Denaturing gradient gel electrophoresis polymorphism for rapid 16S rDNA clone screening and microbial diversity study

Journal of Bioscience and Bioengineering

Volumn 93, Issue 1, 2002, Pages 101-103

  Liu, Wen Tso ^a   Huang, Chun Lin ^a   Song, Liangfa ^b  

^a NATIONAL CENTRAL UNIVERSITY   (Taiwan)

^b NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

16S rRNA; Clone library; Community structure; DGGE; RFLP

Indexed keywords

CLONING; ELECTROPHORESIS; GELS; SCREENING;

GEL ELECTROPHORESIS;

DNA;

DNA 16S; DNA FRAGMENT; PRIMER DNA; RIBOSOME DNA;

ARTICLE; DENATURING GRADIENT GEL ELECTROPHORESIS; DNA SCREENING; GENE AMPLIFICATION; GENE INSERTION; MICROBIAL DIVERSITY; MOLECULAR CLONING; NONHUMAN; PHYLOGENY; POLYMERASE CHAIN REACTION; REPRODUCIBILITY; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; SLUDGE;

INSERTION SEQUENCES;

EID: 0036189216     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1389-1723(02)80063-9     Document Type: Article

References (12)

1. Phylo-genetic identification and in situ detection of individual microbial cells without cultivation
2. Novel division level bacterial diversity in a yellow-stone hot spring
3. A computer-simulated restriction fragment length polymorphism analysis of bacterial small-subunit rRNA genes: Efficacy of selected tetrameric restriction enzymes for studies of microbial diversity in nature
4. Phylogenetic analysis and in situ identification of bacteria in activated sludge
5. Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology
6. Characterization of microbial diversity by determining terminal restriction fragment length polymorphisms of genes encoding 16S rRNA
7. In situ identification of polyphosphate- and polyhydroxyalkanoate-accumulating traits for microbial populations in a biological phosphorous removal process
8. Bacterial community structures of phosphate-removing and non-phosphate-removing and non-phosphate-removing activated sludges from sequencing batch reactors
9. Analysis of actinomycete communities by specific amplification of genes encoding 16S rRNA and gel-electrophoretic separation in denaturing gradients
10. Denaturing gradient gel electrophoresis profiles of 16S rRNA-defined populations inhabiting a hot spring microbial mat community
11. Microbial community changes in biological phosphate-removal systems on altering sludge phosphorus content
12. Estimation of diversity and community structure through restriction fragment length polymorphism distribution analysis of bacterial 16S rRNA genes from a microbial mat at an active, hydrothermal vent system, Loihi Seamount, Hawaii