Detection of Ralstonia solanacearum from potato tissue by post-enrichment TaqMan PCR

EPPO Bulletin

Volumn 30, Issue 3-4, 2000, Pages 381-383

  Weller, S A ^a   Elphinstone, J G ^a   Smith, N C ^a   Stead, D E ^a  

^a CENTRAL SCIENCE LABORATORY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 85024003554     PISSN: 02508052     EISSN: 13652338     Source Type: Journal    
DOI: 10.1111/j.1365-2338.2000.tb00915.x     Document Type: Article

References (7)

1. Elphinstone JG, Hennessy J, Wilson JK & Stead DE (1996) Sensitivity of different methods for the detection of Ralstonia solanacearum. Bulletin OEPP/EPPO Bulletin 26, 674–678.
2. EU (1998) Council Directive 98/57/EC. Official Journal of the European Communities L-235, 1–39.
3. Holland PM, Abramson RD, Watson R & Gelfand DH (1991) Detection of specific polymerase chain reaction product by utilizing the 5′ to 3′ exonuclease activity of Thermus aquaticus DNA polymerase. Proceedings of the National Academy of Science USA 88, 7276–7280.
4. Kelman A (1954) The relationship of pathogenicity in Pseudomonas solanacearum to colony appearance on the tetrazolium medium. Phytopathology 39, 936–946.
5. Lee LG, Cornell CR & Bloch W (1993) Allelic discrimination by nick-translation PCR with fluorogenic probes. Nucleic Acids Research 21, 3761–3766.
6. Schaad NW, Berthier-Schaad Y, Sechler A & Knorr D (1999) Detection of Clavibacter michiganensis subsp. sepedonicus in potato tubers by BIOPCR and an automated real time fluorescence detection system. Plant Diseased, 1095–1100.
7. Weller SA, Elphinstone JG, Smith N, Boonham N & Stead DE (2000) Detection of Ralstonia solanacearum strains using a quantitative, multiplex, real-time, fluorogenic PCR (TaqMan) assay. Applied and Environmental Microbiology 66, 2853–2858.