Futile cycling of lactate through the plasma membrane of C6 glioma cells as detected by (13C, 2H) NMR

Journal of Neuroscience Research

Volumn 79, Issue 1-2, 2005, Pages 119-127

  Rodrigues, Tiago B ^a,b   Gray, Heather L ^a   Benito, Marina ^a   Garrido, Susana ^a   Sierra, Alejandra ^a   Geraldes, Carlos F ^b   Ballesteros, Paloma ^c   Cerdán, Sebastián ^a  

^a INSTITUTO DE INVESTIGACIONES BIOMÉDICAS ALBERTO SOLS   (Spain)

^b UNIVERSITY OF COIMBRA   (Portugal)

^c UNIVERSIDAD NACIONAL DE EDUCACIÓN A DISTANCIA   (Spain)

Author keywords

13C NMR; C6 cells; Lactate recycling; MCT1; Monocarboxylate transporter

Indexed keywords

GLUCOSE; LACTATE DEHYDROGENASE; LACTIC ACID; MONOCARBOXYLATE TRANSPORTER; PYRUVIC ACID;

ANIMAL CELL; CARBON NUCLEAR MAGNETIC RESONANCE; CELL CULTURE; CELL MEMBRANE; CONFERENCE PAPER; CONTROLLED STUDY; DEUTERON NUCLEAR MAGNETIC RESONANCE; GLIOMA CELL; GLYCOLYSIS; NONHUMAN; OXIDATION REDUCTION REACTION; PRIORITY JOURNAL; RAT;

ANIMALS; CARBON ISOTOPES; CELL LINE; CELL MEMBRANE; CITRIC ACID CYCLE; GLIOMA; GLUCOSE; ISOTOPES; L-LACTATE DEHYDROGENASE; LACTIC ACID; MAGNETIC RESONANCE SPECTROSCOPY; MICE; PENTOSE PHOSPHATE PATHWAY; PYRUVIC ACID; SUBSTRATE CYCLING; TIME FACTORS; TROMETHAMINE;

EID: 12244291011     PISSN: 03604012     EISSN: None     Source Type: Journal    
DOI: 10.1002/jnr.20308     Document Type: Conference Paper

References (37)

1. Bergmeyer HU. 1983. Methods of enzymatic analysis: Weinheim: Verlag Chemie.
2. 1H NMR study. J Biol Chem 273:27162-27169.
3. Bouzier AK, Voisin P, Goodwin R, Canioni P, Merle M. 1998b. Glucose and lactate metabolism in C6 glioma cells: evidence for the preferential utilization of lactate for cell oxidative metabolism. Dev Neurosci 20:331-338.
4. Bouzier-Sore AK, Canioni P, Merle M. 2001. Effect of exogenous lactate on rat glioma metabolism. J Neurosci Res 65:543-548.
5. Bouzier-Sore AK, Voisin P, Canioni P, Magistretti TJ, Pellerin T. 2003. Lactate is a preferential oxidative energy substrate over glucose for neurons in culture. J Cereb Blood Flow Metab 23:1298-1306.
6. Cater HL, Chandratheva A, Benham CD, Morrison B, Sundstrom LE. 2003. Lactate and glucose as energy substrates during, and after, oxygen deprivation in rat hippocampal acute and cultured slices. J Neurochem 87:1381-1390.
7. 13C NMR. J Biol Chem 265:12916-12926.
8. 2H] NMR spectroscopy. Neurochem Int 37:217-228.
9. 13C labels. J Neurosci Res 66:771-781.
10. Dienel GA, Cruz NF. 2003. Neighborly interactions of metabolically- activated astrocytes in vivo. Neurochem Int 43:339-354.
11. Dienel GA, Hertz L. 2001. Glucose and lactate metabolism during brain activation. J Neurosci Res 66:824-838.
12. Fan TWM. 1996. Metabolite profiling by one- and two-dimensional NMR analysis of complex mixtures. Prog NMR Spectrosc 28:161-219.
13. 13C-NMR spectroscopy. Sixth International Meeting for Brain Energy Metabolism. Heraklion, Crete, Greece. p 48.
14. 13C NMR. NMR Biomed 16:440-449.
15. 13C NMR spectroscopy. Neurochem Int 45:297-303.
16. García-Martín ML, Ballesteros P, Cerdán S. 2001a. The metabolism of water in cells and tissues as detected by NMR methods. Prog NMR Spectrosc 39:41-77.
17. 13C NMR. J Biol Chem 277:7799-7807.
18. 1H magnetic resonance spectroscopic imaging: comparison with maps of metabolites. Cancer Res 61:6524-6531.
19. Halestrap AP, Meredith D. 2004. The SLC16 gene family-from monocarboxylate transporters (MCTs) to aromatic amino acid transporters and beyond. Pflugers Arch 447:619-628.
20. Halestrap AP, Price NT. 1999. The proton-linked monocarboxylate transporter (MCT) family: structure, function and regulation. Biochem J 343:281-299.
21. Hansen PE. 1983. Isotope effects on nuclear shielding. Ann Rep NMR Spectrosc 15:105-234.
22. Hansen PE. 1988. Deuterium isotope effects on nuclear shielding. Progr NMR Spectrosc 20:207-257.
23. Jackson VN, Halestrap AP. 1996. The kinetics, substrate, and inhibitor specificity of the monocarboxylate (lactate) transporter of rat liver cells determined using the fluorescent intracellular pH indicator, 2′,7′-bis(carboxyethyl)-5(6)-carboxyfluorescein. J Biol Chem 271:861-868.
24. Magistretti PJ, Pellerin L. 1997. The cellular bases of functional brain imaging: evidence for astrocyte-neuron metabolic coupling. Neuroscientist 3:361-365.
25. Magistretti PJ, Pellerin L. 1999. Astrocytes couple synaptic activity to glucose utilization in the brain. News Physiol Sci 14:177-182.
26. 2O as detected by multinuclear NMR spectroscopy. NMR Biomed 7:249-262.
27. 13C NMR spectroscopy study of cortical nerve cell cultures exposed to hypoxia. J Neurosci Res 38:319-326.
28. Pellerin L. 2003. Lactate as a pivotal element in neuron-glia metabolic cooperation. Neurochem Int 43:331-338.
29. Pellerin L, Bonvento G, Chatton JY, Pierre K, Magistretti PJ. 2002. Role of neuron-glia interaction in the regulation of brain glucose utilization. Diabetes Nutr Metab 15:268-273.
30. Pellerin L, Magistretti PJ. 2004. Neuroenergetics: calling upon astrocytes to satisfy hungry neurons. Neuroscientist 10:53-62.
31. Perrin A, Roudier E, Duborjal H, Bachelet C, Riva-Lavieille C, Leverve X, Massarelli R. 2002. Pyruvate reverses metabolic effects produced by hypoxia in glioma and hepatoma cell cultures. Biochimie 84:1003-1011.
32. Poole RC, Halestrap AP. 1993. Transport of lactate and other monocarboxylates across mammalian plasma membranes. Am J Physiol 264:761-782.
33. 13)C MR spectroscopy study of lactate as substrate for rat brain. Dev Neurosci 22:429-436.
34. Schurr A. 2002. Energy metabolism, stress hormones and neural recovery from cerebral ischemia/hypoxia. Neurochem Int 41:1-8.
35. Schurr A, Payne RS, Miller JJ, Tseng MT. 2001. Preischemic hyperglycemia-aggravated damage: evidence that lactate utilization is beneficial and glucose-induced corticosterone release is detrimental. J Neurosci Res 66:782-789.
36. Smith D, Pernet A, Hallett WA, Bingham E, Marsden PK, Amiel SA. 2003. Lactate: a preferred fuel for human brain metabolism in vivo. J Cereb Blood Flow Metab 23:658-664.
37. 13C isotopomer analysis of glucose and alanine metabolism reveals cytosolic pyruvate compartmentation as part of energy metabolism in astrocytes. Glia 34:200-212.