Clinical and environmental isolates of Legionella pneumophila serogroup 1 cannot be distinguished by sequence analysis of two surface protein genes and three housekeeping genes

Applied and Environmental Microbiology

Volumn 71, Issue 1, 2005, Pages 282-289

  Aurell, Helena ^a   Farge, Pierre ^a   Meugnier, Hélène ^a   Gouy, Manolo ^b   Forey, Françoise ^a   Lina, Gérard ^a   Vandenesch, François ^a   Etienne, Jerome ^a   Jarraud, Sophie ^a  

^a INSERM   (France)

^b UNIVERSITÉ LYON 1   (France)

Author keywords

[No Author keywords available]

Indexed keywords

GENE SEQUENCING; PATHOGENECITY; PHYLOGENETIC TREES; POLYMORPHISM;

ALDEHYDES; ELECTROPHORESIS; ENVIRONMENTAL IMPACT; ENZYMES; GENETIC ENGINEERING; MEDICAL IMAGING; RNA;

GENES;

ACONITATE HYDRATASE; ASPARTATE SEMIALDEHYDE DEHYDROGENASE; MACROPHAGE INFECTIVITY POTENTIATOR PROTEIN; MEMBRANE PROTEIN; RNA POLYMERASE BETA SUBUNIT; UNCLASSIFIED DRUG;

BACTERIUM;

ARTICLE; BACTERIUM ISOLATE; CONTROLLED STUDY; EPIDEMIOLOGICAL DATA; GENE AMPLIFICATION; GENE CLUSTER; GENETIC CODE; GENETIC POLYMORPHISM; HOUSEKEEPING GENE; LEGIONELLA PNEUMOPHILA; NONHUMAN; NUCLEOTIDE SEQUENCE; PATHOGENICITY; PHYLOGENETIC TREE; PULSED FIELD GEL ELECTROPHORESIS; SEQUENCE ANALYSIS; STRAIN DIFFERENCE;

BACTERIAL PROTEINS; BACTERIAL TYPING TECHNIQUES; BASE SEQUENCE; HOSPITALS; HUMANS; LEGIONELLA PNEUMOPHILA; LEGIONNAIRES' DISEASE; MOLECULAR SEQUENCE DATA; PHYLOGENY; SEQUENCE ANALYSIS, DNA; WATER MICROBIOLOGY; WATER SUPPLY;

BACTERIA (MICROORGANISMS); LEGIONELLA; LEGIONELLA PNEUMOPHILA; LEGIONELLA PNEUMOPHILA SEROGROUP 1; NEGIBACTERIA;

EID: 12244293378     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.71.1.282-289.2005     Document Type: Article

References (52)

1. Aurell, H., J. Etienne, F. Forey, M. Reyrolle, P. Girardo, P. Farge, B. Decludt, C. Campesese, F. Vandenesch, and S. Jarraud. 2003. Legionella pneumophila serogroup 1 strain Paris: distribution throughout France. J. Clin. Microbiol. 41:3320-3322.
2. Bumbaugh, A. C., E. A. McGraw, K. L. Page, R. K. Selander, and T. S. Whittam. 2002. Sequence polymorphism of dotA and mip alleles mediating invasion and intracellular replication of Legionella pneumophila. Curr. Microbiol. 44:314-322.
3. Cianciotto, N. P., J. M. Bangsborg, B. I. Eisenstein, and N. C. Engleberg. 1990. Identification of mip-like genes in the genus Legionella. Infect. Immun. 58:2912-2918.
4. Den Boer, J. W., E. P. Yzerman, J. Schellekens, K. D. Lettinga, H. C. Boshuizen, J. E. Van Steenbergen, A. Bosman, S. Van den Hof, H. A. Van Vliet, M. F. Peeters, R. J. Van Ketel, P. Speelman, J. L. Kool, and M. A. Conyn-Van Spaendonck. 2002. A large outbreak of Legionnaires' disease at a flower show, The Netherlands, 1999. Emerg. Infect. Dis. 8:37-43.
5. Doleans, A., H. Aurell, M. Reyrolle, G. Lina, J. Freney, F. Vandenesch, J. Etienne, and S. Jarraud. 2004. Clinical and environmental distributions of Legionella strains in France are different. J. Clin. Microbiol. 42:458-460.
6. Enright, M. C., and B. G. Spratt. 1999. Multilocus sequence typing. Trends Microbiol. 7:482-487.
7. Enright, M. C., and B. G. Spratt. 1998. A multilocus sequence typing scheme for Streptococcus pneumoniae: identification of clones associated with serious invasive disease. Microbiology 144:3049-3060.
8. Farris, J. S., M. Källersj, A. G. Kluge, and C. Bult. 1997. Testing significance of incongruence. Cladistics 10:315-319.
9. Fernandez, J. A., P. Lopez, D. Orozco, and J. Merino. 2002. Clinical study of an outbreak of Legionnaire's disease in Alcoy, southeastern Spain. Eur. J. Clin. Microbiol. Infect. Dis. 21:729-735.
10. Fry, N. K., S. Alexiou-Daniel, J. M. Bangsborg, S. Bernander, M. C. Pastoris, J. Etienne, B. Forsblom, V. Gaia, J. H. Helbig, D. Lindsay, P. C. Lück, C. Pelaz, S. A. Uldum, and T. G. Harrison. 1999. A multicenter evaluation of genotypic methods for the epidemiologic typing of Legionella pneumophila serogroup 1: results of a pan-European study. Clin. Microbiol. Infect. 5:462-477.
11. Gaia, V., N. K. Fry, T. G. Harrison, and R. Peduzzi. 2003. Sequence-based typing of Legionella pneumophila serogroup 1 offers the potential for true portability in legionellosis outbreak investigation. J. Clin. Microbiol. 41:2932-2939.
12. Grundmann, H., S. Hori, M. C. Enright, C. Webster, A. Tami, E. J. Feil, and T. Pitt. 2002. Determining the genetic structure of the natural population of Staphylococcus aureus: a comparison of multilocus sequence typing with pulsed-field gel electrophoresis, randomly amplified polymorphic DNA analysis, and phage typing. J. Clin. Microbiol. 40:4544-4546.
13. Grundmann, H., S. Hori, and G. Tanner. 2001. Determining confidence intervals when measuring genetic diversity and the discriminatory abilities of typing methods for microorganisms. J. Clin. Microbiol. 39:4190-4192.
14. Harb, O. S., L.-Y. Gao, and Y. A. Kwaik. 2000. From protozoa to mammalian cells: a new paradigm in the life cycle of intracellular bacterial pathogens. Environ. Microbiol. 2:251-265.
15. Heuner, K., M. Steinert, R. Marre, and J. Hacker. 2002. Genomic structure and evolution of Legionella species. Curr. Top. Microbiol. Immunol. 264:61-78.
16. Hunter, P. R., and M. A. Gaston. 1988. Numerical index of the discriminatory ability of typing systems: an application of Simpson's index of diversity. J. Clin. Microbiol. 26:2465-2466.
17. Jonas, D., H. G. Meyer, P. Matthes, D. Hartung, B. Jahn, F. D. Daschner, and B. Jansen. 2000. Comparative evaluation of three different genotyping methods for investigation of nosocomial outbreaks of Legionnaires' disease in hospitals. J. Clin. Microbiol. 38:2284-2291.
18. Ko, K. S., S. K. Hong, H. K. Lee, M. Y. Park, and Y. H. Kook. 2003. Mosaic structure of pathogenicity islands in Legionella pneumophila. J. Mol. Evol. 57:63-72.
19. Ko, K. S., S. K. Hong, H. K. Lee, M. Y. Park, and Y. H. Kook. 2003. Molecular evolution of the dotA gene in Legionella pneumophila. J. Bacteriol. 185:6269-6277.
20. Ko, K. S., H. K. Lee, M. Y. Park, M. S. Park, K. H. Lee, S. Y. Woo, Y. J. Yun, and Y. H. Kook. 2002. Population genetic structure of Legionella pneumophila inferred from RNA polymerase gene (rpoB) and DotA gene (dotA) sequences. J. Bacteriol. 184:2123-2130.
21. Kohler, R., J. Fanghanel, B. Konig, E. Luneberg, M. Frosch, J. U. Rahfeld, R. Hilgenfeld, G. Fischer, J. Hacker, and M. Steinert. 2003. Biochemical and functional analyses of the Mip protein: influence of the N-terminal half and of peptidylprolyl isomerase activity on the virulence of Legionella pneumophila. Infect. Immun. 71:4389-4397.
22. Korber, B. 2000. Signature and sequence variation analysis., p. 55-72. In A. G. Rodrigo and G. H. Learns (ed.), Computational analysis of HIV molecular sequences. Kluwer Academic Publishers, Dordrecht, The Netherlands.
23. Kotetishvili, M., O. C. Stine, A. Kreger, J. G. Morris, Jr., and A. Sulakvelidze. 2002. Multilocus sequence typing for characterization of clinical and environmental Salmonella strains. J. Clin. Microbiol. 40:1626-1635.
24. Krinos, C., A. S. High, and F. G. Rodgers. 1999. Role of the 25 kDa major outer membrane protein of Legionella pneumophila in attachment to U-937 cells and its potential as a virulence factor for chick embryos. J. Appl. Microbiol. 86:237-244.
25. Lawrence, C., M. Reyrolle, S. Dubrou, F. Forey, B. Decludt, C. Goulvestre, P. Matsiota-Bernard, J. Etienne, and C. Nauciel. 1999. Single clonal origin of a high proportion of Legionella pneumophila serogroup 1 isolates from patients and the environment in the area of Paris, France, over a 10-year period. J. Clin. Microbiol. 37:2652-2655.
26. Lück, P. C., J. H. Helbig, V. Günter, M. Assman, R. Blau, H. Koch, and M. Klepp. 1994. Epidemiologic investigation by macrorestriction analysis and by using monoclonal antibodies of nosocomial pneumonia caused by Legionella pneumophila serogroup 10. J. Clin. Microbiol. 32:2692-2697.
27. Luneberg, E., N. Zetzmann, D. Alber, Y. A. Knirel, O. Kooistra, U. Zahringer, and M. Frosch. 2000. Cloning and functional characterization of a 30 kb gene locus required for lipopolysaccharide biosynthesis in Legionella pneumophila. Int. J. Med. Microbiol. 290:37-49.
28. Maiden, M. C., J. A. Bygraves, E. Feil, G. Morelli, J. E. Russell, R. Urwin, Q. Zhang, J. Zhou, K. Zurth, D. A. Caugant, I. M. Feavers, M. Achtman, and B. G. Spratt. 1998. Multilocus sequence typing: a portable approach to the identification of clones within populations of pathogenic microorganisms. Proc. Natl. Acad. Sci. USA 95:3140-3145.
29. Montanaro-Punzengruber, J. C., L. Hicks, W. Meyer, and G. L. Gilbert. 1999. Australian isolates of Legionella longbeachae are not a clonal population. J. Clin. Microbiol. 37:3249-3254.
30. Nei, M., and T. Gojobori. 1986. Simple methods for estimating the numbers of synonymous and nonsynonymous nucleotide substitutions. Mol. Biol. Evol. 3:418-426.
31. Park, M. Y., K. S. Ko, H. K. Lee, M. S. Park, and Y. H. Kook. 2003. Legionella busanensis sp. nov., isolated from cooling tower water in Korea. Int. J. Syst. Evol. Microbiol. 53:77-80.
32. Perriere, G., and M. Gouy. 1996. WWW-query: an on-line retrieval system for biological sequence banks. Biochimie 78:364-369.
33. Pourcel, C., Y. Vidgop, F. Ramisse, G. Vergnaud, and C. Tram. 2003. Characterization of a tandem repeat polymorphism in Legionella pneumophila and its use for genotyping. J. Clin. Microbiol. 41:1819-1826.
34. Pruckler, J. M., L. A. Mermel, R. F. Benson, C. Giorgio, P. K. Cassiday, R. F. Breiman, C. G. Whitney, and B. S. Fields. 1995. Comparison of Legionella pneumophila isolates by arbitrarily primed PCR and pulsed-field gel electrophoresis: analysis from seven epidemic investigations. J. Clin. Microbiol. 33:2872-2875.
35. Rangel-Frausto, M. S., P. Rhomberg, R. J. Hollis, M. A. Pfaller, R. P. Wenzel, C. M. Helms, and L. A. Herwaldt. 1999. Persistence of Legionella pneumophila in a hospital's water system: a 13-year survey. Infect. Control Hosp. Epidemiol. 20:793-797.
36. Ratcliff, R. M., J. A. Lanser, P. A. Manning, and M. W. Heuzenroeder. 1998. Sequence-based classification scheme for the genus Legionella targeting the mip gene. J. Clin. Microbiol. 36:1560-1567.
37. Riboldi-Tunnicliffe, A., B. Konig, S. Jessen, M. S. Weiss, J. Rahfeld, J. Hacker, G. Fischer, and R. Hilgenfeld. 2001. Crystal structure of Mip, a prolylisomerase from Legionella pneumophila. Nat. Struct. Biol. 8:779-783.
38. Riffard, S., F. Lo Presti, F. Vandenesch, F. Forey, M. Reyrolle, and J. Etienne. 1998. Comparative analysis of infrequent-restriction-site PCR and pulsed-field gel electrophoresis for epidemiological typing of Legionella pneumophila serogroup 1 strains. J. Clin. Microbiol. 36:161-167.
39. Robinson, D. A., D. E. Briles, M. J. Crain, and S. K. Hollingshead. 2002. Evolution and virulence of serogroup 6 pneumococci on a global scale. J. Bacteriol. 184:6367-6375.
40. Salcedo, C., L. Arreaza, B. Alcala, L. De La Fuente, and J. A. Vazquez. 2003. Development of a multilocus sequence typing method for analysis of Listeria monocytogenes clones. J. Clin. Microbiol. 41:757-762.
41. Schoonmaker, D., T. Heimberger, and G. Birkhead. 1992. Comparison of ribotyping and restriction enzyme analysis using pulsed-field gel electrophoresis for distinguishing Legionella pneumophila isolates obtained during a nosocomial outbreak. J. Clin. Microbiol. 30:1491-1498.
42. Segal, G., J. J. Russo, and H. A. Shuman. 1999. Relationships between a new type IV secretion system and the icm/dot virulence system of Legionella pneumophila. Mol. Microbiol. 34:799-809.
43. Selander, R. K., R. M. McKinney, T. S. Whittam, W. F. Bibb, D. J. Brenner, F. S. Nolte, and P. E. Pattison. 1985. Genetic structure of populations of Legionella pneumophila. J. Bacteriol. 163:1021-1037.
44. Simpson, E. H. 1949. Measurement of diversity. Nature 163:688.
45. Smith, J. M., N. H. Smith, M. O'Rourke, and B. G. Spratt. 1993. How clonal are bacteria? Proc. Natl. Acad. Sci. USA 90:4384-4388.
46. Spratt, B. G. 1999. Multilocus sequence typing: molecular typing of bacterial pathogens in an era of rapid DNA sequencing and the internet. Curr. Opin. Microbiol. 2:312-316.
47. Struelens, M. J. 1996. Consensus guidelines for appropriate use and evaluation of microbial epidemiologic typing systems. Clin. Microbiol. Infect. 2:2-11.
48. Swofford, D. L. 1999. PAUP*: phylogenetic analysis using parsimony (*and other methods), version 4. Sinauer Associates, Sunderland, Mass.
49. Thompson, J. D., T. J. Gibson, F. Plewniak, F. Jeanmougin, and D. G. Higgins. 1997. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 25:4876-4882.
50. Visca, P., P. Goldoni, P. C. Lück, J. H. Helbig, L. Cattani, G. Giltri, S. Bramati, and M. C. Pastoris. 1999. Multiple types of Legionella pneumophila serogroup 6 in a hospital heated-water system associated with sporadic infections. J. Clin. Microbiol. 37:2189-2196.
51. Wintermeyer, E., B. Ludwig, M. Steinert, B. Schmidt, G. Fischer, and J. Hacker. 1995. Influence of site specifically altered Mip proteins on intracellular survival of Legionella pneumophila in eukaryotic cells. Infect. Immun. 63:4576-4583.
52. Yu, V. L., J. F. Plouffe, M. C. Pastoris, J. E. Stout, M. Schousboe, A. Widmer, J. Summersgill, T. File, C. M. Heath, D. L. Paterson, and A. Chereshsky. 2002. Distribution of Legionella species and serogroups isolated by culture in patients with sporadic community-acquired legionellosis: an international collaborative survey. J. Infect. Dis. 186:127-128.