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note
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The apparatus for the passive avoidance test consists of a two-compartment acrylic box with a lighted compartment connected to a darkened one by a guillotine door. In the original method, mice received a punishing electrical shock as soon as they entered the dark compartment, while in our modified method, after entry into the dark compartment, mice receive a non-painful punishment consisting of a fall (from 40 cm) into a cold water bath (10°C). For this purpose, the dark chamber was constructed with a pitfall floor. The latency times for entering the dark compartment were measured in the training test (first day) and after 24 h in the retention test (second day). Mice who did not enter after 60 s latency were excluded from the experiment. For memory disruption, mice were ip injected with the amnesic drug scopolamine. All investigated drugs, dissolved in saline, were given ip 20 min before the training session, while amnesic drug was injected immediately after termination of the training session. The maximum entry latency allowed in the retention session was 120 s. The degree of received punishment memory (fall into cold water) was expressed as the increase in seconds between training and retention latencies.
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