Probing the mechanism of the bifunctional enzyme ketol-acid reductoisomerase by site-directed mutagenesis of the active site

FEBS Journal

Volumn 272, Issue 2, 2005, Pages 593-602

  Tyagi, Rajiv ^a   Lee, Yu Ting ^a   Guddat, Luke W ^a   Duggleby, Ronald G ^a  

^a UNIVERSITY OF QUEENSLAND   (Australia)

Author keywords

Alkyl migration reaction; Branched chain amino acids; Equilibrium constant; Ketoacid reductase; Transition state

Indexed keywords

ISOMERASE; OXIDOREDUCTASE; OXOACID; OXOACID REDUCTOISOMERASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; UNCLASSIFIED DRUG;

AMINO ACID ANALYSIS; ARTICLE; CATALYSIS; ENZYME ACTIVE SITE; ENZYME MECHANISM; ENZYME PURIFICATION; ENZYME SPECIFICITY; EQUILIBRIUM CONSTANT; ESCHERICHIA COLI; GENE MUTATION; ISOMERIZATION; NONHUMAN; PRIORITY JOURNAL; REACTION ANALYSIS; SITE DIRECTED MUTAGENESIS;

ALCOHOL OXIDOREDUCTASES; BINDING SITES; CATALYSIS; KETOL-ACID REDUCTOISOMERASE; KINETICS; MAGNESIUM; MUTAGENESIS, SITE-DIRECTED; NADP;

ESCHERICHIA COLI; NEGIBACTERIA;

EID: 12544251400     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/j.1742-4658.2004.04506.x     Document Type: Article

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