Construction and characterization of transposon TnphoZ for the identification of genes encoding exported proteins in Streptococcus agalactiae

FEMS Microbiology Letters

Volumn 241, Issue 2, 2004, Pages 257-264

  Clancy, Anne ^a   Lee, Martin H ^a   Jones, Amanda L ^a   Rubens, Craig E ^a  

^a Landstuhl Regional Medical Center   (United States)

Author keywords

Alkaline phosphatase; Exported proteins; Group B streptococcus; Tn917; TnphoZ

Indexed keywords

ALKALINE PHOSPHATASE; BACTERIAL PROTEIN;

ARTICLE; BACTERIUM MUTANT; CONTROLLED STUDY; CORRELATION ANALYSIS; DNA FLANKING REGION; ENTEROCOCCUS FAECALIS; ENZYME ACTIVITY; ENZYME RELEASE; GENE CONSTRUCT; GENE EXPRESSION; GENE FUSION; GENE IDENTIFICATION; GENE INSERTION; GENE ISOLATION; GENE SEQUENCE; GENETIC CODE; GENETIC TRAIT; HOST; LONG TERMINAL REPEAT; NONHUMAN; NUCLEOTIDE SEQUENCE; PREDICTION; PRIORITY JOURNAL; STREPTOCOCCUS AGALACTIAE; TRANSPOSON;

ALKALINE PHOSPHATASE; AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BASE SEQUENCE; DNA TRANSPOSABLE ELEMENTS; ENTEROCOCCUS FAECALIS; GENETIC TECHNIQUES; HUMANS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, INSERTIONAL; PLASMIDS; RECOMBINANT FUSION PROTEINS; SEQUENCE ANALYSIS, DNA; STREPTOCOCCUS AGALACTIAE;

BACTERIA (MICROORGANISMS); ENTEROCOCCUS; ENTEROCOCCUS FAECALIS; INSERTION SEQUENCES; POSIBACTERIA; STREPTOCOCCUS; STREPTOCOCCUS AGALACTIAE; STREPTOCOCCUS SP. 'GROUP B'; TRANSPOSONS;

EID: 10444251038     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.femsle.2004.10.029     Document Type: Article

References (38)

1. A. Schuchat Epidemiology of group B streptococcal disease in the United States: shifting paradigms Clin. Microbiol. Rev. 11 1998 497 513
2. M. Moore, S. Schrag, and A. Schuchat Effects of intrapartum antimicrobial prophylaxis for prevention of group-B-streptococcal disease on the incidence and ecology of early-onset neonatal sepsis Lancet Infect. Dis. 3 2003 201 213
3. M.M. Farley Group B streptococcal disease in nonpregnant adults Clin. Infect. Dis. 33 2001 556 561
4. B. Spellerberg, E. Rozdzinski, S. Martin, J. Weber-Heynemann, N. Schnitzler, R. Lutticken, and A. Podbielski Lmb, a protein with similarities to the LraI adhesin family, mediates attachment of Streptococcus agalactiae to human laminin Infect. Immun. 67 1999 871 878
5. K.S. Doran, J.C. Chang, V.M. Benoit, L. Eckmann, and V. Nizet Group B streptococcal beta-hemolysin/cytolysin promotes invasion of human lung epithelial cells and the release of interleukin-8 J. Infect. Dis. 185 2002 196 203
6. M. Marques, D. Kasper, M. Pangburn, and M. Wessels Prevention of C3 deposition by capsular polysaccharide is a virulence mechanism of type III group B streptococci Infect. Immun. 60 1992 3986 3993
7. A. Jones, R.H. Needham, A. Clancy, K.M. Knoll, and C.E. Rubens Penicillin-binding proteins in Streptococcus agalactiae: a novel mechanism for evasion of immune clearance Mol. Microbiol. 47 2003 247 256
8. G.Y. Liu, and V. Nizet Extracellular virulence factors of group B Streptococci Front. Biosci. 1 2004 1794 1802
9. M.A. Herbert, C.J. Beveridge, and N.J. Saunders Bacterial virulence factors in neonatal sepsis: group B streptococcus Curr. Opin. Infect. Dis. 17 2004 225 229
10. E.E. Adderson, S. Takahashi, Y. Wang, J. Armstrong, D.V. Miller, and J.F. Bohnsack Subtractive hybridization identifies a novel predicted protein mediating epithelial cell invasion by virulent serotype III group B Streptococcus agalactiae Infect. Immun. 71 2003 6857 6863
11. H. Tettelin, V. Masignani, M.J. Cieslewicz, J.A. Eisen, S. Peterson, M.R. Wessels, I.T. Paulsen, K.E. Nelson, I. Margarit, T.D. Read, L.C. Madoff, A.M. Wolf, M.J. Beanan, L.M. Brinkac, S.C. Daugherty, R.T. DeBoy, A.S. Durkin, J.F. Kolonay, R. Madupu, M.R. Lewis, D. Radune, N.B. Fedorova, D. Scanlan, H. Khouri, S. Mulligan, H.A. Carty, R.T. Cline, S.E. Van Aken, J. Gill, M. Scarselli, M. Mora, E.T. Iacobini, C. Brettoni, G. Galli, M. Mariani, F. Vegni, D. Maione, D. Rinaudo, R. Rappuoli, J.L. Telford, D.L. Kasper, G. Grandi, and C.M. Fraser Complete genome sequence and comparative genomic analysis of an emerging human pathogen, serotype V Streptococcus agalactiae Proc. Natl. Acad. Sci. USA 99 2002 12391 12396
12. I.C. Sutcliffe, and D.H. Harrington Putative lipoproteins of Streptococcus agalactiae identified by bioinformatic genome analysis Antonie van Leeuwenhoek 85 2004 305 315
13. M.H. Lee, A. Nittayajarn, R.P. Ross, C.B. Rothschild, D. Parsonage, A. Claiborne, and C.E. Rubens Characterization of Enterococcus faecalis alkaline phosphatase and use in identifying Streptococcus agalactiae secreted proteins J. Bacteriol. 181 1999 5790 5799
14. C. Manoil, and J. Beckwith TnphoA: a transposon probe for protein export signals Proc. Natl. Acad. Sci. USA 82 1985 8129 8133
15. B. Pearce, Y. Yin, and H. Masure Genetic identification of exported proteins in Streptococcus pneumoniae Mol. Microbiol. 9 1993 1037 1050
16. P.E. Framson, A. Nittayajarn, J. Merry, P. Youngman, and C.E. Rubens New genetic techniques for group B streptococci: high-efficiency transformation, maintenance of temperature-sensitive pWV01 plasmids, and mutagenesis with Tn917 Appl. Environ. Microbiol. 63 1997 3539 3547
17. Z. Eichenbaum, and J.R. Scott Use of Tn917 to generate insertion mutations in the group A streptococcus Gene 186 1997 213 217
18. J.A. Gutierrez, P.J. Crowley, D.P. Brown, J.D. Hillman, P. Youngman, and A.S. Bleiweis Insertional mutagenesis and recovery of interrupted genes of Streptococcus mutans by using transposon Tn917: preliminary characterization of mutants displaying acid sensitivity and nutritional requirements J. Bacteriol. 178 1996 4166 4175
19. L. Grueter, O. Koenig, and R. Laufs Transposon mutagenesis in Staphylococcus epidermidis using the Enterococcus faecalis transposon Tn917 FEMS Microbiol. Lett. 66 1991 215 218
20. N.A. Bohall Jr., and P.S. Vary Transposition of Tn917 in Bacillus megaterium J. Bacteriol. 167 1986 716 718
21. J. Shaw, and D. Clewell Complete nucleotide sequence of macrolide-lincosamide-streptogramin B-resistance transposon Tn917 in Streptococcus faecalis J. Bacteriol. 164 1985 782 796
22. J. Perkins, and P. Youngman Construction and properties of Tn917 lac, a transposon derivative that mediates transcriptional gene fusions in Bacillus subtilis Proc. Natl. Acad. Sci. USA 83 1986 140 144
23. A.L. Jones, K.M. Knoll, and C.E. Rubens Identification of Streptococcus agalactiae virulence genes in the neonatal rat sepsis model using signature-tagged mutagenesis Mol. Microbiol. 37 2000 1444 1455
24. D.O. Chaffin, and C.E. Rubens Blue/white screening of recombinant plasmids in Gram-positive bacteria by interruption of alkaline phosphatase gene (phoZ) expression Gene 219 1998 91 99
25. F.M. Ausubel, R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith, and K. Struhl Current Protocols in Molecular Biology 1995 Wiley New York
26. C.M. Gibson, and M.G. Caparon Alkaline phosphatase reporter transposon for identification of genes encoding secreted proteins in gram-positive microorganisms Appl. Environ. Microbiol. 68 2002 928 932
27. B. Traxler, D. Boyd, and J. Beckwith The topological analysis of integral cytoplasmic membrane proteins J. Membr. Biol. 132 1993 1 11
28. L. Seligman, and C. Manoil An amphipathic sequence determinant of membrane protein topology J. Biol. Chem. 269 1994 19888 19896
29. A.I. Derman, and J. Beckwith Escherichia coli alkaline phosphatase localized to the cytoplasm slowly acquires enzymatic activity in cells whose growth has been suspended: a caution for gene fusion studies J. Bacteriol. 177 1995 3764 3770
30. M.J. Hughes, J.C. Moore, J.D. Lane, R. Wilson, P.K. Pribul, Z.N. Younes, R.J. Dobson, P. Everest, A.J. Reason, J.M. Redfern, F.M. Greer, T. Paxton, M. Panico, H.R. Morris, R.G. Feldman, and J.D. Santangelo Identification of major outer surface proteins of Streptococcus agalactiae Infect. Immun. 70 2002 1254 1259
31. N. Chanter, C.L. Ward, N.C. Talbot, J.A. Flanagan, M. Binns, S.B. Houghton, K.C. Smith, and J.A. Mumford Recombinant hyaluronate associated protein as a protective immunogen against Streptococcus equi and Streptococcus zooepidemicus challenge in mice Microb. Pathog. 27 1999 133 143
32. T. Tomoyasu, K. Yamanaka, K. Murata, T. Suzaki, P. Bouloc, A. Kato, H. Niki, S. Hiraga, and T. Ogura Topology and subcellular localization of FtsH protein in Escherichia coli J. Bacteriol. 175 1993 1352 1357
33. M. Chanyangam, A.L. Smith, S.L. Moseley, M. Kuehn, and P. Jenny Contribution of a 28-kilodalton membrane protein to the virulence of Haemophilus influenzae Infect. Immun. 59 1991 600 608
34. S. Chong, F.B. Mersha, D.G. Comb, M.E. Scott, D. Landry, L.M. Vence, F.B. Perler, J. Benner, R.B. Kucera, C.A. Hirvonen, J.J. Pelletier, H. Paulus, and M.Q. Xu Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element Gene 192 1997 277 281
35. K.F. Wertman, A.R. Wyman, and D. Botstein Host/vector interactions which affect the viability of recombinant phage lambda clones Gene 49 1986 253 262
36. L.C. Madoff, J.L. Michel, and D.L. Kasper A monoclonal antibody identifies a protective C-protein alpha-antigen epitope in group B streptococci Infect. Immun. 59 1991 204 210
37. T.R. Martin, C.E. Rubens, and C.B. Wilson Lung antibacterial defense mechanisms in infant and adult rats: implications for the pathogenesis of group B streptococcal infections in the neonatal lung J. Infect. Dis. 157 1988 91 100
38. S. Ballester, P. Lopez, J.C. Alonso, M. Espinosa, and S.A. Lacks Selective advantage of deletions enhancing chloramphenicol acetyltransferase gene expression in Streptococcus pneumoniae plasmids Gene 41 1986 153 163