Enhancing protein expression in single HEK 293 cells

Journal of Neuroscience Methods

Volumn 142, Issue 1, 2005, Pages 159-166

  Huang, Zhen ^a   Li, Gang ^a   Pei, Weimin ^a   Sosa, Leivi A ^a   Niu, Li ^a  

^a STATE UNIVERSITY OF NEW YORK   (United States)

Author keywords

Glutamate ion channels; Green fluorescent protein; HEK 293 cells; Protein expression; Simian virus 40 large T antigen; Transient transfection

Indexed keywords

GLUTAMATE RECEPTOR 2; GREEN FLUORESCENT PROTEIN; MEMBRANE PROTEIN; ONCOPROTEIN; VIRUS T ANTIGEN;

ARTICLE; CELL POPULATION; CELL STRAIN HEK293; CONTROLLED STUDY; EMBRYO; FLUORESCENCE; HUMAN; HUMAN CELL; PLASMID; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN FUNCTION; SIMIAN VIRUS 40;

ANTIGENS, VIRAL, TUMOR; CELL LINE; GENE EXPRESSION REGULATION; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HUMANS; KIDNEY; MEMBRANE POTENTIALS; MEMBRANE PROTEINS; MICROSCOPY, FLUORESCENCE; MOLECULAR BIOLOGY; NEUROCHEMISTRY; PATCH-CLAMP TECHNIQUES; PLASMIDS; RECEPTORS, AMPA; RECOMBINANT FUSION PROTEINS; UP-REGULATION;

EID: 12344307236     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2004.09.009     Document Type: Article

References (24)

1. S.H. Ali, and J.A. DeCaprio Cellular transformation by SV40 large T antigen: interaction with host proteins Semin Cancer Biol 11 1 2001 15 23
2. N. Armstrong, and E. Gouaux Mechanisms for activation and antagonism of an AMPA-sensitive glutamate receptor: crystal structures of the GluR2 ligand binding core Neuron 28 1 2000 165 181
3. D.T. Berg, D.B. McClure, and B.W. Grinnell High-level expression of secreted proteins from cells adapted to serum-free suspension culture Biotechniques 14 6 1993 972 978
4. J. Boulter, M. Hollmann, A. O'Shea-Greenfield, M. Hartley, E. Deneris, and C. Maron Molecular cloning and functional expression of glutamate receptor subunit genes Science 249 4972 1990 1033 1037
5. M. Chalfie, Y. Tu, G. Euskirchen, W.W. Ward, and D.C. Prasher Green fluorescent protein as a marker for gene expression Science 263 5148 1994 802 805
6. W. Chen, and W.C. Hahn SV40 early region oncoproteins and human cell transformation Histol Histopathol 18 2 2003 541 550
7. C. Chen, and H. Okayama High-efficiency transformation of mammalian cells by plasmid DNA Mol Cell Biol 7 8 1987 2745 2752
8. C.M. Corsaro, and M.L. Pearson Competence for DNA transfer of ouabain resistance and thymidine kinase: clonal variation in mouse L-cell recipients Somatic Cell Genet 7 5 1981 617 630
9. R. Dingledine, K. Borges, D. Bowie, and S.F. Traynelis The glutamate receptor ion channels Pharmacol Rev 51 1 1999 7 61
10. M.W. Fleck, E. Cornell, and S.J. Mah Amino-acid residues involved in glutamate receptor 6 kainate receptor gating and desensitization J Neurosci 23 4 2003 1219 1227
11. F.L. Graham, J. Smiley, W.C. Russell, and R. Nairn Characteristics of a human cell line transformed by DNA from human adenovirus type 5 J Gen Virol 36 1 1977 59 74
12. F.L. Graham, and A.J. van der Eb Transformation of rat cells by DNA of human adenovirus 5 Virology 54 2 1973 536 539
13. J. Grosskreutz, A. Zoerner, F. Schlesinger, K. Krampfl, R. Dengler, and J. Bufler Kinetic properties of human AMPA-type glutamate receptors expressed in HEK293 cells Eur J Neurosci 17 6 2003 1173 1178
14. O.P. Hamill, A. Marty, E. Neher, B. Sakmann, and F.J. Sigworth Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches Pflugers Arch 391 2 1981 85 100
15. P.R. Heath, and P.J. Shaw Update on the glutamatergic neurotransmitter system and the role of excitotoxicity in amyotrophic lateral sclerosis Muscle Nerve 26 4 2002 438 458
16. G. Li, R.E. Oswald, and L. Niu Channel-opening kinetics of GluR6 kainate receptor Biochemistry 42 42 2003 12367 12375
17. G. Li, W. Pei, and L. Niu Channel-opening kinetics of GluR2Q(flip) AMPA receptor: a laser-pulse photolysis study Biochemistry 42 42 2003 12358 12366
18. H. Luthman, and G. Magnusson High efficiency polyoma DNA transfection of chloroquine treated cells Nucl Acids Res 11 5 1983 1295 1308
19. M. Sena-Esteves, Y. Saeki, S.M. Camp, E.A. Chiocca, and X.O. Breakefield Single-step conversion of cells to retrovirus vector producers with herpes simplex virus-Epstein-Barr virus hybrid amplicons J Virol 73 12 1999 10426 10439
20. D.T. Simmons SV40 large T antigen functions in DNA replication and transformation Adv Virus Res 55 2000 75 134
21. B.W. Stillman, and Y. Gluzman Replication and supercoiling of simian virus 40 DNA in cell extracts from human cells Mol Cell Biol 5 8 1985 2051 2060
22. C.S. Sullivan, and J.M. Pipas T antigens of simian virus 40: molecular chaperones for viral replication and tumorigenesis Microbiol Mol Biol Rev 66 2 2002 179 202
23. J.B. Udgaonkar, and G.P. Hess Chemical kinetic measurements of a mammalian acetylcholine receptor by a fast-reaction technique Proc Natl Acad Sci USA 84 24 1987 8758 8762
24. P. Washbourne, and A.K. McAllister Techniques for gene transfer into neurons Curr Opin Neurobiol 12 5 2002 566 573