Development of Taqman RT-nested PCR system for clinical SARS-CoV detection

Journal of Virological Methods

Volumn 119, Issue 1, 2004, Pages 17-23

  Wu, Qingfa ^a   Xu, Zuyuan ^a   Wei, Tian ^b,c   Zeng, Haipang ^a   Li, Jingxiang ^a   Gang, Haixue ^a   Sun, Min ^a   Jiang, Fangbo ^a   Wang, Xiang ^c   Dong, Wei ^a   Yang, Ling ^a   Wang, Jian ^a  

^a INSTITUTE OF GEOLOGY AND GEOPHYSICS   (China)

^b XI'AN JIAOTONG UNIVERSITY   (China)

^c Hangzhou Genomics Institute   (China)

Author keywords

Nested PCR; SARS; Sensitivity; Specificity; Taqman probe

Indexed keywords

PRIMER RNA;

ADULT; AMPLICON; ARTICLE; BLOOD ANALYSIS; BLOOD SAMPLING; CLINICAL ARTICLE; COMPARATIVE STUDY; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DIAGNOSTIC TEST; EARLY DIAGNOSIS; FEMALE; HUMAN; MALE; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ISOLATION; SARS CORONAVIRUS; SEVERE ACUTE RESPIRATORY SYNDROME; VERO CELL; VIRUS CULTURE; VIRUS DETECTION; VIRUS DIAGNOSIS; VIRUS GENOME; VIRUS IDENTIFICATION; VIRUS ISOLATION;

ADOLESCENT; ADULT; AGED; BASE SEQUENCE; CHILD; CHINA; DNA PRIMERS; DNA, VIRAL; FEMALE; GENOME, VIRAL; HUMANS; MALE; MIDDLE AGED; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SARS VIRUS; SENSITIVITY AND SPECIFICITY; SEVERE ACUTE RESPIRATORY SYNDROME; VIROLOGY;

CORONAVIRUS;

EID: 11144354043     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2004.02.011     Document Type: Article

References (11)

1. Drosten C., Gunther S., Preiser W., van der Werf S., Brodt H.R., Becker S., Rabenau H., Panning M., Kolesnikova L., Fouchier R.A., Berger A., Burguiere A.M., Cinatl J., Eickmann M., Escriou N., Grywna K., Kramme S., Manuguerra J.C., Muller S., Rickerts V., Sturmer M., Vieth S., Klenk H.D., Osterhaus A.D., Schmitz H., Doerr H.W. Identification of a novel coronavirus in patients with severe acute respiratory syndrome. N. Engl. J. Med. 348(20):2003;1967-1976.
2. Fortin N.Y., Mulchandani A., Chen W. Use of real-time polymerase chain reaction and molecular beacons for the detection of Escherichia coli O157:H7. Anal. Biochem. 289:2001;281-288.
3. Heid C.A., Stevens J., Livak K.J., Williams P.M. Real time quantitative PCR. Genome Res. 6(10):1996;986-994.
4. Ksiazek T.G., Erdman D., Goldsmith C.S., Zaki S.R., Peret T., Emery S., Tong S., Urbani C., Comer J.A., Lim W., Rollin P.E., Dowell S.F., Ling A.E., Humphrey C.D., Shieh W.J., Guarner J., Paddock C.D., Rota P., Fields B., DeRisi J., Yang J.Y., Cox N., Hughes J.M., LeDuc J.W., Bellini W.J., Anderson L.J. SARS Working Group A novel coronavirus associated with severe acute respiratory syndrome. N. Engl. J. Med. 348(20):2003;1953-1966.
5. Marra M.A., Jones S.J., Astell C.R., Holt R.A., Brooks-Wilson A., Butterfield Y.S., Khattra J., Asano J.K., Barber S.A., Chan S.Y., Cloutier A., Coughlin S.M., Freeman D., Girn N., Griffith O.L., Leach S.R., Mayo M., McDonald H., Montgomery S.B., Pandoh P.K., Petrescu A.S., Robertson A.G., Schein J.E., Siddiqui A., Smailus D.E., Stott J.M., Yang G.S., Plummer F., Andonov A., Artsob H., Bastien N., Bernard K., Booth T.F., Bowness D., Czub M., Drebot M., Fernando L., Flick R., Garbutt M., Gray M., Grolla A., Jones S., Feldmann H., Meyers A., Kabani A., Li Y., Normand S., Stroher U., Tipples G.A., Tyler S., Vogrig R., Ward D., Watson B., Brunham R.C., Krajden M., Petric M., Skowronski D.M., Upton C., Roper R.L. The genome sequence of the SARS-associated coronavirus. Science. 300(5624):2003;1399-1404.
6. Poon L.L., Wong O.K., Chan K.H., Luk W., Yuen K.Y., Peiris J.S., Guan Y. Rapid diagnosis of a coronavirus associated with severe acute respiratory syndrome (SARS). Clin. Chem. 49(6):2003;953-955.
7. Peiris J.S., Lai S.T., Poon L.L., Guan Y., Yam L.Y., Lim W., Nicholls J., Yee W.K., Yan W.W., Cheung M.T., Cheng V.C., Chan K.H., Tsang D.N., Yung R.W., Ng T.K., Yuen K.Y. SARS Study Group Coronavirus as a possible cause of severe acute respiratory syndrome. Lancet. 361:2003;1319-1325.
8. Qin E., Zhu Q., Yu M., Fan B., Chang G., Si B., Yang B., Peng W., Jiang T., Liu B., Deng Y., Liu H., Zhang Y., Wang C., Li Y., Gan Y., Li X., Lv F., Tan G., Cao W., Yang R., Wang J., Li W., Xu Z., Li Y., Wu Q., Lin W., Cheng W., Tang L., Deng Y., Han Y., Li C., Lei M., Li G., Li W., Lv H., Shi J., Tong Z., Zhang F., Li S., Liu B., Liu S., Dong W., Wang J., Gane K.S.W., Yu J., Yang H. A complete sequence and comparative analysis of a SARS-associated virus (isolate BJ01). Chin. Sci. Bull. 48:2003;941-948.
9. Rosling L., Rosling M. Pneumonia causes panic in Guangdong Province. BMJ. 326(7386):2003;416.
10. Rota P.A., Oberste M.S., Monroe S.S., Nix W.A., Campagnoli R., Icenogle J.R., Penaranda S., Bankamp B., Maher K., Chen M.H., Tong S., Tamin A., Lowe L., Frace M., DeRisi J.L., Chen Q., Wang D., Erdman D.D., Peret T.C., Burns C., Ksiazek T.G., Rollin P.E., Sanchez A., Liffick S., Holloway B., Limor J., McCaustland K., Olsen-Rasmussen M., Fouchier R., Gunther S., Osterhaus A.D., Drosten C., Pallansch M.A., Anderson L.J., Bellini W.J. Characterization of a novel coronavirus associated with severe acute respiratory syndrome. Science. 300(5624):2003;1394-1399.
11. Ruan Y.J., Wei C.L., Ee A.L., Vega V.B., Thoreau H., Su S.T., Chia J.M., Ng P., Chiu K.P., Lim L., Zhang T., Peng C.K., Lin E.O., Lee N.M., Yee S.L., Ng L.F., Chee R.E., Stanton L.W., Long P.M., Liu E.T. Comparative full-length genome sequence analysis of 14 SARS coronavirus isolates and common mutations associated with putative origins of infection. Lancet. 361(9371):2003;1779-1785.