Chemistry in living cells: Detection of active proteasomes by a two-step labeling strategy

Angewandte Chemie - International Edition

Volumn 42, Issue 31, 2003, Pages 3626-3629

  Ovaa, Huib ^b   Van Swieten, Paul F ^a   Kessler, Benedikt M ^b   Leeuwenburgh, Michiel A ^a   Fiebiger, Edda ^b   Van den Nieuwendijk, Adrianus M C H ^a   Galardy, Paul J ^b   Van der Marel, Gijsbert A ^a   Ploegh, Hidde L ^b   Overkleeft, Herman S ^a  

^a LEIDEN UNIVERSITY   (Netherlands)

^b HARVARD MEDICAL SCHOOL   (United States)

Author keywords

Bioorganic chemistry; Inhibitors; Peptides; Proteomics; Solid phase synthesis

Indexed keywords

CATALYSIS; PROTEINS;

PROTEASOMES;

CELLS;

BIOTIN; PROTEASOME;

ARTICLE; CATALYSIS; CELL LABELING; CELL MEMBRANE PERMEABILITY; CYTOLYSIS; ENZYME ACTIVE SITE; ENZYME ACTIVITY; HUMAN; IN VIVO STUDY; MOLECULAR PROBE; POLYACRYLAMIDE GEL ELECTROPHORESIS;

AFFINITY LABELS; ANIMALS; BLOTTING, WESTERN; CHEMILUMINESCENT MEASUREMENTS; CYSTEINE ENDOPEPTIDASES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME INHIBITORS; HUMANS; MOLECULAR STRUCTURE; MULTIENZYME COMPLEXES; PROTEASOME ENDOPEPTIDASE COMPLEX; TUMOR CELLS, CULTURED;

EID: 10744221240     PISSN: 14337851     EISSN: None     Source Type: Journal    
DOI: 10.1002/anie.200351314     Document Type: Article

References (19)

1. A. Borodovsky, H. Ovaa, N. Kolli, T. Gan-Erdene, K. D. Wilkinson, H. L. Ploegh, B. M. Kessler, Chem. Biol. 2002, 9, 1149-1159.
2. D. C. Greenbaum, W. D. Arnold, F. Lu, L. Hayrapetian, A. Baruch, J. Krumrine, S. Toba, K. Chehade, D. Brömme, I. D. Kuntz, M. Bogyo, Chem. Biol. 2002, 9, 1085-1094.
3. G. C. Adam, E. J. Sorensen, B. F. Cravatt, Nat. Biotechnol. 2002, 20, 805-809.
4. G. C. Adam, B. F. Cravatt, E. J. Sorensen, Chem. Biol. 2001, 8, 81-95.
5. A. M. Lennon-Duménil, A. H. Bakker, R. Maehr, E. Fiebiger, H. S. Overkleeft, M. Rosemblatt, H. L. Ploegh, C. Lagaudrière-Gesbert, J. Exp. Med. 2002, 196, 529-540.
6. B. M. Kessler, D. Tortorella, M. Altun, A. F. Kisselev, E. Fiebiger, B. G. Hekking, H. L. Ploegh, H. S. Overkleeft, Chem. Biol. 2001, 8, 913-929.
7. T. Nazif, M. Bogyo, Proc. Natl. Acad. Sci. USA 2001, 98, 2967-2972.
8. K. L. Rock, A. L. Goldberg, Annu. Rev. Immunol. 1999, 17, 739-797.
9. 125I-Tyr or biocytin abolishes cell permeability and, in case of biocytin, also partially impairs its inhibition profile.
10. E. Saxon, C. R. Bertozzi, Science 2000, 287, 2007-2010.
11. K. L. Kiick, E. Saxon, D. A. Tirrell, C. R. Bertozzi, Proc. Natl. Acad. Sci. USA 2002, 99, 19-24.
12. J. T Lundquist, J. C. Pelletier, Org. Lett. 2001, 3, 781-783.
13. M. Bogyo, J. S. McMaster, M. Gaczynska, D. Tortorella, A. L. Goldberg, H. L. Ploegh, Proc. Natl. Acad. Sci. USA 1997, 94, 6629-6634.
14. L. Bourel, O. Carion, H. Gras-Masse, O. Melnyk, J. Pept. Sci. 2000, 6, 264-270.
15. S. W. Yeong, D. F. O'Brien, J. Org. Chem. 2001, 66, 4799-4802.
16. N. P. Dantuma, K. Lindsten, R. Glas, M. Jellne, M. G. Masucci, Nat. Biotechnol. 2000, 18, 538-543.
17. T. Hideshima, K. C. Anderson, Nat. Rev. Cancer 2002, 2, 927-937.
18. C. Soto, Nat. Rev. Neurosci. 2003, 4, 49-60.
19. After this manuscript had been submitted, the latent in vivo labeling of serine proteases was reported: A. E. Speers, G. C. Adam, B. F. Cravatt, J. Am. Chem. Soc. 2003, 125, 4686-4687.