;

EID: 12944302100     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2004.10.003     Document Type: Article

References (15)

1. E.L. Beall, and D.C. Rio Drosophila P-element transposase is a novel site-specific endonuclease Genes Dev. 11 1997 2137
2. Y.Y. Chen, L.C. Wang, M.S. Huang, and N. Rosenberg An active v-abl protein tyrosine kinase blocks immunoglobulin light-chain gene rearrangement Genes Dev. 8 1994 688
3. A. Constantinescu, and M.S. Schlissel Changes in locus-specific V(D)J recombinase activity induced by immunoglobulin gene products during B cell development J. Exp. Med. 185 1997 609
4. M. Gellert V(D)J recombination: RAG proteins, repair factors, and regulation Annu. Rev. Biochem. 71 2002 101
5. L.Y. Hsu, J. Lauring, H.E. Liang, S. Greenbaum, D. Cado, Y. Zhuang, and M.S. Schlissel A conserved transcriptional enhancer regulates RAG gene expression in developing B cells Immunity 19 2003 105
6. D. Jung, and F.W. Alt Unraveling V(D)J recombination; insights into gene regulation Cell 116 2004 299
7. R. Kostriken, J.N. Strathern, A.J. Klar, J.B. Hicks, and F. Heffron A site-specific endonuclease essential for mating-type switching in Saccharomyces cerevisiae Cell 35 1983 167
8. H.E. Liang, L.Y. Hsu, D. Cado, L.G. Cowell, G. Kelsoe, and M.S. Schlissel The "dispensable" portion of RAG2 is necessary for efficient V-to-DJ rearrangement during B and T cell development Immunity 17 2002 639
9. P.R. Mueller, and B. Wold In vivo footprinting of a muscle specific enhancer by ligation mediated PCR Science 246 1989 780
10. P.D. Ponath, R.T. Boyd, D.M. Hillis, and P.D. Gottlieb Structural and evolutionary comparisons of four alleles of the mouse Igk-J locus which encodes immunoglobulin kappa light chain joining (J kappa) segments Immunogenetics 29 1989 389
11. D.A. Ramsden, and M. Gellert Formation and resolution of double-strand break intermediates in V(D)J rearrangement Genes Dev. 9 1995 2409
12. D.B. Roth, C. Zhu, and M. Gellert Characterization of broken DNA molecules associated with V(D)J recombination Proc. Natl. Acad. Sci. U. S. A. 90 1993 10788
13. M. Schlissel, A. Constantinescu, T. Morrow, M. Baxter, and A. Peng Double-strand signal sequence breaks in V(D)J recombination are blunt, 5′-phosphorylated. RAG-dependent, and cell cycle regulated Genes Dev. 7 1993 2520
14. M.S. Schlissel Structure of nonhairpin coding-end DNA breaks in cells undergoing V(D)J recombination Mol. Cell. Biol. 18 1998 2029
15. Y. Shinkai, G. Rathbun, K.P. Lam, E.M. Oltz, V. Stewart, M. Mendelsohn, J. Charron, M. Datta, F. Young, and A.M. Stall RAG-2-deficient mice lack mature lymphocytes owing to inability to initiate V(D)J rearrangement Cell 68 1992 855