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Volumn 13, Issue 18, 2003, Pages 3105-3110

Mapping the kinase domain of janus kinase 3

Author keywords

[No Author keywords available]

Indexed keywords

CYCLIC AMP DEPENDENT PROTEIN KINASE; INSULIN RECEPTOR KINASE; JANUS KINASE; OXINDOLE; PROTEIN SUBUNIT;

EID: 12444255147     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0960-894X(03)00657-7     Document Type: Article
Times cited : (44)

References (28)
  • 10
    • 85031073925 scopus 로고    scopus 로고
    • The protein was expressed with an N-terminal FLAG tag using the in-house expression vector pYOUNG and the yeast strain Kluveromycas lactis. The recombinant protein was expressed intracellularly and was purified using M2 affinity resin.
  • 11
    • 85031080896 scopus 로고    scopus 로고
    • max is determined using Packard discovery counter. The final DMSO concentration in the assay was 8%. The standard for the assay was oxindole 1.
  • 21
    • 85031077489 scopus 로고    scopus 로고
    • 85% by LC/MS, was set as the lower limit for acceptance of each library component.
  • 24
    • 85031078076 scopus 로고    scopus 로고
    • 3H]-thymidine incorporation into DNA reflects extent of proliferation.
  • 25
    • 85031075018 scopus 로고    scopus 로고
    • IL-2 induced JAK 3 and STAT5 tyrosine phosphorylation: Pretreat CTLL cells with JAK 3 inhibitors for 4 h. Stimulate cells with 10 U/mL IL-2 for 20 min, lyse cells and immunoprecipitate with anti-mouse JAK 3 and STAT5 antibodies. Subject immunoprecipitated samples to SDS-PAGE electrophoresis. Probe electrophoresized blots with anti-phosphotyrosine antibody.
  • 28
    • 85031080763 scopus 로고    scopus 로고
    • Protocol for oxazolone induced ear odema: On day 1, female BALB/c mice were sensitized by topical application of oxazolone (100 μL of 2% solution in acetone) to the shaved abdomen. On day 7, acetone (negative control group) or oxazolone (2% solution in acetone, remainder of animals) was applied topically to the right ear (10 μL on each side of the ear). Test compounds or appropriate vehicles (acetone) were administered topically to the right ear (10 μL on each side of the ear) 0.5 h after challenge. Mice were killed 24 h after challenge. A 6-mm disc of challenged (right) ear was removed and weighed.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.